Out of six samples of wastewater produced in the dairy industry, taken in 2017 at various places of dairy operations, 86 bacterial strains showing decarboxylase activity were isolated. From the wastewater samples, the species of genera Staphylococcus, Lactococcus, Enterococcus, Microbacterium, Kocuria, Acinetobacter, Pseudomonas, Aeromonas, Klebsiella and Enterobacter were identified by the MALDI-TOF MS and biochemical methods. The in vitro produced quantity of eight biogenic amines (BAs) was detected by the HPLC/UV-Vis method. All the isolated bacteria were able to produce four to eight BAs. Tyramine, putrescine and cadaverine belonged to the most frequently produced BAs. Of the isolated bacteria, 41% were able to produce BAs in amounts >100 mg L-1. Therefore, wastewater embodies a potential vector of transmission of decarboxylase positive microorganisms, which should be taken into consideration in hazard analyses within foodstuff safety control. The parameters of this wastewater (contents of nitrites, nitrates, phosphates, and proteins) were also monitored.
- Klíčová slova
- bacteria, biogenic amines, decarboxylase activity, wastewater,
- MeSH
- Acinetobacter MeSH
- Aeromonas MeSH
- biogenní aminy chemie MeSH
- chemické látky znečišťující vodu chemie izolace a purifikace MeSH
- Enterobacter MeSH
- Enterococcus MeSH
- karboxylyasy chemie MeSH
- Klebsiella MeSH
- Lactobacillus MeSH
- Lactococcus MeSH
- Microbacterium MeSH
- mikrobiologie vody MeSH
- mlékárenství * MeSH
- odpadní voda analýza mikrobiologie MeSH
- Pediococcus MeSH
- Pseudomonas MeSH
- spektrofotometrie ultrafialová MeSH
- spektrometrie hmotnostní - ionizace laserem za účasti matrice MeSH
- Staphylococcus MeSH
- Streptococcus MeSH
- vysokoúčinná kapalinová chromatografie MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Evropa MeSH
- Názvy látek
- biogenní aminy MeSH
- chemické látky znečišťující vodu MeSH
- karboxylyasy MeSH
- odpadní voda MeSH
Pediococcus pentosaceus GS4 (MTCC 12683), a probiotic lactic acid bacterium (LAB), was found to produce bacteriocin in spent culture. Antibacterial and antagonistic potential of this bacteriocin against reference strains of Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 25619), and Listeria monocytogenes (ATCC 15313) was proven by double-layer and well diffusion methods wherein nisin and ampicillin were used as positive controls. Bacteriocin in supernatant was purified and analyzed by SDS-PAGE, RP-HPLC, and circular dichroism (CD). The physico-chemical properties of purified bacteriocin were characterized being treated at different temperatures (30 to 110 °C), pH (3.0 to 12.0), with different enzymes (α-amylase, pepsin, and lysozyme), and organic solvents (hexane, ethanol, methanol, and acetone) respectively. The molar mass of bacteriocin (named pediocin GS4) was determined as 9.57 kDa. The single peak appears at the retention time of 2.403 with area amounting to 25.02% with nisin as positive control in RP-HPLC. CD analysis reveals that the compound appears to have the helix ratio of 40.2% with no beta sheet. The antibacterial activity of pediocin GS4 was optimum at 50 °C and at pH 5.0 and 7.0. The pediocin GS4 was not denatured by the treatment of amylase and lysozyme but was not active in the presence of organic solvents. This novel bacteriocin thus m ay be useful in food and health care industry.
- MeSH
- antibakteriální látky chemie izolace a purifikace farmakologie MeSH
- Bacteria účinky léků MeSH
- hydrolasy metabolismus MeSH
- koncentrace vodíkových iontů MeSH
- molekulová hmotnost MeSH
- nisin chemie MeSH
- pediociny chemie izolace a purifikace farmakologie MeSH
- Pediococcus pentosaceus chemie metabolismus MeSH
- probiotika * MeSH
- rozpouštědla MeSH
- sekundární struktura proteinů MeSH
- stabilita proteinů MeSH
- teplota MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antibakteriální látky MeSH
- hydrolasy MeSH
- nisin MeSH
- pediociny MeSH
- rozpouštědla MeSH
Fungi are commonly involved in dairy product spoilage and the use of bioprotective cultures can be a complementary approach to reduce food waste and economic losses. In this study, the antifungal activity of 89 Lactobacillus and 23 Pediococcus spp. isolates against three spoilage species, e.g., Yarrowia lipolytica, Rhodotorula mucilaginosa and Penicillium brevicompactum, was first evaluated in milk agar. None of the tested pediococci showed antifungal activity while 3, 23 and 43 lactobacilli isolates showed strong antifungal activity or total inhibition against Y. lipolytica, R. mucilaginosa and P. brevicompactum, respectively. Then, the three most promising strains, Lactobacillus paracasei SYR90, Lactobacillus plantarum OVI9 and Lactobacillus rhamnosus BIOIII28 at initial concentrations of 105 and 107 CFU/ml were tested as bioprotective cultures against the same fungal targets in a yogurt model during a 5-week storage period at 10 °C. While limited effects were observed at 105 CFU/ml inoculum level, L. paracasei SYR90 and L. rhamnosus BIOIII28 at 107 CFU/ml respectively retarded the growth of R. mucilaginosa and P. brevicompactum as compared to a control without selected cultures. In contrast, growth of Y. lipolytica was only slightly affected. In conclusion, these selected strains may be good candidates for bioprotection of fermented dairy products.
