Monoclonal antibodies (mAb) to the transmissible gastroenteritis virus (TGEV) nucleoprotein (N) and membrane protein (M) were prepared and used for the comparative assessment of three blocking ELISA variants to detect TGEV. The competitive blocking ELISA format showed the highest sensitivity, allowing detection of 10(3) TCID50 TGEV/ml in culture medium. Ninety-nine porcine field faecal samples obtained from 37 herds affected with diarrhoea were examined, and various TGEV levels were found in nine samples from six herds. However, only in three samples were significant TGEV concentrations demonstrated. The relationship between incidence of TGEV gastroenteritis and the spread of porcine respiratory coronavirus infection in pig farms is discussed.

• MeSH
• antigeny virové analýza   MeSH
• ELISA metody   veterinární   MeSH
• feces virologie   MeSH
• monoklonální protilátky * imunologie   MeSH
• myši MeSH
• náhodné rozdělení MeSH
• novorozená zvířata MeSH
• nukleokapsida - proteiny imunologie   MeSH
• prasata MeSH
• přenosná gastroenteritida prasat diagnóza   MeSH
• proteiny virové matrix imunologie   MeSH
• protilátky virové imunologie   MeSH
• průjem diagnóza   veterinární   virologie   MeSH
• senzitivita a specificita MeSH
• virus přenosné gastroenteritidy prasat imunologie   izolace a purifikace   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antigeny virové MeSH
• monoklonální protilátky * MeSH
• nukleokapsida - proteiny MeSH
• proteiny virové matrix MeSH
• protilátky virové MeSH

Monoclonal antibodies to porcine epidemic diarrhoea virus (PEDV) membrane protein M were prepared and used for the comparative assessment of three blocking ELISA variants to detect PEDV. The competitive blocking ELISA (CB-ELISA) format showed the highest sensitivity, allowing detection of 10(2.5) plaque-forming units of PEDV/ml in culture medium. Its specificity was verified by inclusion of control samples containing transmissible gastroenteritis virus (TGEV) and rotavirus A in each analysis. Eighty porcine field samples of faeces obtained from 38 herds affected with diarrhoea were examined, and PEDV was found in 15 (19%) samples from 6 (16%) herds. The suitability of the CB-ELISA for the screening herds in epizootiologic situations is discussed.

• MeSH
• Coronavirus izolace a purifikace   MeSH
• elektroforéza v polyakrylamidovém gelu veterinární   MeSH
• elektronová mikroskopie veterinární   MeSH
• ELISA metody   veterinární   MeSH
• feces virologie   MeSH
• imunoenzymatické techniky veterinární   MeSH
• koronavirové infekce diagnóza   veterinární   virologie   MeSH
• monoklonální protilátky imunologie   MeSH
• nemoci prasat diagnóza   virologie   MeSH
• prasata MeSH
• proteiny virové matrix imunologie   MeSH
• protilátky blokující imunologie   MeSH
• průjem diagnóza   veterinární   virologie   MeSH
• senzitivita a specificita MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• Názvy látek
• monoklonální protilátky MeSH
• proteiny virové matrix MeSH
• protilátky blokující MeSH

Concanavalin A (ConA) binding to lipopigments (LPs) of the lipofuscin type was proved to be due to the high content of mannose. The nature of the mannose bearing compound was twofold. One part was soluble in modified chloroform-methanol-water mixture (10:10:3) corresponding possibly to the oligosaccharyl diphosphodolichol (oligo-PP-Dol) described to be increased in LPs especially of inherited types. The second part, most probably a glycoprotein (GP), was entirely resistant to various extraction procedures. The ratio of the two components varied. The deposition of the typical lipofuscin (age pigment) was dominated by the GP component. Its amount was greatest in neurolipofuscin (especially in the olivary nucleus) and in the myocardium but very little in hepatocytic lipofuscin. In human neuronal ceroid lipofuscinoses (of early juvenile, and juvenile types) both components were found in large quantities in the storage granules of the affected neurons. The "protein type variant" of the storage material (Elleder 1978) displayed the highest degree of lipid-bound mannose accumulation, the GP component being extremely low or entirely absent. In the late infantile, infantile and Kufs variants studied in paraffin sections only, the GP component was detectable, too as in the case of the secondary neuronal LP in mucopolysaccharidoses and gangliosidoses. In the dog model of NCL lipid bound mannose clearly predominated, the GP component being concentrated in the cytoplasm and on the periphery od some storage granules. The nature of the GP component, a new finding of LP analysis, is discussed. The metabolic relationship between the two components is uncertain. Neither could be identified as the component resposible for autofluorescence.(ABSTRACT TRUNCATED AT 250 WORDS)

• MeSH
• barvení a značení * MeSH
• biologické pigmenty analýza   MeSH
• ceroid analýza   metabolismus   MeSH
• dítě MeSH
• dospělí MeSH
• gangliosidóza patologie   MeSH
• konkanavalin A * metabolismus   MeSH
• lidé MeSH
• lipofuscin analýza   metabolismus   MeSH
• mannosa metabolismus   MeSH
• mladiství MeSH
• mukopolysacharidózy patologie   MeSH
• neuronální ceroidlipofuscinózy metabolismus   patologie   MeSH
• neurony analýza   MeSH
• nucleus olivaris caudalis analýza   MeSH
• předškolní dítě MeSH
• zvířata MeSH
• Check Tag
• dítě MeSH
• dospělí MeSH
• lidé MeSH
• mladiství MeSH
• předškolní dítě MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• biologické pigmenty MeSH
• ceroid MeSH
• konkanavalin A * MeSH
• lipofuscin MeSH
• mannosa MeSH