Most cited article - PubMed ID 23984
Transport kinetics of 6-deoxy-D-glucose in Candida parapsilosis
A plant-sap-derived preparation containing bi- and tervalent ferrate anions was tested on growth, respiration on glucose, and membrane transport of 6-deoxy-D-glucose (6-dGlc) and 2-aminoisobutyric acid (Aib) in several yeast species, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Lodderomyces elongisporus, Rhodotorula gracilis, and Dipodascus magnusii. Growth was enhanced by as much as 65%, respiration was not affected significantly except for a decrease in R. gracilis, transport of 6-dGlc was not affected while that of Aib was increased by up to 45% in R. gracilis and up to 27% in L. elongisporus.
- MeSH
- Biological Transport, Active drug effects MeSH
- Cell Membrane drug effects metabolism MeSH
- Deoxyglucose analogs & derivatives metabolism MeSH
- Kinetics MeSH
- Yeasts drug effects growth & development metabolism MeSH
- Aminoisobutyric Acids metabolism MeSH
- Plant Extracts chemistry pharmacology MeSH
- Oxygen Consumption drug effects MeSH
- Iron pharmacology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 2-aminoisobutyric acid MeSH Browser
- 6-deoxyglucose MeSH Browser
- Deoxyglucose MeSH
- ferrate ion MeSH Browser
- Aminoisobutyric Acids MeSH
- Plant Extracts MeSH
- Iron MeSH
Most nutrients and ions in bacteria, yeasts, algae, and plants are transported uphill at the expense of a gradient of the electrochemical potential of protons deltamu-H+ (a type of secondary active transport). Diagnosis of such transports rests on the determination of the transmembrane electrical potential difference deltapsi and the difference of pH at the two membrane sides. The behavior of kinetic parameters K(T) (the half-saturation constant) and J(max), (the maximum rate of transport) upon changing driving ion concentrations and electrical potentials may be used to determine the molecular details of the transport reaction. Equilibrium accumulation ratios of driven solutes are expected to be in agreement with the deltapsi and deltapH measured independently, as well as with the Haldane-type expression involving K(T) and J(max). Different stoichiometries of H+/solute, as well as intramembrane effects of pH and deltapsi, may account for some of the observed inconsistencies.
