• Klíčová slova
• TEETH EXTRACTION *,
• MeSH
• extrakce zubů * MeSH
• lidé MeSH
• seriová extrakce * MeSH
• zubní oblouk * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

Nowadays, regenerative and reparative medicine has grown in popularity. Dental stem cells are easily accessible source of adult stem cells. They can be harvested by a tooth extraction or spontaneous deciduous tooth exfoliation. They have to be isolated, expanded and stored until time they would be needed for individual stem cell therapy. Cryopreservation is both a short-term and long-term storage of tissues or cells at sub-zero temperatures. There are several methods of cryopreservation requiring different technologies. The objective of this review is to compare them and highlight their advantages and disadvantages.

• Klíčová slova
• cryopreservation, dental stem cells, freezing protocol,
• MeSH
• dospělé kmenové buňky cytologie   MeSH
• extrakce zubů MeSH
• kryoprezervace metody   MeSH
• lidé MeSH
• regenerativní lékařství MeSH
• transplantace kmenových buněk * MeSH
• vitrifikace * MeSH
• vypadávání zubů MeSH
• zubní dřeň cytologie   MeSH
• zuby mléčné cytologie   MeSH
• zuby cytologie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH

Dental health has historically received little attention in veterinary medicine, but is becoming more common. This study aimed to report the prevalence of dental extractions in dogs in Seoul, Republic of Korea, describe the current status of dental health and determine any preventive methods. In total, 166 dogs participated in the study, presented to the veterinary hospital with an oral disorder or for a routine check-up were included in the study. Teeth were extracted from 130 dogs (78.32%). A single tooth was extracted from 18 dogs (13.85%), whereas multiple teeth (2-29 teeth) were extracted from 112 dogs (86.15%). Ten teeth were extracted in 31 dogs (27.67%). In descending order, the most extracted teeth were PM2, PM3, PM4, and PM1. The age at the first dental check-up, the average interval between dental check-ups, and the average interval between the previous two dental check-ups in the extraction group were significantly greater than those in the non-extraction group. In contrast, the number of dental check-ups was significantly lower. In conclusion, this study suggests regular dental check-ups to prevent dental extractions. The data provide useful information for veterinary dental health management and the prevention of tooth extractions.

• Klíčová slova
• age, dental extraction, dogs, prevalence, preventive,
• Publikační typ
• časopisecké články MeSH

The authors assessed the diagnostic reasons for extraction of 1170 teeth in 565 patients treated in a health community dental centre. They recorded data on the patient's sex and age, on the extracted tooth and reason for extraction. They found that under the age of 30 years permanent teeth were extracter mostly on account of extensive damage by caries and its sequelae. On account of destruction of the periodontium permanent teeth were extracted in patients older than 31 years. At the age of 31-40 years on account of extensive caries and its complications significantly more teeth were extracted in women than in men, at the age above 71 years significantly more teeth were extracted on account of this diagnosis in men, as compared with women. On account of caries and its sequelae 75.7% upper premolars, 69.7% upper molars and 69.9% lower molars were extracted. On account of advanced destruction of the periodontium in the investigated group 80.5% lower incisors were extracted. In other groups of teeth this diagnosis was less frequent.

• MeSH
• dospělí MeSH
• extrakce zubů statistika a číselné údaje   MeSH
• komunitní zdravotní střediska MeSH
• lidé středního věku MeSH
• lidé MeSH
• senioři MeSH
• ztráta zubů etiologie   MeSH
• zubní kaz komplikace   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• Geografické názvy
• Československo epidemiologie   MeSH

Dental calculus is becoming a crucial material in the study of past populations with increasing interest in its proteomic and genomic content. Here, we suggest further development of a protocol for analysis of ancient proteins and a combined approach for subsequent ancient DNA extraction. We tested the protocol on recent teeth, and the optimized protocol was applied to ancient tooth to limit the destruction of calculus as it is a precious and irreplaceable source of dietary, microbiological, and ecological information in the archeological context. Finally, the applicability of the protocol was demonstrated on samples of the ancient calculus.

