The authors made a ten-year analysis of the incidence of these complications. They describe a total of 16 cases of arthralgia at the onset of diarrhoea and nine cases of reactive arthritis. The paper deals with some clinical and laboratory aspects of the disease.

• MeSH
• bakteriální infekce * diagnóza   terapie   MeSH
• dospělí MeSH
• lidé MeSH
• průjem mikrobiologie   MeSH
• reaktivní artritida diagnóza   etiologie   terapie   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH

• MeSH
• herpetické infekce komplikace   MeSH
• lidé MeSH
• revmatoidní artritida etiologie   mikrobiologie   MeSH
• virus Epsteinův-Barrové MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH

The most adequate means of diagnosing infections with caprine arthritis encephalitis (CAE) and Maedi-Visna (MV) viruses is the demonstration of antibodies to these viruses in milk or in blood serum. Various techniques and a range of different antigen preparations can be used for this purpose. We have evaluated two different whole virus antigen preparations derived from lamb synovial cells infected with CAE and MV viruses and recombinant antigen containing the capsid protein expressed in E. coli in ELISA test. The suitability of different detergents for solubilization of whole virus particles is shown in Tab. I. The detergents used were ether (50%, 10 min, 37 degrees C), octyl-beta-D glucopyranoside - OGP (2%, 2 h, 37 degrees C), and sodium dodecylsulphate SDS (0.125%, 10 min, 37 degrees C). Antigens prepared with SDS gave the best results and were then used in the following antigen comparative study. All antigens (whole virus and recombinant core protein) were used for the coating of ELISA plates and were evaluated on a panel of 130 sera. In general, whole virus antigens prepared from CAE- and MV-viruses gave identical results. The specificity achieved with recombinant antigen was superior but the sensitivity was lower compared with whole virus antigen. Immunoblotting served as the gold standard for discordant results. The other aim of our study was to compare antibody detection in blood serum and milk. Using whole virus antigen, antibodies could be detected with higher specificity and sensitivity in milk than in blood serum (Tab. II).(ABSTRACT TRUNCATED AT 250 WORDS)

• MeSH
• antigeny virové * MeSH
• ELISA * MeSH
• kozy MeSH
• lentivirové infekce diagnóza   veterinární   MeSH
• mléko mikrobiologie   MeSH
• nemoci koz diagnóza   MeSH
• protilátky virové analýza   MeSH
• virus artritidy a encefalitidy koz imunologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• srovnávací studie MeSH
• Názvy látek
• antigeny virové * MeSH
• protilátky virové MeSH

• MeSH
• artritida etiologie   MeSH
• dítě MeSH
• dospělí MeSH
• infekce komplikace   MeSH
• lidé MeSH
• věkové faktory MeSH
• Check Tag
• dítě MeSH
• dospělí MeSH
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

• MeSH
• dítě MeSH
• fokální infekce * MeSH
• juvenilní artritida * MeSH
• lidé MeSH
• předškolní dítě MeSH
• revmatická horečka * MeSH
• tonzilitida * MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• předškolní dítě MeSH
• Publikační typ
• časopisecké články MeSH

OBJECTIVES: Some adults with rheumatic and musculoskeletal diseases (RMDs) are at increased risk of COVID-19-related death. Excluding post-COVID-19 multisystem inflammatory syndrome of children, children and young people (CYP) are overall less prone to severe COVID-19 and most experience a mild or asymptomatic course. However, it is unknown if CYP with RMDs are more likely to have more severe COVID-19. This analysis aims to describe outcomes among CYP with underlying RMDs with COVID-19. METHODS: Using the European Alliance of Associations for Rheumatology COVID-19 Registry, the Childhood Arthritis and Rheumatology Research Alliance (CARRA) Registry, and the CARRA-sponsored COVID-19 Global Paediatric Rheumatology Database, we obtained data on CYP with RMDs who reported SARS-CoV-2 infection (presumptive or confirmed). Patient characteristics and illness severity were described, and factors associated with COVID-19 hospitalisation were investigated. RESULTS: 607 CYP with RMDs <19 years old from 25 different countries with SARS-CoV-2 infection were included, the majority with juvenile idiopathic arthritis (JIA; n=378; 62%). Forty-three (7%) patients were hospitalised; three of these patients died. Compared with JIA, diagnosis of systemic lupus erythematosus, mixed connective tissue disease, vasculitis, or other RMD (OR 4.3; 95% CI 1.7 to 11) or autoinflammatory syndrome (OR 3.0; 95% CI 1.1 to 8.6) was associated with hospitalisation, as was obesity (OR 4.0; 95% CI 1.3 to 12). CONCLUSIONS: This is the most significant investigation to date of COVID-19 in CYP with RMDs. It is important to note that the majority of CYP were not hospitalised, although those with severe systemic RMDs and obesity were more likely to be hospitalised.

