Out of six samples of wastewater produced in the dairy industry, taken in 2017 at various places of dairy operations, 86 bacterial strains showing decarboxylase activity were isolated. From the wastewater samples, the species of genera Staphylococcus, Lactococcus, Enterococcus, Microbacterium, Kocuria, Acinetobacter, Pseudomonas, Aeromonas, Klebsiella and Enterobacter were identified by the MALDI-TOF MS and biochemical methods. The in vitro produced quantity of eight biogenic amines (BAs) was detected by the HPLC/UV-Vis method. All the isolated bacteria were able to produce four to eight BAs. Tyramine, putrescine and cadaverine belonged to the most frequently produced BAs. Of the isolated bacteria, 41% were able to produce BAs in amounts >100 mg L-1. Therefore, wastewater embodies a potential vector of transmission of decarboxylase positive microorganisms, which should be taken into consideration in hazard analyses within foodstuff safety control. The parameters of this wastewater (contents of nitrites, nitrates, phosphates, and proteins) were also monitored.

• Keywords
• bacteria, biogenic amines, decarboxylase activity, wastewater,
• MeSH
• Acinetobacter MeSH
• Aeromonas MeSH
• Biogenic Amines chemistry   MeSH
• Water Pollutants, Chemical chemistry   isolation & purification   MeSH
• Enterobacter MeSH
• Enterococcus MeSH
• Carboxy-Lyases chemistry   MeSH
• Klebsiella MeSH
• Lactobacillus MeSH
• Lactococcus MeSH
• Microbacterium MeSH
• Water Microbiology MeSH
• Dairying * MeSH
• Wastewater analysis   microbiology   MeSH
• Pediococcus MeSH
• Pseudomonas MeSH
• Spectrophotometry, Ultraviolet MeSH
• Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization MeSH
• Staphylococcus MeSH
• Streptococcus MeSH
• Chromatography, High Pressure Liquid MeSH
• Publication type
• Journal Article MeSH
• Geographicals
• Europe MeSH
• Names of Substances
• Biogenic Amines MeSH
• Water Pollutants, Chemical MeSH
• Carboxy-Lyases MeSH
• Waste Water MeSH

BACKGROUND: The objective of the study was to determine sensitivity of Enterococcus faecium strains from rabbit meat to enterocins. RESULTS: Twenty-five decarboxylase-positive strains (from rabbit meat) allotted to the species E. faecium by genotypization and by MALDI TOF MS spectrometry identification (evaluation score value range 2.104-2.359; in the range for highly probable species identification-score value 2.300-3.000 and secure probable species identification/probable species identification-2.000-2.299) were studied. Seventeen strains were gelatinase positive. Although they did not produce histamine (HIS), spermidine, and spermine, they produce at least one among seven tested biogenic amines (BAs) in small amounts (2-10 mg/L) or up to very high amounts (>1000 mg/L). Putrescine was produced by two strains. These decarboxylase-positive strains were sensitive to enterocins (Ents). All strains were sensitive to Ent 2019 and Ent 55 (inhibitory activity from 200 to 819 200 AU/mL). Twenty-two strains were inhibited by Ent A(P) and Ent 4231; 20 strains were sensitive to Ent M. CONCLUSION: Our results have spread the basic knowledge related to inhibitory spectrum of enterocins showing sensitivity of decarboxylase-positive strains to enterocins. Protective possibilities of enterocins in meat processing were also indicated.

• Keywords
• Decarboxylase, Enterocococci, enterocins, gelatinase, inhibition effect, rabbit meat,
• Publication type
• Journal Article MeSH