• Klíčová slova
• ancient DNA, ancient proteins, bioarcheology, dental calculus, paleoproteomics,
• Publikační typ
• časopisecké články MeSH

INTRODUCTION: Risk factors for developing osteoradionecrosis (ORN) are well known, but less is known about factors influencing the interval between radiotherapy and the onset of ORN. Also, it is unknown whether there is any specific period post-radiotherapy with a reduced probability of ORN when irradiated teeth require extraction. PURPOSE: The primary aim of this study was to identify factors influencing the interval in developing ORN in the following subgroups of patients: (1) patients who spontaneously developed ORN, (2) surgical-intervention-related ORN with a particular focus on patients after mandibulectomy. The secondary aim was to attempt to identify a possible time for safer dental intervention after primary treatment. MATERIALS AND METHODS: The authors retrospectively analysed 1608 head and neck cancer (HNC) patients treated in a single centre. Time intervals were measured from the end of radiotherapy to the development of ORN and further analysed in the subgroups listed above. RESULTS: In all, 141 patients (8.8%) developed intra-oral ORN. Median time from radiotherapy to ORN development in the whole cohort was 9 months. Median interval for spontaneous ORN was 8 months, 6.5 months for intervention-related ORN, and 15 months for patients post-mandibulectomy. In patients who required dental extraction preradiotherapy, median interval of ORN onset was 5 months. CONCLUSION: In our study, a slightly higher proportion of patients with intervention developed ORN earlier in comparison with spontaneous ORN. The period from 12-18 months after radiotherapy was identified as having the highest probability of developing ORN in patients after mandibulectomy. A time for safer dental intervention after primary treatment was not identified.

• Klíčová slova
• Dental intervention, Head and neck cancer, Osteoradionecrosis, Radiotherapy, Time factors,
• MeSH
• dospělí MeSH
• extrakce zubů * MeSH
• lidé středního věku MeSH
• lidé MeSH
• mandibulotomie MeSH
• nádory hlavy a krku radioterapie   chirurgie   MeSH
• osteoradionekróza * etiologie   MeSH
• retrospektivní studie MeSH
• rizikové faktory MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH

• Klíčová slova
• ORTHODONTICS *, TEETH EXTRACTION *,
• MeSH
• extrakce zubů * MeSH
• lidé MeSH
• ortodoncie * MeSH
• ortopedické výkony * MeSH
• ortopedie * MeSH
• stomatologická péče * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

Many dental and periodontal diseases are largely a question of bacterial etiology. Dental caries develop due to an increase of strongly acidogenic and aciduric gram-positive bacteria while common forms of periodontal disease are linked to anaerobic gram-negative bacteria in subgingival plaque. Many plants and plant-derived antimicrobial components are used in folklore therapeutics for the treatment of periodontal disorders and for the purposes of oral hygiene. Some have been evaluated for possible use in modern medicine, while thousands of other potentially useful/plants have not been tested. In this study, we evaluated the feasibility of screening for antibacterials isolated from plants with activity against three representatives of oral streptococci. We developed and tested the following methodologies: (1) Extraction of antibacterial components from plants; (2) Assays for antibacterial activity; (3) Chromatographic methods for initial analysis of compounds of interest. The screening process for plant antimicrobials consisted of extraction of plant material and assay of antibacterial activity using a spotting test with the selected oral streptococci as indicator strains. In addition, we developed chromatographic procedures that allow characterization and optimization of initial isolation steps. Depending on the indicator microorganisms used, the screening assay can target additional pathogens including other streptococci (group A and B, and pneumococci) and periodontal pathogens such as Porphyromonas. Also, we noted that the activity of some extracts varied against different oral bacteria. Our conclusion, supported by extensive data, was that the screening for antimicrobials from plants is a feasible approach to the identification of natural compounds with antimicrobial properties against dental pathogens.

