The European LeukemiaNet MDS (EUMDS) registry is collecting data of myelodysplastic syndrome (MDS) patients belonging to the IPSS low or intermediate-1 category, newly diagnosed by local cytologists. The diagnosis of MDS can be challenging, and some data report inter-observer variability with regard to the assessment of the MDS subtype. In order to ensure that correct diagnoses were made by the participating centres, blood and bone marrow slides of 10% of the first 1000 patients were reviewed by an 11-person panel of cytomorphologists. All slides were rated by at least 3 panel members (median 8 panel members; range 3-9). Marrow slides from 98 out of 105 patients were of good quality and therefore could be rated properly according to the WHO 2001 classification, including assessment of dysplastic lineages. The agreement between the reviewers whether the diagnosis was MDS or non-MDS was strong with an intra-class correlation coefficient (ICC) of 0.85. Six cases were detected not to fit the entry criteria of the registry, because they were diagnosed uniformly as CMML or AML by the panel members. The agreement by WHO 2001 classification was strong as well (ICC = 0.83). The concordance of the assessment of dysplastic lineages was substantial for megakaryopoiesis and myelopoiesis and moderate for erythropoiesis. Our data show that in general, the inter-observer agreement was high and a very low percentage of misdiagnosed cases had been entered into the EUMDS registry. Further studies including histomorphology are warranted.

• Klíčová slova
• Cytomorphology review, Diagnostics, Inter-observer variability, Myelodysplastic syndromes (MDS),
• MeSH
• cytodiagnostika metody   normy   MeSH
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladý dospělý MeSH
• myelodysplastické syndromy krev   diagnóza   MeSH
• odchylka pozorovatele * MeSH
• registrace statistika a číselné údaje   MeSH
• reprodukovatelnost výsledků MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• senzitivita a specificita MeSH
• vyšetřování kostní dřeně metody   normy   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH

The hypophyseal ACTH (basophil type 3 or B3), cells of R. tigerina display seasonal changes in cytomorphology (nuclear, cytoplasm and cell area), staining intensity and cytoplasmic granulation. The cytoplasmic granules are fine and stain blackish purple with McConaill's lead haematoxylin, with Herlant's AB and the PAS and OG techniques they stain brownish red. The tinctorial response of their cytoplasm is PAS- and OG-positive and AB-negative. The B3 cells are low columnar or cuboidal in form and are smaller than the gonadotrophs (B2 cells). Towards the rostral border of the pars distalis they are larger and are associated with blood vessels, whereas in the caudal region they are smaller. Seasonal changes in the granulation and degranulation of the ACTH cells are also more prominent in the rostral region of the pars distalis than in the caudal region. During April, May and June a marked increase occurs in ACTH cell size and the number of cytoplasmic secretory granules, concurrently with events like vitellogenesis, lipid depletion from the fat bodies and breeding activity. During the post-breeding regression phase (and especially from August to November), the B3 cells decrease markedly in size and become devoid of secretory granules; their recovery begins slowly from December onwards. The findings suggest that the secretory activity of the ACTH cells in the frog undergoes seasonal changes which possibly influence vitellogenesis, lipolysis of the fat bodies and breeding activity.

• MeSH
• adrenokortikotropní hormon metabolismus   MeSH
• hypofýza cytologie   MeSH
• Ranidae anatomie a histologie   metabolismus   MeSH
• roční období * MeSH
• rozmnožování fyziologie   MeSH
• zvířata MeSH
• Check Tag
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH
• Názvy látek
• adrenokortikotropní hormon MeSH

Two zooid forming strains and four non-zooid strains of the green chlorococcal alga Scenedesmus obliquus were compared in terms of growth, morphological and physiological characteristics. Large differences were observed among the strains grown under various growth conditions (light and temperature). The assumption that the zooid forming strains may be similar was not confirmed. Since they considerably differed in daughter cells morphology, photosynthesis, growth rate in batch culture or commitment to cellular division. Molecular-genetic comparison of 18S RNA/DNA might distinguish zooid forming strains from non-zooid ones.

