Seventy-eight staphylococcal strains were isolated from surgical-site, blood-stream and other hospital-acquired infections. Eighteen isolates were determined as methicillin (MET)-resistant S. aureus (MRSA), while the remaining were MET-resistant coagulase-negative staphylococci (CoNS). Fifty percent of CoNS strains were multiresistant, while 10 % of isolates were resistant only to beta-lactams. Clinical isolates of CoNS were generally more resistant to antimicrobial agents than S. aureus strains. Thirty-nine % of S. aureus strains were resistant only to beta-lactams. None of the MRSA strains carried ileS-2 gene; this gene was found in two strains of S. epidermidis.

• MeSH
• antibakteriální látky farmakologie   MeSH
• bakteriální léková rezistence * MeSH
• bakteriální proteiny genetika   MeSH
• isoleucin-tRNA-ligasa genetika   MeSH
• koagulasa metabolismus   MeSH
• lidé MeSH
• methicilin rezistentní Staphylococcus aureus účinky léků   enzymologie   genetika   MeSH
• mupirocin farmakologie   MeSH
• rezistence na methicilin MeSH
• stafylokokové infekce farmakoterapie   mikrobiologie   MeSH
• Staphylococcus účinky léků   enzymologie   genetika   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antibakteriální látky MeSH
• bakteriální proteiny MeSH
• isoleucin-tRNA-ligasa MeSH
• koagulasa MeSH
• mupirocin MeSH

Bacterial species of the genus Staphylococcus known as important human and animal pathogens are the cause of a number of severe infectious diseases. Apart from the major pathogen Staphylococcus aureus, other species until recently considered to be nonpathogenic may also be involved in serious infections. Rapid and accurate identification of the disease-causing agent is therefore prerequisite for disease control and epidemiological surveillance. Modern methods for identification and typing of bacterial species are based on genome analysis and have many advantages compared to phenotypic methods. The genotypic methods currently used in molecular diagnostics of staphylococcal species, particularly of S. aureus, are reviewed. Attention is also paid to new molecular methods with the highest discriminatory power. Efforts made to achieve interlaboratory reproducibility of diagnostic methods are presented.

• MeSH
• genotyp MeSH
• lidé MeSH
• polymerázová řetězová reakce MeSH
• rezistence na methicilin MeSH
• sekvenční analýza DNA MeSH
• Staphylococcus klasifikace   genetika   izolace a purifikace   MeSH
• techniky typizace bakterií MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH