Monoclonal antibodies (mAb) to the transmissible gastroenteritis virus (TGEV) nucleoprotein (N) and membrane protein (M) were prepared and used for the comparative assessment of three blocking ELISA variants to detect TGEV. The competitive blocking ELISA format showed the highest sensitivity, allowing detection of 10(3) TCID50 TGEV/ml in culture medium. Ninety-nine porcine field faecal samples obtained from 37 herds affected with diarrhoea were examined, and various TGEV levels were found in nine samples from six herds. However, only in three samples were significant TGEV concentrations demonstrated. The relationship between incidence of TGEV gastroenteritis and the spread of porcine respiratory coronavirus infection in pig farms is discussed.

• MeSH
• antigeny virové analýza   MeSH
• ELISA metody   veterinární   MeSH
• feces virologie   MeSH
• monoklonální protilátky * imunologie   MeSH
• myši MeSH
• náhodné rozdělení MeSH
• novorozená zvířata MeSH
• nukleokapsida - proteiny imunologie   MeSH
• prasata MeSH
• přenosná gastroenteritida prasat diagnóza   MeSH
• proteiny virové matrix imunologie   MeSH
• protilátky virové imunologie   MeSH
• průjem diagnóza   veterinární   virologie   MeSH
• senzitivita a specificita MeSH
• virus přenosné gastroenteritidy prasat imunologie   izolace a purifikace   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antigeny virové MeSH
• monoklonální protilátky * MeSH
• nukleokapsida - proteiny MeSH
• proteiny virové matrix MeSH
• protilátky virové MeSH

Monoclonal antibodies to porcine epidemic diarrhoea virus (PEDV) membrane protein M were prepared and used for the comparative assessment of three blocking ELISA variants to detect PEDV. The competitive blocking ELISA (CB-ELISA) format showed the highest sensitivity, allowing detection of 10(2.5) plaque-forming units of PEDV/ml in culture medium. Its specificity was verified by inclusion of control samples containing transmissible gastroenteritis virus (TGEV) and rotavirus A in each analysis. Eighty porcine field samples of faeces obtained from 38 herds affected with diarrhoea were examined, and PEDV was found in 15 (19%) samples from 6 (16%) herds. The suitability of the CB-ELISA for the screening herds in epizootiologic situations is discussed.

• MeSH
• Coronavirus izolace a purifikace   MeSH
• elektroforéza v polyakrylamidovém gelu veterinární   MeSH
• elektronová mikroskopie veterinární   MeSH
• ELISA metody   veterinární   MeSH
• feces virologie   MeSH
• imunoenzymatické techniky veterinární   MeSH
• koronavirové infekce diagnóza   veterinární   virologie   MeSH
• monoklonální protilátky imunologie   MeSH
• nemoci prasat diagnóza   virologie   MeSH
• prasata MeSH
• proteiny virové matrix imunologie   MeSH
• protilátky blokující imunologie   MeSH
• průjem diagnóza   veterinární   virologie   MeSH
• senzitivita a specificita MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• Názvy látek
• monoklonální protilátky MeSH
• proteiny virové matrix MeSH
• protilátky blokující MeSH

Individual beta-tubulin isoforms in developing mouse brain were characterized using immunoblotting, after preceding high-resolution isoelectric focusing, with monoclonal antibodies against different structural regions of beta-tubulin. Some of the antibodies reacted with a limited number of tubulin isoforms in all stages of brain development and in HeLa cells. The epitope for the TU-14 antibody was located in the isotype-defining domain and was present on the beta-tubulin isotypes of classes I, II and IV, but absent on the neuron-specific class-III isotype. The data suggest that non-class-III beta-tubulins in mouse brain are substrates for developmentally regulated post-translational modifications and that beta-tubulins of non-neuronal cells are also post-translationally modified.

• MeSH
• epitopy analýza   MeSH
• HeLa buňky MeSH
• imunoblotting MeSH
• isoelektrická fokusace MeSH
• isomerie MeSH
• lidé MeSH
• molekulární sekvence - údaje MeSH
• monoklonální protilátky MeSH
• mozek růst a vývoj   imunologie   metabolismus   MeSH
• myši inbrední BALB C MeSH
• myši MeSH
• posttranslační úpravy proteinů fyziologie   MeSH
• sekvence aminokyselin MeSH
• tubulin imunologie   metabolismus   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• epitopy MeSH
• monoklonální protilátky MeSH
• tubulin MeSH

Expression of intermediate filament proteins was studied in human developing spinal cord using immunoperoxidase and double-label immunofluorescence methods with monoclonal antibodies to vimentin and glial fibrillary acidic protein (GFAP). Vimentin was found in the processes of radial glial cells in 6-week embryos, while GFAP appeared in vimentin-positive astroglial cells at 8-10 weeks. GFAP and vimentin were present in approximately equal amounts in differentiating astrocytes in 23-week spinal cord. In 30-week fetuses, astrocytes reacted strongly for GFAP, while both the reaction intensity and the number of vimentin-positive cells fluctuated predominantly in the grey matter. No clear-cut transition from vimentin to GFAP was noticed during the development of astrocytes. The majority of ependymal cells in 23-week fetuses contained vimentin but only a few of them reacted for GFAP. The expression of vimentin continued during the whole development of the ependymal layer, in contrast to the reactivity for GFAP which disappeared between the 30th week and term.

• MeSH
• barvení a značení MeSH
• elektroforéza v polyakrylamidovém gelu MeSH
• exprese genu MeSH
• fluorescenční mikroskopie MeSH
• fluorescenční protilátková technika MeSH
• gestační stáří MeSH
• gliový fibrilární kyselý protein genetika   imunologie   MeSH
• lidé MeSH
• mícha embryologie   metabolismus   MeSH
• monoklonální protilátky MeSH
• těhotenství MeSH
• vimentin genetika   imunologie   MeSH
• Check Tag
• lidé MeSH
• těhotenství MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• gliový fibrilární kyselý protein MeSH
• monoklonální protilátky MeSH
• vimentin MeSH