• Klíčová slova
• Arthritis, Juvenile, Covid-19, Infections,
• MeSH
• COVID-19 * komplikace   epidemiologie   MeSH
• dítě MeSH
• juvenilní artritida * komplikace   epidemiologie   MeSH
• lidé MeSH
• mladiství MeSH
• mladý dospělý MeSH
• muskuloskeletální nemoci * epidemiologie   MeSH
• obezita komplikace   MeSH
• revmatické nemoci * komplikace   epidemiologie   MeSH
• SARS-CoV-2 MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• mladiství MeSH
• mladý dospělý MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Research Support, N.I.H., Extramural MeSH

BACKGROUND: To derive a list of opportunistic infections (OI) through the analysis of the juvenile idiopathic arthritis (JIA) patients in the Pharmachild registry by an independent Safety Adjudication Committee (SAC). METHODS: The SAC (3 pediatric rheumatologists and 2 pediatric infectious disease specialists) elaborated and approved by consensus a provisional list of OI for use in JIA. Through a 5 step-procedure, all the severe and serious infections, classified as per MedDRA dictionary and retrieved in the Pharmachild registry, were evaluated by the SAC by answering six questions and adjudicated with the agreement of 3/5 specialists. A final evidence-based list of OI resulted by matching the adjudicated infections with the provisional list of OI. RESULTS: A total of 772 infectious events in 572 eligible patients, of which 335 serious/severe/very severe non-OI and 437 OI (any intensity/severity), according to the provisional list, were retrieved. Six hundred eighty-two of 772 (88.3%) were adjudicated as infections, of them 603/682 (88.4%) as common and 119/682 (17.4%) as OI by the SAC. Matching these 119 opportunistic events with the provisional list, 106 were confirmed by the SAC as OI, and among them infections by herpes viruses were the most frequent (68%), followed by tuberculosis (27.4%). The remaining events were divided in the groups of non-OI and possible/patient and/or pathogen-related OI. CONCLUSIONS: We found a significant number of OI in JIA patients on immunosuppressive therapy. The proposed list of OI, created by consensus and validated in the Pharmachild cohort, could facilitate comparison among future pharmacovigilance studies. TRIAL REGISTRATION: Clinicaltrials.gov NCT01399281; ENCePP seal: awarded on 25 November 2011.

• Klíčová slova
• Biologics, Immunosuppressive therapy, Infections, Juvenile idiopathic arthritis, Opportunistic,
• MeSH
• antirevmatika terapeutické užití   MeSH
• dítě MeSH
• farmakovigilance MeSH
• herpes zoster diagnóza   etiologie   MeSH
• hostitel s imunodeficiencí * MeSH
• juvenilní artritida komplikace   farmakoterapie   patologie   MeSH
• kohortové studie MeSH
• lidé MeSH
• oportunní infekce diagnóza   etiologie   MeSH
• předškolní dítě MeSH
• registrace statistika a číselné údaje   MeSH
• stupeň závažnosti nemoci MeSH
• tuberkulóza diagnóza   etiologie   MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• mužské pohlaví MeSH
• předškolní dítě MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• klinické zkoušky MeSH
• multicentrická studie MeSH
• pozorovací studie MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antirevmatika MeSH

The balance between the tumor-necrosis factor α (TNFα) and type-I interferon (T1IFN) pathways is crucial for proper immune function. Dysregulation of either pathway can contribute to autoimmune diseases development. Even though TNFα blockade has shown promising results in various autoimmune diseases, the effect on the balance between TNFα and T1IFN is elusive. We used targeted anti-TNFα therapies in juvenile idiopathic arthritis (JIA) as an experimental approach to study the cross-regulation between TNFα and type-I IFN. We found that TNFα-rich environment affected viral defense through the attenuation of T1IFN responses and affected the phenotype and distribution of myeloid dendritic cells, which are engaged in early viral infections. Anti-TNFα therapy normalized the observed deviations in JIA patients. We hypothesize that the inadequate immune response caused by a high TNFα environment could be projected to more frequent or lengthy viral infections and possibly play a role in the process of JIA disease development.

• Klíčová slova
• Balance, Interferon, Juvenile idiopathic arthritis, TNFα, Viral infections,
• MeSH
• dendritické buňky MeSH
• fenotyp MeSH
• interferon typ I * MeSH
• juvenilní artritida * farmakoterapie   MeSH
• lidé MeSH
• nekróza MeSH
• TNF-alfa MeSH
• virové nemoci * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• interferon typ I * MeSH
• TNF-alfa MeSH