Gestational diabetes mellitus (GDM) is a complication in pregnancy, but studies focused on the steroidome in patients with GDM are not available in the public domain. This article evaluates the steroidome in GDM+ and GDM- women and its changes from 24 weeks (± of gestation) to labor. The study included GDM+ (n = 44) and GDM- women (n = 33), in weeks 24-28, 30-36 of gestation and at labor and mixed umbilical blood after delivery. Steroidomic data (101 steroids quantified by GC-MS/MS) support the concept that the increasing diabetogenic effects with the approaching term are associated with mounting progesterone levels. The GDM+ group showed lower levels of testosterone (due to reduced AKR1C3 activity), estradiol (due to a shift from the HSD17B1 towards HSD17B2 activity), 7-oxygenated androgens (competing with cortisone for HSD11B1 and shifting the balance from diabetogenic cortisol towards the inactive cortisone), reduced activities of SRD5As, and CYP17A1 in the hydroxylase but higher CYP17A1 activity in the lyase step. With the approaching term, the authors found rising activities of CYP3A7, AKR1C1, CYP17A1 in its hydroxylase step, but a decline in its lyase step, rising conjugation of neuroinhibitory and pregnancy-stabilizing steroids and weakening AKR1D1 activity.

• Klíčová slova
• gas chromatography-tandem mass spectrometry, gestational age, gestational diabetes mellitus, immunoprotective steroids, maternal blood, mixed cord blood, neuroactive steroids, steroidome,
• MeSH
• 20-hydroxysteroid dehydrogenasy metabolismus   MeSH
• chromatografie plynová MeSH
• cytochrom P-450 CYP3A metabolismus   MeSH
• druhý trimestr těhotenství metabolismus   MeSH
• gestační diabetes metabolismus   MeSH
• lidé MeSH
• metabolomika metody   MeSH
• oxidoreduktasy metabolismus   MeSH
• steroid-17-alfa-hydroxylasa metabolismus   MeSH
• steroidy analýza   MeSH
• tandemová hmotnostní spektrometrie MeSH
• těhotenství MeSH
• Check Tag
• lidé MeSH
• těhotenství MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• 20-hydroxysteroid dehydrogenasy MeSH
• 3 alpha-beta, 20 beta-hydroxysteroid dehydrogenase MeSH Prohlížeč
• 3-oxo-5 beta-steroid delta 4-dehydrogenase MeSH Prohlížeč
• CYP17A1 protein, human MeSH Prohlížeč
• CYP3A7 protein, human MeSH Prohlížeč
• cytochrom P-450 CYP3A MeSH
• oxidoreduktasy MeSH
• steroid-17-alfa-hydroxylasa MeSH
• steroidy MeSH