This review focuses on analytical techniques used for separation and characterization of dendrimers and their derivatives. These macromolecules have been attractive material for a development of new drug carriers and imaging agents. They are also interesting for many biological and industrial applications. The review mentions a few of them.

• MeSH
• chemické techniky analytické MeSH
• dendrimery chemická syntéza   chemie   toxicita   MeSH
• kontrastní látky farmakologie   MeSH
• nosiče léků farmakologie   MeSH
• polyaminy chemie   MeSH
• polypropyleny chemie   MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH
• Názvy látek
• dendrimery MeSH
• kontrastní látky MeSH
• nosiče léků MeSH
• PAMAM Starburst MeSH Prohlížeč
• poly(propyleneimine) MeSH Prohlížeč
• polyaminy MeSH
• polypropyleny MeSH

This paper presents a synthesis of a novel nanoparticle label with selective biorecognition properties based on a biotinylated silver-dendrimer nanocomposite (AgDNC). Two types of labels, a biotin-AgDNC (bio-AgDNC) and a biotinylated AgDNC with a poly(ethylene)glycol spacer (bio-PEG-AgDNC), were synthesized from a generation 7 (G7) hydroxyl-terminated ethylenediamine-core-type (2-carbon core) PAMAM dendrimer (DDM) by an N,N'-dicyclohexylcarbodiimide (DDC) biotin coupling and a NaBH(4) silver reduction method. Synthesized conjugates were characterized by several analytical methods, such as UV-vis, FTIR, AFM, TEM, ELISA, HABA assay and SPR. The results show that stable biotinylated nanocomposites can be formed either with internalized silver nanoparticles (AgNPs) in a DMM polymer backbone ('type I') or as externally protected ('type E'), depending on the molar ratio of the silver/DMM conjugate and type of conjugate. Furthermore, the selective biorecognition function of the biotin is not affected by the AgNPs' synthesis step, which allows a potential application of silver nanocomposite conjugates as biospecific labels in various bioanalytical assays, or potentially as fluorescence cell biomarkers. An exploitation of the presented label in the development of electrochemical immunosensors is anticipated.

• MeSH
• avidin metabolismus   MeSH
• barvení a značení metody   MeSH
• biotin chemie   metabolismus   MeSH
• dendrimery MeSH
• ELISA MeSH
• kinetika MeSH
• mikroskopie atomárních sil MeSH
• molekulární modely MeSH
• nanokompozity chemie   MeSH
• polyaminy chemická syntéza   chemie   MeSH
• povrchová plasmonová rezonance MeSH
• spektrofotometrie ultrafialová MeSH
• spektroskopie infračervená s Fourierovou transformací MeSH
• stříbro chemie   MeSH
• transmisní elektronová mikroskopie MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• avidin MeSH
• biotin MeSH
• dendrimery MeSH
• PAMAM Starburst MeSH Prohlížeč
• polyaminy MeSH
• stříbro MeSH

Nanomaterials are in analytical science used for a broad range of purposes, covering the area of sample pretreatment as well as separation, detection, and identification of target molecules. This part of the review covers capillary electrophoresis (CE) of nanomaterials and focuses on the application of CE as a method for characterization used during nanomaterial synthesis and modification as well as the monitoring of their properties and interactions with other molecules. The heterogeneity of the nanomaterial family is extremely large. Depending on different definitions of the term Nanomaterial/Nanoparticle, the group may cover metal and polymeric nanoparticles, carbon nanomaterials, liposomes and even dendrimers. Moreover, these nanomaterials are usually subjected to some kind of surface modification or functionalization, which broadens the diversity even more. Not only for purposes of verification of nanomaterial synthesis and batch-to-batch quality check, but also for determination the polydispersity and for functionality characterization on the nanoparticle surface, has CE offered very beneficial capabilities. Finally, the monitoring of interactions between nanomaterials and other (bio)molecules is easily performed by some kind of capillary electromigration technique.

• Klíčová slova
• Capillary electrophoresis, Carbon nanotubes, Liposomes, Nanoparticles, Quantum dots,
• MeSH
• dendrimery chemie   MeSH
• elektroforéza kapilární metody   MeSH
• kovové nanočástice analýza   chemie   MeSH
• liposomy chemie   MeSH
• nanostruktury analýza   chemie   MeSH
• polymery chemie   MeSH
• poréznost MeSH
• povrchové vlastnosti MeSH
• uhlík chemie   MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH
• Názvy látek
• dendrimery MeSH
• liposomy MeSH
• polymery MeSH
• uhlík MeSH