Mycelium of Streptomyces cinnamonensis mutant strains cultivated in a synthetic medium with glycine produced protoplasts after lysis of cell walls with lysozyme. The protoplast yield was up to 95%. The protoplasts could revert and mycelial forms were thus regenerated. In a sucrose-containing medium the protoplasts stored at 4 degrees C were stable for 2 d.

• MeSH
• Glycine MeSH
• Culture Media MeSH
• Muramidase MeSH
• Mutation MeSH
• Mycology methods   MeSH
• Protoplasts cytology   physiology   MeSH
• Streptomyces cytology   genetics   physiology   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Glycine MeSH
• Culture Media MeSH
• Muramidase MeSH

Vitamins added to submerged Streptomyces cinnamonensis cultures stimulated the production of monensins. Vitamins B2, B3, B5 and B12 enhanced the production by about 50%, vitamins B1 and B6 by 100%. The addition of biotin in optimal concentration resulted in more than 3-fold increase in total production.

• MeSH
• Biotin pharmacology   MeSH
• Culture Media MeSH
• Pantothenic Acid pharmacology   MeSH
• Monensin biosynthesis   MeSH
• Niacin pharmacology   MeSH
• Pyridoxine pharmacology   MeSH
• Riboflavin pharmacology   MeSH
• Streptomyces drug effects   metabolism   MeSH
• Thiamine pharmacology   MeSH
• Vitamin B 12 pharmacology   MeSH
• Vitamin B Complex pharmacology   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Biotin MeSH
• Culture Media MeSH
• Pantothenic Acid MeSH
• Monensin MeSH
• Niacin MeSH
• Pyridoxine MeSH
• Riboflavin MeSH
• Thiamine MeSH
• Vitamin B 12 MeSH
• Vitamin B Complex MeSH

Variants resistant to propionate were prepared from a mutant strain of Streptomyces cinnamonensis producing predominantly monensin A. Using selected resistants the production of monensins (in media with higher concentrations of propionate) was examined. Stimulation of monensin synthesis by propionate was observed with 70% of the resistants studied. Propionate did not influence the ratio between monensin A and B production.

• MeSH
• Drug Resistance, Microbial MeSH
• Monensin biosynthesis   MeSH
• Mutation MeSH
• Propionates pharmacology   MeSH
• Streptomyces metabolism   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Monensin MeSH
• Propionates MeSH

Precursors of monensins (acetate, propionate, butyrate, isobutyrate) affect the total production and the relative proportion of monensins A and B. Addition of propionate into the fermentation medium causes a prevalence of monensin B whereas butyrate and isobutyrate stimulate the production of monensin A and suppress the production of monensin B.

• MeSH
• Acetates pharmacology   MeSH
• Butyrates pharmacology   MeSH
• Furans biosynthesis   MeSH
• Isobutyrates MeSH
• Butyric Acid MeSH
• Acetic Acid MeSH
• Monensin biosynthesis   MeSH
• Propionates pharmacology   MeSH
• Streptomyces metabolism   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Acetates MeSH
• Butyrates MeSH
• Furans MeSH
• Isobutyrates MeSH
• isobutyric acid MeSH Browser
• Butyric Acid MeSH
• Acetic Acid MeSH
• Monensin MeSH
• Propionates MeSH
• propionic acid MeSH Browser

A diffusion plate method for the assay of the activity of lipases in large series of samples was worked out. When using this method the maximum deviation was found to be +/- 3.8%.

• MeSH
• Candida enzymology   MeSH
• Diffusion MeSH
• Kinetics MeSH
• Lipase analysis   MeSH
• Methods MeSH
• Pancreas enzymology   MeSH
• Swine MeSH
• Streptomyces enzymology   MeSH
• Animals MeSH
• Check Tag
• Animals MeSH
• Publication type
• Journal Article MeSH
• Comparative Study MeSH
• Names of Substances
• Lipase MeSH