Nejvíce citovaný článek - PubMed ID 4998300
Use of mutagens in the improvement of production strains of microorganisms
The production of epsilon-pyrromycinone glycosides in Streptomyces galilaeus increased 12-fold, with respect to the wild strain, as a result of a sequential procedure including both natural selection and treatment with mutagens (nitrous acid, UV light and gamma-irradiation). Nitrous acid exhibited the highest mutagenic effect, both in increasing the productivity and in inducing blocked mutants. A mutant strain blocked in the biosynthesis of glycosides and accumulating free epsilon-pyrromycinone as the principal metabolite was obtained.
- MeSH
- anthrachinony biosyntéza MeSH
- glykosidy biosyntéza MeSH
- kyselina dusitá farmakologie MeSH
- mutace účinky léků účinky záření MeSH
- protinádorová antibiotika biosyntéza MeSH
- Streptomyces účinky léků genetika metabolismus účinky záření MeSH
- ultrafialové záření MeSH
- záření gama MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- anthrachinony MeSH
- glykosidy MeSH
- kyselina dusitá MeSH
- protinádorová antibiotika MeSH
Variability in the production of nogalamycin by Streptomyces nogalater var. nogalater was followed in untreated and mutagenized populations of the standard strain NRRL 3035 and its spontaneous variant K-18 using the method of agar blocks with subsequent tests under submerged conditions. In both strains the most active variants were obtained by natural selection without mutagenic treatment; in this way productivity increased by 108% after two selection steps. Treatment with UV-radiation did not yield variants with a highly increased activity. Gamma-radiation extended the variability but, at the same, substantially increased the number of non-producing and low-producing isolates. Relatively high yields of (+)-variants were obtained after treatment with nitrous acid but their activity did not reach that observed in the most active spontaneous variants.
- MeSH
- Bacillus subtilis MeSH
- biotest MeSH
- dusitany farmakologie MeSH
- genetická variace * MeSH
- kyselina dusitá farmakologie MeSH
- mithramycin analýza biosyntéza MeSH
- mutageny MeSH
- nitrosoguanidiny MeSH
- radiační genetika MeSH
- Streptomyces účinky léků metabolismus účinky záření MeSH
- ultrafialové záření * MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- dusitany MeSH
- kyselina dusitá MeSH
- mithramycin MeSH
- mutageny MeSH
- nitrosoguanidiny MeSH