• MeSH
• buněčný cyklus fyziologie   MeSH
• chlorofyl metabolismus   MeSH
• fotosyntéza fyziologie   MeSH
• koncentrace vodíkových iontů MeSH
• pohyb buněk fyziologie   MeSH
• Scenedesmus * klasifikace   cytologie   fyziologie   MeSH
• spory fyziologie   MeSH
• světlo MeSH
• vysoká teplota MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• chlorofyl MeSH

Circulating tumor cells (CTC) may reach multiple organ sites. However, CTC seeding and growth in distant organs is not random. Each metastatic site may contain a specific subpopulation of the original metastatic tumor capable of growing at that site. The fluorescent orthotopic prostate cancer model (PC-3-GFP) model was used for immunomagnetic capture of CTC. The captured CTC were efficiently cultivated in vitro. PC-3-GFP cells were also isolated from various metastatic sites, grown in vitro and examined under fluorescence microscopy. The differential morphology was compared of primary tumor cells, CTC and disseminated (DTC) from multiple metastatic sites, from nude mice with orthotopic PC-3-GFP. The cultured captured CTC and DTC from various organs have distinctive morphologies. Distinct cancer cell morphologies were observed at different metastatic sites as well as among CTC. The distinct morphologies were maintained during in vitro culture. The results demonstrate extensive tumor heterogeneity that could account for the widely different behavior of cancer cells in a single tumor. Further hetereogeneity testing would be a big promise for personalizing the cancer treatment in the future.

• MeSH
• fluorescenční mikroskopie MeSH
• imunomagnetická separace MeSH
• lidé MeSH
• myši nahé MeSH
• myši MeSH
• nádorové buněčné linie MeSH
• nádorové cirkulující buňky metabolismus   patologie   MeSH
• nádory prostaty metabolismus   patologie   MeSH
• orgánová specificita MeSH
• transplantace nádorů MeSH
• tvar buňky MeSH
• zelené fluorescenční proteiny biosyntéza   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• zelené fluorescenční proteiny MeSH

• MeSH
• cytodiagnostika MeSH
• cytologické techniky MeSH
• karcinom diagnóza   patologie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• nádory endometria diagnóza   patologie   MeSH
• prognóza MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH

As cancer care is transitioning to personalized therapies with necessary complementary or companion biomarkers there is significant interest in determining to what extent non-invasive liquid biopsies reflect the gold standard solid biopsy. We have established an approach for measuring patient-specific circulating and solid cell concordance by introducing tumor touch preparations to the High-Definition Single Cell Analysis workflow for high-resolution cytomorphometric characterization of metastatic colorectal cancer (mCRC). Subgroups of cells based on size, shape and protein expression were identified in both liquid and solid biopsies, which overall displayed high inter- and intra- patient pleomorphism at the single-cell level of analysis. Concordance of liquid and solid biopsies was patient-dependent and between 0.1-0.9. Morphometric variables displayed particularly high correlation, suggesting that circulating cells do not represent distinct subpopulations from the solid tumor. This was further substantiated by significant decrease in concentration of circulating cells after mCRC resection. Combined with the association of circulating cells with tumor burden and necrosis of hepatic lesions, our overall findings demonstrate that liquid biopsy cells can be informative biomarkers in the mCRC setting. Patient-specific level of concordance can readily be measured to establish the utility of circulating cells as biomarkers and define biosignatures for liquid biopsy assays.

• Klíčová slova
• circulating tumor cells, cytomorphology, liquid biopsy, metastatic colorectal cancer, single cell analysis,
• Publikační typ
• časopisecké články MeSH

