In the food industry, the increasing antimicrobial resistance of food-borne pathogens to conventional sanitizers poses the risk of food contamination and a decrease in product quality and safety. Therefore, we explored alternative antimicrobials N-Acetyl-l-cysteine (NAC), rhamnolipids (RLs), and usnic acid (UA) as a novel approach to prevent biofilm formation and reduce existing biofilms formed by important food-borne pathogens (three strains of Salmonella enterica and two strains of Escherichia coli, Listeria monocytogenes, Staphylococcus aureus). Their effectiveness was evaluated by determining minimum inhibitory concentrations needed for inhibition of bacterial growth, biofilm formation, metabolic activity, and biofilm reduction. Transmission electron microscopy and confocal scanning laser microscopy followed by image analysis were used to visualize and quantify the impact of tested substances on both planktonic and biofilm-associated cells. The in vitro cytotoxicity of the substances was determined as a half-maximal inhibitory concentration in five different cell lines. The results indicate relatively low cytotoxic effects of NAC in comparison to RLs and UA. In addition, NAC inhibited bacterial growth for all strains, while RLs showed overall lower inhibition and UA inhibited only the growth of Gram-positive bacteria. Even though tested substances did not remove the biofilms, NAC represents a promising tool in biofilm prevention.

• Klíčová slova
• N-Acetyl-l-cysteine, acute cytotoxicity, antimicrobial efficacy, bacterial growth, biofilm, food-borne pathogens, minimum inhibitory concentrations, rhamnolipids, usnic acid,
• MeSH
• acetylcystein farmakologie   MeSH
• antibakteriální látky farmakologie   MeSH
• antiinfekční látky farmakologie   MeSH
• benzofurany farmakologie   MeSH
• biofilmy účinky léků   MeSH
• buněčné linie MeSH
• Escherichia coli účinky léků   MeSH
• glykolipidy farmakologie   MeSH
• kontaminace potravin prevence a kontrola   MeSH
• lidé MeSH
• Listeria monocytogenes účinky léků   MeSH
• mikrobiální testy citlivosti MeSH
• nemoci přenášené potravou farmakoterapie   mikrobiologie   MeSH
• potravinářská mikrobiologie metody   MeSH
• Salmonella enterica účinky léků   MeSH
• Staphylococcus aureus účinky léků   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• acetylcystein MeSH
• antibakteriální látky MeSH
• antiinfekční látky MeSH
• benzofurany MeSH
• glykolipidy MeSH
• rhamnolipid MeSH Prohlížeč
• usnic acid MeSH Prohlížeč