The analysis of dietary supplements is far less regulated than pharmaceuticals, leading to potential quality issues. Considering their positive effect, many athletes consume supplements containing L-histidine and β-alanine. A new microfluidic method for the determination of L-histidine and β-alanine in dietary supplement formulations has been developed. For the first time, capacitively coupled contactless conductivity detection was employed for the microchip electrophoresis of amino acids in real samples. A linear relationship between detector response and concentration was observed in the range of 10-100 µmol L-1 for L-histidine (R2 = 0.9968) and β-alanine (R2 = 0.9954), while achieved limits of detection (3 × S/N ratio) were 4.2 µmol L-1 and 5.2 µmol L-1, respectively. The accuracy of the method was confirmed using recovery experiments as well as CE-UV-VIS and HPLC-UV-VIS techniques. The developed method allows unambiguous identification of amino acids in native form without chemical derivatization and with the possibility of simultaneous analysis of amino acids with metal cations.

• Klíčová slova
• l-Histidine, Amino acids, Capacitively coupled contactless conductivity detection, Dietary supplements, Microchip electrophoresis, β-Alanine,
• MeSH
• beta-alanin * analýza   chemie   MeSH
• elektrická vodivost * MeSH
• elektroforéza mikročipová * metody   MeSH
• histidin * analýza   chemie   MeSH
• limita detekce MeSH
• potravní doplňky * analýza   MeSH
• sklo chemie   MeSH
• technologie zelené chemie metody   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• beta-alanin * MeSH
• histidin * MeSH

Diabetic foot is a serious late complication frequently caused by infection and ischaemia. Both require prompt and aggressive treatment to avoid lower limb amputation. The effectiveness of peripheral arterial disease therapy can be easily verified using triplex ultrasound, ankle-brachial/toe-brachial index examination, or transcutaneous oxygen pressure. However, the success of infection treatment is difficult to establish in patients with diabetic foot. Intravenous systemic antibiotics are recommended for the treatment of infectious complications in patients with moderate or serious stages of infection. Antibiotic therapy should be initiated promptly and aggressively to achieve sufficient serum and peripheral antibiotic concentrations. Antibiotic serum levels are easily evaluated by pharmacokinetic assessment. However, antibiotic concentrations in peripheral tissues, especially in diabetic foot, are not routinely detectable. This review describes microdialysis techniques that have shown promise in determining antibiotic levels in the surroundings of diabetic foot lesions.

• Klíčová slova
• antibiotic (ATB), capillary electrophoresis (CE), diabetic foot (DF), diabetic foot infection (DFI), microdialysis (MD),
• MeSH
• amputace MeSH
• antibakteriální látky terapeutické užití   MeSH
• diabetes mellitus * farmakoterapie   MeSH
• diabetická noha * diagnóza   farmakoterapie   MeSH
• dolní končetina patologie   MeSH
• lidé MeSH
• mikrodialýza škodlivé účinky   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH
• Názvy látek
• antibakteriální látky MeSH