- Klíčová slova
- Antifungal, Bioprotection, Dairy product, Fungi, Lactobacilli, Pediococci,
- MeSH
- antifungální látky farmakologie MeSH
- Lactobacillus fyziologie MeSH
- mléčné výrobky mikrobiologie MeSH
- Pediococcus fyziologie MeSH
- Penicillium růst a vývoj MeSH
- potravinářská mikrobiologie MeSH
- probiotika MeSH
- Rhodotorula růst a vývoj MeSH
- Yarrowia růst a vývoj MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antifungální látky MeSH
Biosensors based on lactate oxidase, sarcosine oxidase and mixture of fumarase and sarcosine oxidase were used for monitoring of organic acids in wine samples. Additionally, tartaric acid was determined by modified colorimetric method based on formation of the vanadate-tartrate complex. The above mentioned methods were used for the analysis of 31 wine samples and obtained data were compared with the results from capillary electrophoresis as a basic standard method. This comparison showed a certain degree of correlation between biosensors and capillary electrophoresis. The provided information pointed to the potential uses of biosensors in the field of winemaking.
- Klíčová slova
- Biosensor, Capillary electrophoresis, Carboxylic acids, Wine,
- MeSH
- analýza potravin přístrojové vybavení metody MeSH
- Bacillus enzymologie MeSH
- biosenzitivní techniky metody MeSH
- elektrody MeSH
- elektroforéza kapilární metody MeSH
- fermentace MeSH
- kalibrace MeSH
- kolorimetrie metody MeSH
- kyselina mléčná chemie MeSH
- kyseliny analýza MeSH
- maláty chemie MeSH
- myokard enzymologie MeSH
- organické látky analýza MeSH
- oxygenasy se smíšenou funkcí chemie MeSH
- Pediococcus enzymologie MeSH
- polymery chemie MeSH
- prasata MeSH
- sarkosinoxidasa chemie MeSH
- tartaráty chemie MeSH
- víno analýza MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- kyselina mléčná MeSH
- kyseliny MeSH
- lactate 2-monooxygenase MeSH Prohlížeč
- maláty MeSH
- malic acid MeSH Prohlížeč
- organické látky MeSH
- oxygenasy se smíšenou funkcí MeSH
- polymery MeSH
- sarkosinoxidasa MeSH
- tartaráty MeSH
- tartaric acid MeSH Prohlížeč
A large-scale screen of some 7,000 presumptive lactic acid bacteria (LAB), isolated from animal, human, or plant origin, identified 1,149 isolates with inhibitory activity against the food-spoilage mould Penicillium expansum. In excess of 500 LAB isolates were subsequently identified to produce a broad spectrum of activity against P. expansum, Penicillium digitatum, Penicillium notatum, Penicillium roqueforti, Rhizopus stolonifer, Fusarium culmorum, Aspergillus fumigatus and Rhodotorula mucilaginosa. Partial 16S rRNA sequencing of 94 broad spectrum isolates revealed that the majority of antifungal producers were strains of Lactobacillus plantarum. The remaining population was composed of Weissella confusa and Pediococcus pentosaceous isolates. Characterization of six selected broad-spectrum antifungal LAB isolates revealed that antifungal activity is maximal at a temperature of 30 °C, a pH of 4.0 and is stable across a variety of salt concentrations. The antifungal compound(s) was shown to be neither proteinaceous nor volatile in nature. P. pentosaceous 54 was shown to have protective properties against P. expansum spoilage when applied in pear, plum and grape models, therefore representing an excellent candidate for food-related applications.