• MeSH
• antibakteriální látky izolace a purifikace   farmakologie   MeSH
• chromatografie na tenké vrstvě MeSH
• lidé MeSH
• mikrobiální testy citlivosti MeSH
• rostlinné extrakty izolace a purifikace   farmakologie   MeSH
• Streptococcus mutans účinky léků   MeSH
• Streptococcus sanguis účinky léků   MeSH
• Streptococcus sobrinus účinky léků   MeSH
• studie proveditelnosti MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antibakteriální látky MeSH
• rostlinné extrakty MeSH

• Klíčová slova
• TEETH *, TEETH EXTRACTION *,
• MeSH
• extrakce zubů * MeSH
• gangréna * MeSH
• lidé MeSH
• zubní dřeň * MeSH
• zuby * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

OBJECTIVE: Dental caries is one of the most frequent multifactorial diseases. Among the numerous factors influencing the risk of caries, genetics plays a substantial role, with heritability ranging from 40 to 60%. Gene variants affecting taste preference and glucose transport were recently associated with caries risk. The aim of this study was to analyze two common polymorphisms in the sweet taste receptor (TAS1R2) and glucose transporter (GLUT2) genes in children with dental caries and healthy controls in the Czech population. METHODS: A total of 637 unrelated Caucasian children, aged 11-13 years, were included in this case-control study. One hundred and fifty-five subjects were caries-free (with decayed/missing/filled teeth, DMFT = 0) and 482 children were caries-affected (DMFT ≥ 1). The TAS1R2 (Ile191Val, rs35874116) and GLUT2 (Thr110Ile, rs5400) genotypes were determined using the 5' nuclease TaqMan® assay for allelic discrimination. RESULTS: Compared with subjects with the common Thr allele, carriers of the Ile allele of GLUT2 had significantly more frequently dental caries (p < 0.05, OR = 1.639, 95% CI: 1.089-2.466). Similarly, children with the Val allele for the TAS1R2 Ile191Val polymorphism were more frequently affected by caries than children who carried the Ile allele (p < 0.05, OR = 1.413, 95% CI: 1.014-1.969). In contrast, no significant associations between GLUT2 and/or TAS1R2 polymorphisms and fillings were found, but allele frequencies of the TAS1R2 variant were marginally significantly different between children with DMFT = 0 and DMFT ≥1 (p = 0.053, OR = 1.339, 95% CI: 0.996-1.799). However, no significant interaction between both genes and risk of dental caries was found. CONCLUSIONS: In conclusion, GLUT2 and TASR1 polymorphisms may influence the risk of caries in the Czech population.

• MeSH
• dítě MeSH
• DMF Index MeSH
• extrakce zubů statistika a číselné údaje   MeSH
• frekvence genu genetika   MeSH
• genetická variace genetika   MeSH
• genotyp MeSH
• guanin MeSH
• indexy plaku MeSH
• isoleucin genetika   MeSH
• jednonukleotidový polymorfismus genetika   MeSH
• lidé MeSH
• mladiství MeSH
• náchylnost k zubnímu kazu genetika   MeSH
• parodontální index MeSH
• polymorfismus genetický genetika   MeSH
• přenašeč glukosy typ 2 genetika   MeSH
• receptory spřažené s G-proteiny genetika   MeSH
• studie případů a kontrol MeSH
• threonin genetika   MeSH
• thymin MeSH
• trvalá zubní náhrada statistika a číselné údaje   MeSH
• valin genetika   MeSH
• zubní kaz genetika   MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• mladiství MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• Geografické názvy
• Česká republika MeSH
• Názvy látek
• guanin MeSH
• isoleucin MeSH
• přenašeč glukosy typ 2 MeSH
• receptory spřažené s G-proteiny MeSH
• SLC2A2 protein, human MeSH Prohlížeč
• taste receptors, type 1 MeSH Prohlížeč
• threonin MeSH
• thymin MeSH
• valin MeSH