Interleukin-17A (IL-17) is a pro-inflammatory cytokine involved in the immune response to many pathogens playing also a role in certain chronic and autoimmune diseases. The presented study focused on the early postnatal development of IL-17 producing cells in swine. In agreement with previous studies, αβ T-helper (CD3+CD4+) and γδ T (CD3+TCRγδ+) cells were found to be the major producers of IL-17. In newborn conventional piglets, αβ T-helper cells positive for IL-17 were almost undetectable, but their frequency increased markedly with age in all issues examined, i.e., blood, spleen, and mesenteric lymph nodes (MLN). Additional analyses of CD8 and CD27 expression showed that the main αβ T-helper producers of IL-17 has CD8+CD27- phenotype in all tissues. IL-17 positive CD8+CD27+ αβ T-helper subpopulation was found only in blood and spleen. The production of IL17 in CD8-CD27+ αβ T-helper cells was always minor. In contrast, γδ T cells positive for IL-17 did not show a similar age-dependent increase in blood and spleen, whereas they increased in MLN. Because of the age-dependent increase in conventional animals, we included a comparison with germ-free piglets to show that the increase in IL-17 positive cells was clearly depended on the presence of the microbiota as the production in germ-free animals was negligible without any age-dependent increase.

• Klíčová slova
• T cells, germ-free, interleukin-17, postnatal, swine,
• MeSH
• interleukin-17 * metabolismus   MeSH
• mikrobiota * MeSH
• prasata MeSH
• receptory antigenů T-buněk gama-delta metabolismus   MeSH
• T-lymfocyty - podskupiny MeSH
• výzkumná zpráva MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• interleukin-17 * MeSH
• receptory antigenů T-buněk gama-delta MeSH

Cellular and humoral aspects of the immune response develop sequentially in the fetus. During the ontogeny, the pluripotent stem cells emerge and differentiate into all hematopoietic lineages. Basic questions including the identification of the first lympho-hematopoietic sites, the origin of T and B lymphocytes, the development of different subpopulations of alphabeta T, gammadelta T and B lymphocytes as well as development of innate immunity and the acquisition of full immunological capacities are discussed here for swine and compared with other species. The description of related topics such as fertilization, morphogenesis, maternal-fetal-neonatal physiology and early neonatal development are also discussed.

• MeSH
• B-lymfocyty cytologie   imunologie   metabolismus   MeSH
• buněčná imunita MeSH
• embryo savčí imunologie   MeSH
• hematopoetické kmenové buňky cytologie   imunologie   MeSH
• hematopoéza imunologie   MeSH
• imunitní systém embryologie   imunologie   MeSH
• lymfopoéza MeSH
• maternofetální výměna látek imunologie   MeSH
• morfogeneze imunologie   MeSH
• placentace imunologie   MeSH
• prasata embryologie   imunologie   virologie   MeSH
• přirozená imunita MeSH
• T-lymfocyty cytologie   imunologie   metabolismus   MeSH
• těhotenství MeSH
• zvířata MeSH
• Check Tag
• těhotenství MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH

In this report, we describe 12 subpopulations of porcine gammadelta thymocytes based on their expression of CD1, CD2, CD4, CD8- isoforms and CD45RC. Our data suggest that gammadelta thymocytes can be divided into two major families: (a) one large family of CD4-gammadelta thymocytes that could be further subdivided according to the CD2/CD8alphaalpha phenotype and (b) a small family of CD4+ gammadelta thymocytes bearing CD8alphabeta and possessing certain unusual features in comparison with other gammadelta thymocytes. Maturation of gammadelta thymocytes within the CD4- family begins with proliferation of the CD2+ CD8- CD1+ CD45RC- gammadelta common precursor. This developmental stage is followed by diversification into the CD2+ CD8alphaalpha+, CD2+ CD8- and CD2- CD8- subsets. Their further maturation is accompanied by a loss of expression of CD1 and by increased expression of CD45RC. Therefore, individual subsets develop from CD1+ CD45RC- through CD1- CD45RC- into CD1- CD45RC+ cells. On the other hand, gammadelta thymocytes within the CD4+ family bear exclusively CD8alphabeta, always express CD1, but may coexpress CD45RC. These cells have no counterpart in the periphery. Our observations suggest that all peripheral CD8+ gammadelta T cells express CD8alphaalpha and that two subsets of these cells differing in major histocompatibility complex II expression, occur. We propose that one subset acquires CD8alphaalpha in the thymus while the second acquires CD8alphaalpha as a result of stimulation in the periphery.

• MeSH
• antigeny CD1 imunologie   MeSH
• antigeny CD2 imunologie   MeSH
• antigeny CD45 imunologie   MeSH
• antigeny CD8 imunologie   MeSH
• buněčná diferenciace imunologie   MeSH
• buněčné dělení imunologie   MeSH
• CD antigeny imunologie   MeSH
• CD4-pozitivní T-lymfocyty imunologie   MeSH
• CD8-pozitivní T-lymfocyty imunologie   MeSH
• histokompatibilita - antigeny třídy II imunologie   MeSH
• imunofenotypizace metody   MeSH
• kultivované buňky MeSH
• modely u zvířat MeSH
• prasata MeSH
• receptory antigenů T-buněk gama-delta imunologie   MeSH
• slezina cytologie   imunologie   MeSH
• T-lymfocyty - podskupiny imunologie   MeSH
• thymus cytologie   embryologie   imunologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antigeny CD1 MeSH
• antigeny CD2 MeSH
• antigeny CD45 MeSH
• antigeny CD8 MeSH
• CD antigeny MeSH
• histokompatibilita - antigeny třídy II MeSH
• receptory antigenů T-buněk gama-delta MeSH