The T-lymphocyte co-receptors of MHC glycoproteins CD4 and CD8 are known to be associated with the protein tyrosine kinase Lck via cysteine-containing sequences in the cytoplasmic domains of CD4 and CD8 and in the N-terminal domain of Lck. Here we demonstrate that a fraction of CD4 and CD8 molecules are associated with very large, detergent-resistant complexes containing several glycosylphosphatidylinositol-anchored proteins, (glyco)lipids, and protein tyrosine kinases Lck and Fyn but apparently no other major transmembrane proteins. Association of Lck and Fyn with these large complexes is, in contrast to simple CD4/CD8-Lck complexes, not sensitive to alkylation with iodoacetamide. These large complexes therefore represent an alternative way of association of CD4 and CD8 with the protein tyrosine kinases, which may play a role in signaling through these receptors.

• MeSH
• antigeny CD4 analýza   MeSH
• antigeny CD8 analýza   MeSH
• buněčná membrána chemie   MeSH
• glykosylfosfatidylinositoly MeSH
• lidé MeSH
• lymfocyty chemie   MeSH
• nádorové proteiny * MeSH
• tyrosinkinasa p56(lck), specifická pro lymfocyty MeSH
• tyrosinkinasy analýza   klasifikace   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antigeny CD4 MeSH
• antigeny CD8 MeSH
• FRK protein, human MeSH Prohlížeč
• glykosylfosfatidylinositoly MeSH
• nádorové proteiny * MeSH
• tyrosinkinasa p56(lck), specifická pro lymfocyty MeSH
• tyrosinkinasy MeSH

Melanoma cells freshly isolated from regional lymph node metastases of 59 stage II malignant melanoma patients were analyzed by indirect immunofluorescence staining with monoclonal antibody TAL 1B5, detecting the HLA-DR alpha chain. The expression of HLA-A,B,C antigens, using antibody W6/32, was also investigated in 45 of these cases. There were no substantial differences in the course of the disease with respect to the percentage of positive cells. In 13 malignant melanoma patients two to four simultaneous and/or successive metastases (both locoregional and distant-subcutaneous) were analysed for HLA-DR. With simultaneous metastases (7 cases) the percentage of HLA-DR-positive cells was mostly very similar, and in no case was there more than 25% variation. As to successive metastases (9 cases) the percentage of HLA-DR-positive cells remained practically unchanged or decreased during the course of the disease.

• MeSH
• dospělí MeSH
• HLA-DR antigeny analýza   MeSH
• lidé středního věku MeSH
• lidé MeSH
• makrofágy imunologie   MeSH
• melanom imunologie   MeSH
• metastázy nádorů MeSH
• prognóza MeSH
• senioři MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• HLA-DR antigeny MeSH