Dynamic changes in maternal‒zygotic transition (MZT) require complex regulation of zygote formation, maternal transcript decay, embryonic genome activation (EGA), and cell cycle progression. Although these changes are well described, some key regulatory factors are still elusive. Sirtuin-1 (SIRT1), an NAD+-dependent histone deacetylase, is a versatile driver of MZT via its epigenetic and nonepigenetic substrates. This study focused on the dynamics of SIRT1 in early embryos and its contribution to MZT. A conditional SIRT1-deficient knockout mouse model was used, accompanied by porcine and human embryos. Embryos across mammalian species showed the prominent localization of SIRT1 in the nucleus throughout early embryonic development. Accordingly, SIRT1 interacts with histone H4 on lysine K16 (H4K16) in both mouse and human blastocysts. While maternal SIRT1 is dispensable for MZT, at least one allele of embryonic Sirt1 is required for early embryonic development around the time of EGA. This role of SIRT1 is surprisingly mediated via a transcription-independent mode of action.

• Klíčová slova
• Embryo, Embryonic genome activation, Epigenetics, Histone deacetylase, Oocyte, zygote,
• MeSH
• blastocysta metabolismus   MeSH
• embryo savčí metabolismus   MeSH
• embryonální vývoj * genetika   MeSH
• histony metabolismus   MeSH
• lidé MeSH
• myši knockoutované * MeSH
• myši MeSH
• prasata MeSH
• sirtuin 1 * metabolismus   genetika   MeSH
• vývojová regulace genové exprese MeSH
• zvířata MeSH
• zygota * metabolismus   MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• histony MeSH
• SIRT1 protein, human MeSH Prohlížeč
• Sirt1 protein, mouse MeSH Prohlížeč
• sirtuin 1 * MeSH

The nucleolus is an important nucleus sub-organelle found in almost all eukaryotic cells. On the one hand, it is known as a differentiated active site of ribosome biogenesis in somatic cells, but on the other hand, in fully grown oocytes, zygotes, and early embryos (up to the major embryonic genome activation), it is in the form of a particular homogenous and compact structure called a fibrillar sphere. Nowadays, thanks to recent studies, we know many important functions of this, no doubt, interesting membraneless nucleus sub-organelle involved in oocyte maturation, embryonic genome activation, rRNA synthesis, etc. However, many questions are still unexplained and remain a mystery. Our aim is to create a comprehensive overview of the recent knowledge on the fibrillar sphere and envision how this knowledge could be utilized in further research in the field of biotechnology and nucleolotransfer therapy.

• Klíčová slova
• embryo, nucleolotransfer, nucleolus, oocyte, zygote,
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH