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The European Space Agency (ESA) is currently expanding its efforts in identifying requirements and promoting research towards optimizing radiation protection of astronauts. Space agencies use common limits for tissue (deterministic) effects on the International Space Station. However, the agencies have in place different career radiation exposure limits (for stochastic effects) for astronauts in low-Earth orbit missions. Moreover, no specific limits for interplanetary missions are issued. Harmonization of risk models and dose limits for exploratory-class missions are now operational priorities, in view of the short-term plans for international exploratory-class human missions. The purpose of this paper is to report on the activity of the ESA Topical Team on space radiation research, whose task was to identify the most pertinent research requirements for improved space radiation protection and to develop a European space radiation risk model, to contribute to the efforts to reach international consensus on dose limits for deep space. The Topical Team recommended ESA to promote the development of a space radiation risk model based on European-specific expertise in: transport codes, radiobiological modelling, risk assessment, and uncertainty analysis. The model should provide cancer and non-cancer radiation risks for crews implementing exploratory missions. ESA should then support the International Commission on Radiological Protection to harmonize international models and dose limits in deep space, and guarantee continuous support in Europe for accelerator-based research configured to improve the models and develop risk mitigation strategies.
- Klíčová slova
- Dose limits, Risk assessment, Space radiation,
- MeSH
- dávka záření MeSH
- hodnocení rizik metody MeSH
- incidence MeSH
- kosmické záření škodlivé účinky MeSH
- kosmický let MeSH
- kosmonauti MeSH
- lidé MeSH
- nádory vyvolané zářením epidemiologie MeSH
- radiační ochrana normy MeSH
- radiační poranění epidemiologie MeSH
- radiobiologie MeSH
- výzkumný projekt * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Evropa epidemiologie MeSH
- MeSH
- biologické jevy * MeSH
- biomedicínský výzkum metody MeSH
- lidé MeSH
- neurologie MeSH
- psychiatrie * MeSH
- psychologie * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- úvodníky MeSH
- Klíčová slova
- BEHAVIOR, ANIMAL *, CHLORPROMAZINE *, EXPERIMENTAL LAB STUDY *, MICE *, PERIODICITY *, PSYCHOPHARMACOLOGY *,
- MeSH
- chlorpromazin * MeSH
- chování zvířat * MeSH
- myši MeSH
- periodicita * MeSH
- psychofarmakologie * MeSH
- výzkum * MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- chlorpromazin * MeSH
The relationship between physiological and behavioral stress markers is documented in several rodent species. However, there is no information regarding the role of adrenocortical activity in behavior of the black rat (Rattus rattus). Therefore, we hypothesize that the adrenocortical activity of black rats varies between individuals and is related to some of the behaviors in a novel environment. To test this hypothesis, we (i) validated a method for quantifying glucocorticoid metabolites from feces (fGCMs) with an enzyme immunoassay (EIA); (ii) examined variation and diurnal rhythms of feces and GCM production; and (iii) examined the relationship between GCM levels and exploratory behavioral traits. We fulfilled the first aim (i) by successfully performing an ACTH challenge test to validate the use of a 5α-pregnane-3β,11β,21-triol-20-one EIA for measuring fGCMs. Second (ii) we detected considerable consistent interindividual variability in production of both feces and glucocorticoids. The peak production of feces occurred in the first hour of the dark cycle, the peak of fGCMs occurred approximately 3 h later. Lastly, (iii) there was no clear relationship between behavior in the hole board test and GCMs. Grooming, a typical behavioral stress marker, was negatively associated with stress reactivity, while head-dipping in the hole-board test (traditionally considered an exploratory behavior independent of stress) was not correlated with the GCMs. This study offers a first look at GCMs in the black rat, successfully validates a method for their measurement and opens possibilities for future research of the relationship between glucocorticoids and exploratory behavior in this species.
- Klíčová slova
- ACTH challenge test, coping styles, feces, hole-board test, ship rat,
- MeSH
- chování zvířat MeSH
- divoká zvířata MeSH
- feces chemie MeSH
- glukokortikoidy chemie metabolismus MeSH
- imunoenzymatické techniky MeSH
- krysa rodu Rattus fyziologie MeSH
- kůra nadledvin fyziologie MeSH
- pátrací chování fyziologie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus fyziologie MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- glukokortikoidy MeSH
In the present study, the effect of the medial septal (MS) lesions on exploratory activity in the open field and the spatial and object recognition memory has been investigated. This experiment compares three types of MS lesions: electrolytic lesions that destroy cells and fibers of passage, neurotoxic - ibotenic acid lesions that spare fibers of passage but predominantly affect the septal noncholinergic neurons, and immunotoxin - 192 IgG-saporin infusions that only eliminate cholinergic neurons. The main results are: the MS electrolytic lesioned rats were impaired in habituating to the environment in the repeated spatial environment, but rats with immuno- or neurotoxic lesions of the MS did not differ from control ones; the MS electrolytic and ibotenic acid lesioned rats showed an increase in their exploratory activity to the objects and were impaired in habituating to the objects in the repeated spatial environment; rats with immunolesions of the MS did not differ from control rats; electrolytic lesions of the MS disrupt spatial recognition memory; rats with immuno- or neurotoxic lesions of the MS were normal in detecting spatial novelty; all of the MS-lesioned and control rats clearly reacted to the object novelty by exploring the new object more than familiar ones. Results observed across lesion techniques indicate that: (i) the deficits after nonselective damage of MS are limited to a subset of cognitive processes dependent on the hippocampus, (ii) MS is substantial for spatial, but not for object recognition memory - the object recognition memory can be supported outside the septohippocampal system; (iii) the selective loss of septohippocampal cholinergic or noncholinergic projections does not disrupt the function of the hippocampus to a sufficient extent to impair spatial recognition memory; (iv) there is dissociation between the two major components (cholinergic and noncholinergic) of the septohippocampal pathway in exploratory behavior assessed in the open field - the memory exhibited by decrements in exploration of repeated object presentations is affected by either electrolytic or ibotenic lesions, but not saporin.
- MeSH
- bludiště - učení účinky léků fyziologie MeSH
- hipokampus účinky léků patologie fyziologie MeSH
- imunotoxiny toxicita MeSH
- krysa rodu Rattus MeSH
- neurotoxiny toxicita MeSH
- pátrací chování účinky léků fyziologie MeSH
- potkani Wistar MeSH
- rozpoznávání (psychologie) účinky léků fyziologie MeSH
- septální jádra účinky léků patologie fyziologie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- imunotoxiny MeSH
- neurotoxiny MeSH
The genus Acomys is of growing importance to many research fields. Previous research has shown that individuals differ when exploring new environments and that these behavioural strategies are consistent in time. In this study, we subjected 60 commensal Acomys cahirinus (37 males, 23 females) to a series of seven tests (free exploration, forced exploration under bright illumination, forced exploration under low illumination, hole board test, vertical activity test, elevated plus maze, and voluntary wheel running) to acquire independent behavioural traits and investigate whether and how personality develops in spiny mice. The full series of experiments was performed twice during ontogeny: once in the sub-adult stage (tested at 62-72 days of age) and once in the adult stage (102-112 days of age). We found that behaviour of the animals was repeatable both within (range of R values from 0.155 to 0.726) and across the two life-stages (0.238 to 0.563). While the structure of behaviour in adults was rather clear, it had not been fully crystalized in sub-adults, suggesting personality changes during maturation, even though some individual traits might be repeatable across ontogeny. Notably, the most consistent behavioural traits across the different tests were jumping and rearing, which are not commonly reported.
- Klíčová slova
- Development, Elevated plus maze, Hole board test, Ontogeny, Open field test, Personality, Voluntary wheel running,
- MeSH
- kognice MeSH
- Murinae MeSH
- osobnost MeSH
- pátrací chování * MeSH
- pohybová aktivita * MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
The visual evaluation of data derived from screening and optimization experiments in the development of new analytical methods poses a considerable time investment and introduces the risk of subjectivity. This study presents a novel approach to processing such data, based on factor analysis of mixed data and hierarchical clustering - multivariate techniques implemented in the R programming language. The methodology is demonstrated in the early-stage screening and optimization of the chromatographic separation of 15 structurally diverse drugs that affect the central nervous system, using a custom R Language script. The presented explorative approach enabled the identification of key parameters affecting the separation and significantly reduced the time required to evaluate the comprehensive dataset from the screening experiments. Based on the data analysis results, the optimal combination of stationary phase and mobile phase composition was selected, considering retention, overall resolution, and peak shape of compounds. Additionally, compounds vulnerable to changes in selected chromatographic conditions were identified. As a complement to the presented R Language script, a web-based application ChromaFAMDeX has been developed to offer an intuitive interface that enhances the accessibility of the used statistical methods. Accompanying the publication, the R script and the link to the standalone application are provided, enabling replication and adaptation of the methodology.
Exploratory laparoscopy has been used since 1910 and is one of the new tools for diagnosing appendicitis on a routine basis. It can be looked upon as "semi-invasive" in comparison to on one hand ultrasonography/CT/MRI and on the other open laparotomy. According to the literature, mostly in retrospective studies, laparoscopy might reduce the frequencies of unnecessary appendectomies in 20-30 percent and an accuracy of diagnosis of appendicitis of 95-99 percent. This means that laparoscopy has a sensitivity of 92 percent in diagnosing acute appendicitis, including all cases of mucosal inflammation only. This means, however, that about one in 11 appendicitis is missed and it is still a question as to whether an appendix that looks normal at laparoscopy for acute right iliac fossa pain should be removed, or whether if it is not removed, there is a risk of missing an early case of appendicitis later leading to perforation and peritonitis? Mucosal inflammation obviously can never be determined in the appendix is left in place. However, a summery of available data seems to favour that it is distinctly uncommon that explorative laparoscopy misses any acute appendicitis that requires appendectomy. If there are cases of acute appendicitis not requiring appendectomy can only be known in prospective studies. A Cochran review was published in early 2002 and analysed 45 randomized trials, 39 of which had been carried out in adults, comparing both therapeutic and diagnostic outcomes of patients undergoing open or laparoscopic surgery for suspected appendicitis. Diagnostic outcomes favoured the laparoscopic approach in that both the negative appendectomy rate and "the frequency of an unestablished diagnosis" were reduced, most significantly in women in their reproductive years. However, in the conclusion the authors sounded a note of caution about the quality of some of the research data they had analysed, and recommended that more blinded studies be performed. