Degradation of undesirable biogenic amines (BAs) in foodstuffs by microorganisms is considered one of the most effective ways of eliminating their toxicity. In this study, we design two sets of primers for the detection and quantification of the amine oxidase gene (yobN) and endogenous (housekeeping) gene (gyrB) in Bacillus subtilis. Moreover, these sets can be used for relative quantification of yobN by real-time PCR (qPCR). We also tested the degradation of BAs by three bacterial strains (B. subtilis strains: IB1a, CCM 2216, CCM 2267) in a mineral medium over a two-day period. Their degradation abilities were verified by high performance liquid chromatography with UV detection (HPLC/UV). According to the results, two strains significantly (P < 0.05) reduced histamine, tyramine, putrescine, and cadaverine. Moreover, our results indicate that the degradation ability of B. subtilis strains could be limited by sporulation because the gene encoding amine oxidase (yobN) is no longer expressed in the spores.

• Klíčová slova
• Biogenic amines degradation, Histamine, Primers, Real-time PCR,
• Publikační typ
• časopisecké články MeSH

Out of six samples of wastewater produced in the dairy industry, taken in 2017 at various places of dairy operations, 86 bacterial strains showing decarboxylase activity were isolated. From the wastewater samples, the species of genera Staphylococcus, Lactococcus, Enterococcus, Microbacterium, Kocuria, Acinetobacter, Pseudomonas, Aeromonas, Klebsiella and Enterobacter were identified by the MALDI-TOF MS and biochemical methods. The in vitro produced quantity of eight biogenic amines (BAs) was detected by the HPLC/UV-Vis method. All the isolated bacteria were able to produce four to eight BAs. Tyramine, putrescine and cadaverine belonged to the most frequently produced BAs. Of the isolated bacteria, 41% were able to produce BAs in amounts >100 mg L-1. Therefore, wastewater embodies a potential vector of transmission of decarboxylase positive microorganisms, which should be taken into consideration in hazard analyses within foodstuff safety control. The parameters of this wastewater (contents of nitrites, nitrates, phosphates, and proteins) were also monitored.

• Klíčová slova
• bacteria, biogenic amines, decarboxylase activity, wastewater,
• MeSH
• Acinetobacter MeSH
• Aeromonas MeSH
• biogenní aminy chemie   MeSH
• chemické látky znečišťující vodu chemie   izolace a purifikace   MeSH
• Enterobacter MeSH
• Enterococcus MeSH
• karboxylyasy chemie   MeSH
• Klebsiella MeSH
• Lactobacillus MeSH
• Lactococcus MeSH
• Microbacterium MeSH
• mikrobiologie vody MeSH
• mlékárenství * MeSH
• odpadní voda analýza   mikrobiologie   MeSH
• Pediococcus MeSH
• Pseudomonas MeSH
• spektrofotometrie ultrafialová MeSH
• spektrometrie hmotnostní - ionizace laserem za účasti matrice MeSH
• Staphylococcus MeSH
• Streptococcus MeSH
• vysokoúčinná kapalinová chromatografie MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Evropa MeSH
• Názvy látek
• biogenní aminy MeSH
• chemické látky znečišťující vodu MeSH
• karboxylyasy MeSH
• odpadní voda MeSH