BACKGROUND: The aim of the study was to evaluate the usability of different fixative fluids in the detection of mast cells in ovaries and uteri of female dogs and cats. MATERIALS AND METHODS: Samples were fixed in 4% formaldehyde, Carnoy's fluid, Mota's basic lead acetate and isotonic formaldehyde-acetic acid (IFAA). RESULTS: Mast cells (MCs) were detected by acidified toluidine blue staining and counted for various parts of the ovaries and uteri. In the ovaries of both species, the numbers of MCs were significantly (p < 0.05) higher in Carnoy than in formalin. No significant differences were found between Carnoy and Mota (tested only in cats). In the uterus, numbers of MCs were significantly (p < 0.05) higher in Carnoy, Mota and IFAA compared to formalin (canine endometrium, feline endometrium and feline myometrium), in Carnoy and Mota compared to formalin (canine myometrium) and in Mota compared to IFAA (feline myometrium). The majority of MCs were formalin-sensitive in the canine and feline uterus, in the canine ovary and in the feline cortex ovarii. In the feline medulla ovarii, the majority of MCs were formalin-resistant. No formalin-resistant MCs were detected in the feline tunica albuginea ovarii. CONCLUSIONS: Thus, using Mota's or Carnoy's fluid in the canine or feline female reproductive organs is recommended. This study improves methodology for all studies which clarify the role of MCs in the reproductive organs of the domestic and laboratory animals.

• Klíčová slova
• Carnoy, Mota, dog, formol, ovary, uterus,
• MeSH
• fixace tkání metody   MeSH
• fixativa * MeSH
• kočky MeSH
• mastocyty * MeSH
• ovarium cytologie   MeSH
• psi MeSH
• uterus cytologie   MeSH
• zvířata MeSH
• Check Tag
• kočky MeSH
• psi MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• fixativa * MeSH

Keratocystic odontogenic tumour is relatively rare benign tumour. It is characterized by its fast aggressive growth and high risk of recurrence. Treatment is always surgical: conservative (enucleation, marsupialization) or aggressive (enucleation followed by application of Carnoy's solution, cryotherapy; peripheral ostectomy or en block resection of the jaw). Authors analysed retrospectively 22 patients who fulfilled inclusion criteria, i.e. had odontogenic keratocystic tumour of mandible, wherein antero-posterior dimension was at least 30 mm, and the tumour penetrated into the surrounding soft tissues. All patients underwent tumour enucleation, in 11 patients Carnoy's solution was given into the bone cavity after enucleation. The recurrence rate in the evaluation at least 36 months after surgery was both patient groups the same: 45.4%.

• Klíčová slova
• Carnoy’s solution, Enucleation, Keratocystic odontogenic tumour, Recurrence,
• MeSH
• chloroform terapeutické užití   MeSH
• dítě MeSH
• dospělí MeSH
• ethanol terapeutické užití   MeSH
• fixativa * MeSH
• kyselina octová terapeutické užití   MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• mladý dospělý MeSH
• nádory mandibuly patologie   terapie   MeSH
• odontogenní nádory patologie   terapie   MeSH
• retrospektivní studie MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• Check Tag
• dítě MeSH
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• Carnoy's solution MeSH Prohlížeč
• chloroform MeSH
• ethanol MeSH
• fixativa * MeSH
• kyselina octová MeSH

The effects of Zenker-formol, Carnoy, Orth and Bouin's fixatives on the development of acrosomal system during spermiogenesis in buffalo, goat and ram were compared. Using Zenker-formol, Orth and Bouin's fixatives it was found in the Golgi and cap phases that the acrosome is derived from the inner and outer zones of acrosomal system. With Carnoy's fixative it was seen that the weakly stained granules are surrounded by a vacuolar space. In the acrosome and maturation phases the acrosome and head cap stain uniformly in the tissue fixed in Zenker-formol, Orth and Bouin's fixatives. In Carnoy fixed tissue the PAS-positive material in the spermatids is weakly stained.

• MeSH
• acetáty farmakologie   MeSH
• akrozom účinky léků   MeSH
• buvoli fyziologie   MeSH
• chlorid rtuťnatý * MeSH
• chloroform farmakologie   MeSH
• chromany farmakologie   MeSH
• druhová specificita MeSH
• dvojchroman draselný farmakologie   MeSH
• ethanol farmakologie   MeSH
• fixativa farmakologie   MeSH
• fixní kombinace léků farmakologie   MeSH
• formaldehyd farmakologie   MeSH
• kozy fyziologie   MeSH
• kyselina octová * MeSH
• ovce fyziologie   MeSH
• pikráty farmakologie   MeSH
• rtuť farmakologie   MeSH
• sírany farmakologie   MeSH
• spermatogeneze * MeSH
• spermie účinky léků   MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH
• Názvy látek
• acetáty MeSH
• Bouin's solution MeSH Prohlížeč
• Carnoy's solution MeSH Prohlížeč
• chlorid rtuťnatý * MeSH
• chloroform MeSH
• chromany MeSH
• dvojchroman draselný MeSH
• ethanol MeSH
• fixativa MeSH
• fixní kombinace léků MeSH
• formaldehyd MeSH
• kyselina octová * MeSH
• Orth solution MeSH Prohlížeč
• pikráty MeSH
• rtuť MeSH
• sírany MeSH
• Zenker's solution MeSH Prohlížeč

We evaluated which of the fixatives and stains most frequently used for observation of insect tissues were the most appropriate for histopathological visualization of entomopathogenic fungal infections with Leptolegnia chapmanii in larvae of Aedes aegypti. The best contrast between the host tissues and the fungal structures was obtained when using a combination of Camoy fixative with Grocott staining contrasted with light green. Masson trichromic stain combined with 10% formaldehyde-phosphate buffer also provided satisfactory results--a good contrast and clearly distinguishable host tissues and fungal structures.

• MeSH
• Aedes růst a vývoj   mikrobiologie   MeSH
• chloroform MeSH
• ethanol MeSH
• fixativa MeSH
• histocytologické preparační techniky metody   MeSH
• kyselina octová MeSH
• larva mikrobiologie   MeSH
• oomycety patogenita   fyziologie   ultrastruktura   MeSH
• spory hub ultrastruktura   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• hodnotící studie MeSH
• Názvy látek
• Carnoy's solution MeSH Prohlížeč
• chloroform MeSH
• ethanol MeSH
• fixativa MeSH
• kyselina octová MeSH