Poly(2-oxazoline) is a promising new class of polymeric materials due to their antibiofouling properties and good biocompatibility. Poly(2-oxazoline) coatings can be deposited on different substrates via plasma polymerization, which can be more advantageous than other coating methods. The aim of this study is to deposit poly(2-oxazoline) coatings using a surface dielectric barrier discharge burning in nitrogen at atmospheric pressure using 2-methyl-2-oxazoline and 2-ethyl-2-oxazoline vapours as monomers and compare the film properties. For the comparison, the antibacterial and cytocompatibility tests were peformed according to ISO norms. The antibacterial tests showed that all the deposited films were highly active against Staphylococcus aureus and Escherichia coli bacteria. The chemical composition of the films was studied using FTIR and XPS, and the film surface's properties were studied using AFM and surface energy measurement. The cytocompatibility tests showed good cytocompatibility of all the deposited films. However, the films deposited from 2-methyl-2-oxazoline exhibit better cytocompatibility. This difference can be explained by the different chemical compositions and surface morphologies of the films deposited from different monomers.

• Klíčová slova
• antibiofouling, plasma polymer, poly(2-oxazoline),
• MeSH
• antibakteriální látky * farmakologie   MeSH
• Escherichia coli MeSH
• oxazoly * farmakologie   chemie   MeSH
• polymerizace MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• 2-ethyl-2-oxazoline MeSH Prohlížeč
• 2-methyl-2-oxazoline MeSH Prohlížeč
• antibakteriální látky * MeSH
• oxazoly * MeSH

In the technological processes requiring mild treatment, such as soft materials processing or medical applications, an important role is played by non-equilibrium plasma reactors with dielectric barrier discharge (DBD), that when generated in noble gases allows for the effective treatment of biological material at a low temperature. The aim of this study is to determine the operating parameters of an atmospheric pressure, radio-frequency DBD plasma jet reactor for the precise treatment of biological materials. The tested parameters were the shape of the discharge (its length and volume), current and voltage signals, as well as the power consumed by the reactor for various composition and flow rates of the working gas. To determine the applicability in medicine, the temperature, pH, concentrations of H2O2, NO2- and NO3- and Escherichia coli log reduction in the plasma treated liquids were determined. The obtained results show that for certain operating parameters, a narrow shape of plasma stream can generate significant amounts of H2O2, allowing for the mild decontamination of bacteria at a relatively low power of the system, safe for the treatment of biological materials.

• Klíčová slova
• Escherichia coli, atmospheric pressure plasma, biological materials, dielectric barrier discharge, plasma jet, plasma treatment of liquid, reactive oxygen and nitrogen species,
• Publikační typ
• časopisecké články MeSH

Antibacterial coatings on biomedical instruments are of great interest because they can suppress bacterial colonization on these instruments. In this study, antibacterial polymeric thin coatings were deposited on teflon substrates using atmospheric pressure plasma polymerization from a propane-butane mixture. The plasma polymerization was performed by means of surface dielectric barrier discharge burning in nitrogen at atmospheric pressure. The chemical composition of plasma polymerized propane-butane films was studied by energy-dispersive X-ray spectroscopy (EDX) and FTIR. The film surface properties were studied by SEM and by surface energy measurement. The EDX analysis showed that the films consisted of carbon, nitrogen and oxygen from ambient air. The FTIR analysis confirmed, in particular, the presence of alkyl, nitrile, acetylene, imide and amine groups. The deposited films were hydrophilic with a water contact angle in the range of 13-23°. The thin film deposited samples were highly active against both S. aureus and E. coli strains in general. On the other hand, the films were cytocompatible, reaching more than 80% of the cell viability threshold compared to reference polystyrene tissue.

• Klíčová slova
• antibacterial thin films, plasma polymer, propane–butane mixture,
• MeSH
• antibakteriální látky farmakologie   MeSH
• atmosférický tlak MeSH
• butany MeSH
• dusík MeSH
• Escherichia coli MeSH
• propan * MeSH
• Staphylococcus aureus * MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antibakteriální látky MeSH
• butany MeSH
• dusík MeSH
• propan * MeSH