OBJECTIVES: As a liquid organic fertilizer used in agriculture, digestate is rich in many nutrients (i.e. nitrogen, phosphorus, sulfur, calcium, potassium); their utilization may be however less efficient in soils poor in organic carbon (due to low carbon:nitrogen ratio). In order to solve the disadvantages, digestate enrichment with carbon-rich amendments biochar or humic acids (Humac) was tested. METHODS: Soil variants amended with enriched digestate: digestate + biochar, digestate + Humac, and digestate + combined biochar and humic acids-were compared to control with untreated digestate in their effect on total soil carbon and nitrogen, microbial biomass carbon, soil respiration and soil enzymatic activities in a pot experiment. Yield of the test crop lettuce was also determined for all variants. RESULTS: Soil respiration was the most significantly increased property, positively affected by digestate + Humac. Both digestate + biochar and digestate + Humac significantly increased microbial biomass carbon. Significant negative effect of digestate + biochar (compared to the control digestate) on particular enzyme activities was alleviated by the addition of humic acids. No significant differences among the tested variants were found in the above-ground and root plant biomass. CONCLUSIONS: The tested organic supplements improved the digestate effect on some determined soil properties. We deduced from the results (carbon:nitrogen ratio, microbial biomass and activity) that the assimilation of nutrients by plants increased; however, the most desired positive effect on the yield of crop biomass was not demonstrated. We assume that the digestate enrichment with organic amendments may be more beneficial in a long time-scaled trial.

• MeSH
• biomasa * MeSH
• dřevěné a živočišné uhlí * chemie   farmakologie   MeSH
• dusík * metabolismus   MeSH
• huminové látky * analýza   MeSH
• průmyslová hnojiva MeSH
• půda * chemie   MeSH
• půdní mikrobiologie * MeSH
• uhlík * metabolismus   chemie   MeSH
• Publikační typ
• časopisecké články MeSH
• odvolaná publikace MeSH
• Názvy látek
• biochar MeSH Prohlížeč
• dřevěné a živočišné uhlí * MeSH
• dusík * MeSH
• huminové látky * MeSH
• průmyslová hnojiva MeSH
• půda * MeSH
• uhlík * MeSH

Contamination of soil by copper (Cu) has become a serious problem throughout the world, causing the reduction of agricultural yield and harmful effects on human health by entering the food chain. A glasshouse pot experiment was designed to evaluate the potential use of halloysite as an immobilizing agent in the aided phytostabilization of Cu-contaminated soil, using Festuca rubra L. The content of Cu in plants, i.e., total and extracted by 0.01 M CaCl₂, was determined using the method of spectrophotometry. Cu content in the tested parts of F. rubra differed significantly when halloysite was applied to the soil, as well as with increasing concentrations of Cu. The addition of halloysite significantly increased plant biomass. Cu accumulated in the roots, thereby reducing its toxicity to the aerial parts of the plant. The obtained values of bioconcentration and translocation factors observed for halloysite treatment indicate the effectiveness of using F. rubra in phytostabilization techniques.

• Klíčová slova
• aided phytostabilization, clay minerals, metal contaminated soil, red fescue, risk minimization,
• MeSH
• biodegradace MeSH
• biomasa MeSH
• Festuca růst a vývoj   metabolismus   MeSH
• kořeny rostlin růst a vývoj   metabolismus   MeSH
• látky znečišťující půdu chemie   metabolismus   MeSH
• měď chemie   metabolismus   MeSH
• silikáty hliníku chemie   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• látky znečišťující půdu MeSH
• měď MeSH
• silikáty hliníku MeSH