BACKGROUND: A low-risk thyroid tumour, non-invasive follicular thyroid neoplasm with papillary-like nuclear features (NIFTP) was introduced in 2016. NIFTP criteria require a thorough histological examination to rule out capsular and lymphovascular invasion, which denies the possibility of preoperative cytological diagnosis. Nevertheless, since the adoption of the new entity, the cytology of NIFTP has been a subject of interest. OBJECTIVES: The present systematic review and meta-analysis investigate the cytological diagnosis of NIFTP. METHOD: An online PubMed literature search was conducted between March 1, 2020, and June 30, 2020, for all original articles considering the cytology of histologically proven NIFTP. The studies including data on fine needle aspiration specimens classified by The Bethesda System for Reporting Thyroid Cytology (TBSRTC) categories, risk of malignancy (ROMs) in the TBSRTC categories, and cytomorphological features of NIFTP were included in the meta-analysis. Non-English studies and case reports were excluded. The data were tabulated and statistical analysis was performed with Open Meta-Analyst program. RESULTS: Fifty-eight studies with a total of 2,553 NIFTP cases were included in the study. The pooled prevalence of NIFTP cases was calculated among 25,892 surgically resected cases from 20 studies and the results show that NIFTP consisted 4.4% (95% confidence interval [CI]: 3.5-5.4%) of all cases. Most of the NIFTP cases (79.0%) belonged to the intermediate categories of TBSRTC. The pooled distribution of NIFTP cases in each TBSRTC category was 1.3% (95% CI: 0.8-1.7%) in non-diagnostic (ND), 8.9% (95% CI: 6.9-10.8%) in benign, 29.2% (95% CI: 25.0-33.4%) in atypia of undetermined significance or follicular lesion of undetermined significance (AUS/FLUS), 24.2% (95% CI: 19.6-28.9%) in follicular neoplasm (FN), 19.5% (95% CI: 16.1-22.9%) in suspicious for malignancy (SM), and 6.9% (95% CI: 5.2-8.7%) in malignant. Compared to pre-NIFTP era, the pooled risk differences of ROM were reduced by 2.4% in ND, 2.7% in benign, 8.2% in AUS/FLUS, 8.2% in FN, 7.3% in SM, and 1.1% in the malignant category. The cytomorphological features of NIFTP were similar to follicular variant of papillary thyroid carcinoma (FVPTC) but lesser to papillary thyroid carcinoma (PTC). CONCLUSIONS: Based on our results, NIFTP remains a histological diagnosis. Although cytomorphological features cannot be used in differentiating NIFTP from FVPTC, they may guide in separating NIFTP from PTC. Features such as papillae, microfollicles, giant cells, psammoma bodies, and the amount of papillary-like nuclear features should be taken into account when suspicious of NIFTP. NIFTP should not have papillae or psammoma bodies, and giant cells were rarely observed.

• Klíčová slova
• Cytomorphology, Meta-analysis, Non-invasive follicular thyroid neoplasm with papillary-like nuclear features, The Bethesda System for Reporting Thyroid Cytology, Thyroid gland,
• MeSH
• cytodiagnostika metody   MeSH
• folikulární adenokarcinom * diagnóza   patologie   MeSH
• lidé MeSH
• nádory štítné žlázy * patologie   MeSH
• papilární karcinom štítné žlázy diagnóza   patologie   MeSH
• tenkojehlová biopsie MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• metaanalýza MeSH
• systematický přehled MeSH

• Klíčová slova
• MEDICINE, LEGAL *, SEX CHARACTERISTICS *,
• MeSH
• lidé MeSH
• pohlavní dimorfismus * MeSH
• soudní lékařství * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

• Klíčová slova
• LUPUS ERYTHEMATOSUS *,
• MeSH
• systémový lupus erythematodes * MeSH
• Publikační typ
• časopisecké články MeSH

Defoaming of the medium by a rotating turbine during yeast cultivation, in contrast to defoaming by oil, results in fading away of cells of higher age categories and in an increase of dead cells proportion in the population. The negative effect of the mechanical defoaming device did not manifest itself in the amount of biomass obtained but in the cell numbers observed.

• MeSH
• kultivační média MeSH
• mechanický stres * MeSH
• odpěňovadla farmakologie   MeSH
• povrchově aktivní látky farmakologie   MeSH
• rotace MeSH
• Saccharomyces cerevisiae cytologie   účinky léků   růst a vývoj   MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH
• Názvy látek
• kultivační média MeSH
• odpěňovadla MeSH
• povrchově aktivní látky MeSH