The present study investigates the effect of an oxidized nanocrystalline diamond (O-NCD) coating functionalized with bone morphogenetic protein 7 (BMP-7) on human osteoblast maturation and extracellular matrix mineralization in vitro and on new bone formation in vivo. The chemical structure and the morphology of the NCD coating and the adhesion, thickness and morphology of the superimposed BMP-7 layer have also been assessed. The material analysis proved synthesis of a conformal diamond coating with a fine nanostructured morphology on the Ti6Al4V samples. The homogeneous nanostructured layer of BMP-7 on the NCD coating created by a physisorption method was confirmed by AFM. The osteogenic maturation of hFOB 1.19 cells in vitro was only slightly enhanced by the O-NCD coating alone without any increase in the mineralization of the matrix. Functionalization of the coating with BMP-7 resulted in more pronounced cell osteogenic maturation and increased extracellular matrix mineralization. Similar results were obtained in vivo from micro-CT and histological analyses of rabbit distal femurs with screws implanted for 4 or 12 weeks. While the O-NCD-coated implants alone promoted greater thickness of newly-formed bone in direct contact with the implant surface than the bare material, a further increase was induced by BMP-7. It can be therefore concluded that O-NCD coating functionalized with BMP-7 is a promising surface modification of metallic bone implants in order to improve their osseointegration.

• MeSH
• Coated Materials, Biocompatible chemistry   pharmacology   MeSH
• Diamond chemistry   MeSH
• Extracellular Matrix MeSH
• Bone Morphogenetic Protein 7 * pharmacology   MeSH
• Rabbits MeSH
• Osseointegration * MeSH
• Alloys MeSH
• Titanium MeSH
• Animals MeSH
• Check Tag
• Rabbits MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Coated Materials, Biocompatible MeSH
• Diamond MeSH
• Bone Morphogenetic Protein 7 * MeSH
• Alloys MeSH
• Titanium MeSH
• titanium alloy (TiAl6V4) MeSH Browser

A silicalite-1 film (SF) deposited on Ti-6Al-4V alloy was investigated in this study as a promising coating for metallic implants. Two forms of SFs were prepared: as-synthesized SFs (SF-RT), and SFs heated up to 500 °C (SF-500) to remove the excess of template species from the SF surface. The SFs were characterized in detail by X-ray photoelectron spectroscopy (XPS), by Fourier transform infrared spectroscopy (FTIR), by scanning electron microscopy (SEM) and water contact angle measurements (WCA). Two types of bone-derived cells (hFOB 1.19 non-tumor fetal osteoblast cell line and U-2 OS osteosarcoma cell line) were used for a biocompatibility assessment. The initial adhesion of hFOB 1.19 cells, evaluated by cell numbers and cell spreading area, was better supported by SF-500 than by SF-RT. While no increase in cell membrane damage, in ROS generation and in TNF-alpha secretion of bone-derived cells grown on both SFs was found, gamma H2AX staining revealed an elevated DNA damage response of U-2 OS cells grown on heat-treated samples (SF-500). This study also discusses differences between osteosarcoma cell lines and non-tumor osteoblastic cells, stressing the importance of choosing the right cell type model.

• MeSH
• Biocompatible Materials chemistry   MeSH
• Cell Membrane drug effects   MeSH
• Cell Line MeSH
• Cytotoxins chemistry   pharmacology   MeSH
• Photoelectron Spectroscopy methods   MeSH
• Humans MeSH
• Microscopy, Electron, Scanning methods   MeSH
• Cell Line, Tumor MeSH
• Osteoblasts drug effects   MeSH
• Osteocytes drug effects   MeSH
• Surface Properties drug effects   MeSH
• Cell Proliferation drug effects   MeSH
• Alloys MeSH
• Materials Testing methods   MeSH
• Titanium chemistry   MeSH
• Hot Temperature MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Biocompatible Materials MeSH
• Cytotoxins MeSH
• Alloys MeSH
• Titanium MeSH
• titanium alloy (TiAl6V4) MeSH Browser