The process of isolation and purification of malate dehydrogenase (decarboxylating) (EC 1.1.1.40) from the mycelium of the actinomycete Streptomyces aureofaciens has been worked out. The enzyme was purified 35 fold. The kinetic characters of the purified enzyme are very similar to the figures for malate dehydrogenase (decarboxylating) from other sources. Km for L-malate = 2.1 X 10(-3)M, Km for NADP = 4.6 X 10(-5)M (at pH 7.4). The reaction requires metal divalent ions, Mn2+ being more effective than Mg2+. The enzyme reaches its maximal activity at pH 8.75.

• MeSH
• Chemical Fractionation MeSH
• Magnesium pharmacology   MeSH
• Hydrogen-Ion Concentration MeSH
• Malate Dehydrogenase * isolation & purification   metabolism   MeSH
• Malates metabolism   MeSH
• Manganese pharmacology   MeSH
• Pyruvates biosynthesis   MeSH
• Ammonium Sulfate MeSH
• Streptomyces aureofaciens enzymology   metabolism   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Magnesium MeSH
• Malate Dehydrogenase * MeSH
• Malates MeSH
• Manganese MeSH
• Pyruvates MeSH
• Ammonium Sulfate MeSH

• MeSH
• Anthraquinones metabolism   MeSH
• Time Factors MeSH
• Chromatography, Thin Layer MeSH
• Fermentation MeSH
• Glucosides biosynthesis   MeSH
• Glycosides biosynthesis   MeSH
• Hydrogen-Ion Concentration MeSH
• Monosaccharides metabolism   MeSH
• Mutation MeSH
• Oligosaccharides metabolism   MeSH
• Buffers MeSH
• Sucrose metabolism   MeSH
• Spectrophotometry MeSH
• Streptomyces aureofaciens metabolism   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Anthraquinones MeSH
• Glucosides MeSH
• Glycosides MeSH
• Monosaccharides MeSH
• Oligosaccharides MeSH
• Buffers MeSH
• Sucrose MeSH

• MeSH
• Anthracenes biosynthesis   metabolism   MeSH
• Chemical Phenomena MeSH
• Chemistry MeSH
• Chlortetracycline biosynthesis   MeSH
• Decarboxylation MeSH
• Genes * MeSH
• Glucose metabolism   MeSH
• Coenzyme A biosynthesis   metabolism   MeSH
• Genetics, Microbial * MeSH
• Streptomyces metabolism   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Anthracenes MeSH
• Chlortetracycline MeSH
• Glucose MeSH
• Coenzyme A MeSH

• MeSH
• Carbon Isotopes MeSH
• Ligases biosynthesis   MeSH
• Ovalbumin pharmacology   MeSH
• Streptomyces drug effects   enzymology   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Carbon Isotopes MeSH
• Ligases MeSH
• Ovalbumin MeSH

• MeSH
• Acetates metabolism   MeSH
• Fermentation * MeSH
• Carbon Isotopes MeSH
• Fatty Acids biosynthesis   metabolism   MeSH
• Streptomyces metabolism   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Acetates MeSH
• Carbon Isotopes MeSH
• Fatty Acids MeSH

• MeSH
• Acetates pharmacology   MeSH
• Chlortetracycline biosynthesis   MeSH
• Enzyme Repression MeSH
• Glucose metabolism   MeSH
• Carbon Isotopes MeSH
• Dicarboxylic Acids metabolism   MeSH
• Malate Dehydrogenase metabolism   MeSH
• Streptomyces enzymology   metabolism   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Acetates MeSH
• Chlortetracycline MeSH
• Glucose MeSH
• Carbon Isotopes MeSH
• Dicarboxylic Acids MeSH
• Malate Dehydrogenase MeSH

• MeSH
• Adenine Nucleotides pharmacology   MeSH
• Adenosine Triphosphate pharmacology   MeSH
• Citrates MeSH
• Lyases analysis   antagonists & inhibitors   metabolism   MeSH
• Nucleotides pharmacology   MeSH
• Spectrophotometry MeSH
• Streptomyces drug effects   enzymology   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Adenine Nucleotides MeSH
• Adenosine Triphosphate MeSH
• Citrates MeSH
• Lyases MeSH
• Nucleotides MeSH

• MeSH
• Chlortetracycline biosynthesis   MeSH
• Chromatography, Gas MeSH
• Lipids analysis   MeSH
• Fatty Acids analysis   biosynthesis   MeSH
• Streptomyces analysis   metabolism   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Chlortetracycline MeSH
• Lipids MeSH
• Fatty Acids MeSH