Monoclonal, hyperimmune rabbit and human serum anti-gliadin antibodies were analyzed by ELISA and immunoblotting techniques. In Western blotting the difference in reactivity between monoclonal and human antibodies was quantitative rather than qualitative. Rabbit antisera differed in reactivity according to the protein used for immunization. The rabbits immunized by the peptic-tryptic pancreatic digest of gliadin reacted similarly to the patients. In ELISA, significantly higher reactivity with crude, A-, glyc-gli, alpha-, beta- and omega-gliadins was found in the patients' sera than in controls.

• MeSH
• Immune Sera immunology   MeSH
• Enzyme-Linked Immunosorbent Assay MeSH
• Gliadin immunology   MeSH
• Immunization methods   MeSH
• Rabbits immunology   MeSH
• Humans MeSH
• Antibodies, Monoclonal immunology   isolation & purification   MeSH
• Mice immunology   MeSH
• Blotting, Western MeSH
• Animals MeSH
• Check Tag
• Rabbits immunology   MeSH
• Humans MeSH
• Mice immunology   MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Immune Sera MeSH
• Gliadin MeSH
• Antibodies, Monoclonal MeSH