Most cited article - PubMed ID 7813882
Pulsed-field gel electrophoresis of the genomic restriction fragments of coagulase-negative staphylococci
Bacterial species of the genus Staphylococcus known as important human and animal pathogens are the cause of a number of severe infectious diseases. Apart from the major pathogen Staphylococcus aureus, other species until recently considered to be nonpathogenic may also be involved in serious infections. Rapid and accurate identification of the disease-causing agent is therefore prerequisite for disease control and epidemiological surveillance. Modern methods for identification and typing of bacterial species are based on genome analysis and have many advantages compared to phenotypic methods. The genotypic methods currently used in molecular diagnostics of staphylococcal species, particularly of S. aureus, are reviewed. Attention is also paid to new molecular methods with the highest discriminatory power. Efforts made to achieve interlaboratory reproducibility of diagnostic methods are presented.
- MeSH
- Genotype MeSH
- Humans MeSH
- Polymerase Chain Reaction MeSH
- Methicillin Resistance MeSH
- Sequence Analysis, DNA MeSH
- Staphylococcus classification genetics isolation & purification MeSH
- Bacterial Typing Techniques MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Review MeSH