- MeSH
- antifungální látky metabolismus MeSH
- DNA bakterií chemie genetika MeSH
- houby účinky léků růst a vývoj MeSH
- koncentrace vodíkových iontů MeSH
- konzervace potravin metody MeSH
- Lactobacillus plantarum klasifikace genetika izolace a purifikace metabolismus MeSH
- mikrobiální interakce MeSH
- ovoce mikrobiologie MeSH
- Pediococcus klasifikace genetika izolace a purifikace metabolismus MeSH
- potravinářská mikrobiologie MeSH
- Pyrus mikrobiologie MeSH
- ribozomální DNA chemie genetika MeSH
- RNA ribozomální 16S genetika MeSH
- sekvenční analýza DNA MeSH
- slivoň mikrobiologie MeSH
- teplota MeSH
- Vitis mikrobiologie MeSH
- Weissella klasifikace genetika izolace a purifikace metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antifungální látky MeSH
- DNA bakterií MeSH
- ribozomální DNA MeSH
- RNA ribozomální 16S MeSH
The effect of immersion time and cell concentration in the attachment of several lactic acid bacteria with antibacterial activity to beef-muscle surface was studied. The number of firmly attached bacteria increased with immersion time in the case of Pediococcus acidilacti, Lactobacillus sake, Lactococcus cremoris (two strains) and Pediococcus acidilacti. Pediococcus pentosaceus, Lactococcus lactis and Lactobacillus curvatus reached maximum adhesion after 15-30 min. The highest strength of attachment (Sr values) were observed after 15-30 min of contact time except for P. pentosaceus. For all strains, the number of bacteria adhering to meat increased with increasing cell concentration in the adhesion medium. The highest strength of attachment was observed at a cell concentration of 10(5)/mL mainly for L. sake, L. lactis and L. cremoris. Due to their attachment characteristics, L. sake, L. lactis and L. cremoris are proposed as potential biocontrol agents because they could grow on meat surface and limit the potential attachment of pathogenic microorganisms.
- MeSH
- bakteriální adheze * MeSH
- kyselina mléčná metabolismus MeSH
- Lactobacillus metabolismus MeSH
- Lactococcus metabolismus MeSH
- Leuconostoc metabolismus MeSH
- maso mikrobiologie MeSH
- Pediococcus metabolismus MeSH
- skot MeSH
- techniky in vitro MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- kyselina mléčná MeSH
A kinetic approach is proposed to shorten the microbiological assay time for the determination of unbound L-lysine. The present lysine bacterial assay takes from 16 to 24 h using Pediococcus cerevisiae P-60 ATCC 8042 (formerly Leuconostoc mesenteroides P-60 ATCC 8042) and uses a medium in which lysine is the limiting substance. Measurements of the final cell concentration are linearly correlated with the initial concentration of lysine, S, to provide an indirect estimate of S. We propose to understand the limitations inherent to the reduction of the assay time to 4 h by focusing in our analysis on the bacterial late lag or early growth transient phases, rather than the stationary phase of growth. Generally, the Monod equation is expected to describe a hyperbolically increasing correlation between the bacterial specific growth rate at about 2-4 h and the initial lysine concentration. A hyperbolic correlation is obtained by 3 h, but the lysine region of interest falls in the saturated portion of the curve. Discriminations between different initial lysine levels are therefore difficult with this nearly flat curve. On the other hand, when the initial inoculum level is lowered, so that substrate inhibition becomes effective, a correlation with a large negative slope is obtained by 4 h. Limitations to using absorbance measurements for the rapid assay turn up in a lack of reproducibility and, hence, a large variance associated with the measurements. Alternative microbial measuring techniques, such as impedance methods, need to be examined in order to reduce that large variance.
- MeSH
- biologické modely MeSH
- biotest metody MeSH
- lysin analýza metabolismus MeSH
- Pediococcus růst a vývoj metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- lysin MeSH
- MeSH
- bakteriologické techniky MeSH
- chromatografie MeSH
- fermentace MeSH
- glukosa metabolismus MeSH
- jedlé rostliny MeSH
- koncentrace vodíkových iontů MeSH
- krmivo pro zvířata * MeSH
- Lactobacillaceae izolace a purifikace metabolismus MeSH
- Lactobacillus izolace a purifikace MeSH
- Lactococcus lactis izolace a purifikace MeSH
- Leuconostoc izolace a purifikace MeSH
- mikrobiální testy citlivosti MeSH
- Pediococcus izolace a purifikace MeSH
- potravinářská mikrobiologie * MeSH
- siláž * MeSH
- uhličitan vápenatý metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- glukosa MeSH
- uhličitan vápenatý MeSH
