A total of 5 Bifidobacterium spp. isolated from pig and children' feces and 6 Lactobacillus spp. from chicken feces were examined for expression of aggregation, cell surface hydrophobicity (CSH) and adherence to intestinal mucin. Co-aggregation activity was seen in 3 strains of auto-aggregative bifidobacteria and 4 auto-aggregative strains of Lactobacillus spp. with 2 enterohemorrhagic E. coli (O157). CSH correlated with Lactobacillus auto-aggregating activity and adherence to mucin but the correlation between Bifidobacterium adherence to mucin and CSH was not confirmed.

• MeSH
• bakteriální adheze * MeSH
• Bifidobacterium izolace a purifikace   fyziologie   MeSH
• Escherichia coli O157 fyziologie   MeSH
• feces mikrobiologie   MeSH
• hydrofobní a hydrofilní interakce MeSH
• kur domácí mikrobiologie   MeSH
• Lactobacillus izolace a purifikace   fyziologie   MeSH
• lidé MeSH
• muciny metabolismus   MeSH
• prasata mikrobiologie   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• muciny MeSH

The impact of postantibiotic effect (PAE) of carbapenems (imipenem, meropenem) on the metabolism (biosynthesis of macromolecules, respiration), cell-surface hydrophobicity and motility of a clinical isolate of Enterobacter cloacae was examined. The metabolism was evaluated after 16 h and after 1 d of cultivation using 2x and 4x minimum inhibitory concentrations (MIC) of both antibiotics for the induction of PAE. Imipenem at 4 x MIC did not induce PAE. After a 16-h cultivation (in the postantibiotic phase of both carbapenems), inhibition of nucleosynthesis and protein synthesis was found; after a 1-d cultivation, during regrowth stimulation of mainly 14C-leucine incorporation was found. The presence of the exogenous intermediates of citrate cycle, viz. 2-oxoglutarate, increased the respiratory activity of the cells. The cell-surface hydrophobicity (evaluated by three methods--bacterial adhesion to hydrocarbon, nitrocellulose-filter test and salt-aggregation test) decreased after PAE of both carbapenems; meropenem was more effective. Motility (an important virulence factor) was inhibited in the postantibiotic phase of both carbapenems; the 4 x MIC caused a higher inhibition.

• MeSH
• časové faktory MeSH
• Enterobacter cloacae chemie   účinky léků   fyziologie   MeSH
• hydrofobní a hydrofilní interakce MeSH
• imipenem farmakologie   MeSH
• karbapenemy farmakologie   MeSH
• lidé MeSH
• meropenem MeSH
• pohyb účinky léků   MeSH
• thienamyciny farmakologie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH
• Názvy látek
• imipenem MeSH
• karbapenemy MeSH
• meropenem MeSH
• thienamyciny MeSH

Nitroxoline (5-nitro-8-quinolinol; NIQ) at subinhibitory concentrations (sub-MIC) decreased the adherence of uropathogenic Escherichia coli to catheter surface and significantly enhanced cell surface hydrophobicity. The surface hydrophobicity increased in the presence of sub-MIC of NIQ and also in an excess of Mg2+. The effect of NIQ on the cell surface was not related to the bacteriostatic effect of this agent. The increase in nitrogen and decrease in phosphate content in the cell surface was found in the presence of NIQ. NIQ did not inhibit the expression of fimbriae.

• MeSH
• bakteriální adheze účinky léků   MeSH
• buněčná membrána chemie   účinky léků   ultrastruktura   MeSH
• elektronová mikroskopie MeSH
• Escherichia coli chemie   účinky léků   patogenita   MeSH
• infekce močového ústrojí farmakoterapie   etiologie   MeSH
• infekce vyvolané Escherichia coli farmakoterapie   etiologie   MeSH
• katetrizace močového měchýře škodlivé účinky   MeSH
• lidé MeSH
• nitrochinoliny aplikace a dávkování   farmakologie   MeSH
• povrchové vlastnosti MeSH
• techniky in vitro MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• nitrochinoliny MeSH
• nitroxoline MeSH Prohlížeč

The antimicrobial and morphogenetic effects of fourteen newly synthesized 2-substituted derivatives of quinoline-4-carboxylic acid and quinoline-4-carboxamide were studied using G+ and G- bacteria, yeasts and filamentous fungi. The highest antimicrobial effects were found with substituted quinoline-4-carboxylic acid derivatives. Quinoline-4-carboxamides only weakly influenced the growth of the tested microorganisms. Some derivatives of quinoline-4-carboxylic acid elicited profound changes in the morphology of hyphal tips of Botrytis cinerea, mainly their branching and the release of the cytoplasmic content. Quinoline derivatives, which elicited morphological changes, increased also the permeability of the plasmalemma of plant cells.

• MeSH
• anthokyaniny metabolismus   MeSH
• antibakteriální látky MeSH
• antiinfekční látky farmakologie   MeSH
• Bacteria účinky léků   MeSH
• Botrytis cytologie   účinky léků   MeSH
• Chenopodiaceae účinky léků   metabolismus   MeSH
• chinoliny chemie   farmakologie   MeSH
• houby účinky léků   MeSH
• mikrobiální testy citlivosti MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• anthokyaniny MeSH
• antibakteriální látky MeSH
• antiinfekční látky MeSH
• chinoliny MeSH
• quinoline-4-carboxylic acid MeSH Prohlížeč

The impact of postantibiotic effect (PAE) of aminoglycosides (gentamicin, netilmicin) on cell-surface hydrophobicity and motility of a clinical isolate Serratia marcescens was evaluated. For the induction of PAE 2x and 4xMIC concentrations of both antibiotics were used. Gentamicin and netilmicin induced a PAE of similar duration after 2xMIC concentration (2.7 and 2.8 h, respectively). Both aminoglycosides demonstrated concentration-dependent PAE. At a concentration of 4xMIC they produced PAEs of 5.9 and 8.2 h, respectively. The evaluation of hydrophobic properties of S. marcescens after affecting PAE showed that both aminoglycosides inhibited adherence to xylene. This inhibition was also concentration-dependent. More expressive was netilmicin which inhibited the adhesion by 70.5% at 2xMIC and by 85.2% at 4xMIC. Netilmicin inhibited also the adhesion to nitrocellulose filter by 34.7% at 4xMIC. Exposure of the bacterial cells to suprainhibitory concentrations of both aminoglycosides resulted only in moderate inhibition of motility of strain tested compared to the unexposed cells.

• MeSH
• antibakteriální látky farmakologie   MeSH
• bakteriální adheze účinky léků   MeSH
• gentamiciny farmakologie   MeSH
• lidé MeSH
• netilmicin farmakologie   MeSH
• pohyb MeSH
• Serratia marcescens účinky léků   fyziologie   MeSH
• vztah mezi dávkou a účinkem léčiva MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antibakteriální látky MeSH
• gentamiciny MeSH
• netilmicin MeSH