Three fluorescent probes, tetramethyl rhodamine ethyl ester (TMRE), 3,3'-dipropylthiacarbocyanine iodide (diS-C3(3)) and 3,3'-dipropyloxacarbocyanine iodide (diO-C3(3)), were tested for their suitability as fluorescent indicators of membrane potential in Saccharomyces cerevisiae in studies performed by flow cytometry. For all these dyes the intensity of fluorescence of stained cells increased with probe concentration in the range of 60-3000 nmol/L. The optimum staining period was 15-20 min for diS-C3(3). Depolarization of cells by increased extracellular potassium level and by valinomycin elicited with all probes a drop in fluorescence intensity. In some yeast batches this depolarization was accompanied by a separation of subpopulations with different fluorescence properties.

• MeSH
• Artifacts MeSH
• Benzothiazoles MeSH
• Time Factors MeSH
• Fluorescence MeSH
• Fluorescent Dyes * MeSH
• Fluorometry * MeSH
• Indicators and Reagents MeSH
• Carbocyanines * MeSH
• Membrane Potentials * MeSH
• Organometallic Compounds MeSH
• Flow Cytometry methods   MeSH
• Pyridinium Compounds analysis   metabolism   MeSH
• Scattering, Radiation MeSH
• Saccharomyces cerevisiae physiology   MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• 1-(3-sulfonatopropyl)-4-(beta)(2-(di-n-butylamino)-6-naphthylvinyl)pyridinium betaine MeSH Browser
• 3,3'-dipropyl-2,2'-thiadicarbocyanine MeSH Browser
• 3,3'-dipropyloxadicarbocyanine MeSH Browser
• 3,3'-dipropylthiacarbocyanine MeSH Browser
• Benzothiazoles MeSH
• Fluorescent Dyes * MeSH
• Indicators and Reagents MeSH
• Carbocyanines * MeSH
• Organometallic Compounds MeSH
• Pyridinium Compounds MeSH
• tetramethyl rhodamine ethyl ester MeSH Browser

• MeSH
• Genetic Techniques MeSH
• Hybridization, Genetic MeSH
• Killer Factors, Yeast MeSH
• Mutation MeSH
• Mycotoxins biosynthesis   genetics   pharmacology   MeSH
• Industry MeSH
• Saccharomyces cerevisiae Proteins MeSH
• Saccharomyces cerevisiae drug effects   genetics   metabolism   MeSH
• Transformation, Genetic MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Review MeSH
• Names of Substances
• KHR1 protein, S cerevisiae MeSH Browser
• Killer Factors, Yeast MeSH
• Mycotoxins MeSH
• Saccharomyces cerevisiae Proteins MeSH