Liposome-based drug delivery systems hold great potential for cancer therapy. The aim of this study was to design a nanodevice for targeted anchoring of liposomes (with and without cholesterol) with encapsulated anticancer drugs and antisense N-myc gene oligonucleotide attached to its surface. To meet this main aim, liposomes with encapsulated doxorubicin, ellipticine and etoposide were prepared. They were further characterized by measuring their fluorescence intensity, whereas the encapsulation efficiency was estimated to be 16%. The hybridization process of individual oligonucleotides forming the nanoconstruct was investigated spectrophotometrically and electrochemically. The concentrations of ellipticine, doxorubicin and etoposide attached to the nanoconstruct in gold nanoparticle-modified liposomes were found to be 14, 5 and 2 µg·mL(-1), respectively. The study succeeded in demonstrating that liposomes are suitable for the transport of anticancer drugs and the antisense oligonucleotide, which can block the expression of the N-myc gene.

• Klíčová slova
• N-myc, doxorubicin, ellipticine, etoposide, gold nanoparticles, liposome,
• MeSH
• antisense DNA chemie   terapeutické užití   MeSH
• doxorubicin chemie   terapeutické užití   MeSH
• elipticiny chemie   terapeutické užití   MeSH
• etoposid chemie   terapeutické užití   MeSH
• fluorescence MeSH
• lidé MeSH
• liposomy chemie   terapeutické užití   MeSH
• magnetické nanočástice chemie   terapeutické užití   MeSH
• nádory farmakoterapie   MeSH
• protoonkogen n-myc antagonisté a inhibitory   genetika   MeSH
• systémy cílené aplikace léků * MeSH
• zlato chemie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antisense DNA MeSH
• doxorubicin MeSH
• elipticiny MeSH
• etoposid MeSH
• liposomy MeSH
• magnetické nanočástice MeSH
• protoonkogen n-myc MeSH
• zlato MeSH

Increasing urbanization and industrialization lead to the release of metals into the biosphere, which has become a serious issue for public health. In this paper, the direct electrochemical reduction of zinc ions is studied using electrochemically reduced graphene oxide (ERGO) modified glassy carbon electrode (GCE). The graphene oxide (GO) was fabricated using modified Hummers method and was electrochemically reduced on the surface of GCE by performing cyclic voltammograms from 0 to -1.5 V. The modification was optimized and properties of electrodes were determined using electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The determination of Zn(II) was performed using differential pulse voltammetry technique, platinum wire as a counter electrode, and Ag/AgCl/3 M KCl reference electrode. Compared to the bare GCE the modified GCE/ERGO shows three times better electrocatalytic activity towards zinc ions, with an increase of reduction current along with a negative shift of reduction potential. Using GCE/ERGO detection limit 5 ng·mL-1 was obtained.

• Klíčová slova
• carbon, cyclic voltammetry, electrochemical impedance spectroscopy, electrochemistry, graphene oxide, heavy metal detection, reduced graphene oxide,
• Publikační typ
• časopisecké články MeSH

Cisplatin belongs to the most widely used cytostatic drugs. The determination of the presence of the DNA-cisplatin adducts may not only signal the guanine-rich regions but also monitor the interaction reaction between DNA and the drug in terms of speed of interaction. In this work, the combined advantages of magnetic particles-based isolation/purification with fluorescent properties of quantum dots (QDs) and antibodies targeted on specific recognition of DNA-cisplatin adducts are demonstrated. The formation of a complex between magnetic particles with surface modified by anti-dsDNA antibody, cisplatin-modified DNA and QDs labelled anti-cisplatin-modified DNA antibody was suggested and optimized.

• Klíčová slova
• Anti-DNA Antibodies, Cisplatin, Magnetic Separation, Sandwich Analysis,
• Publikační typ
• časopisecké články MeSH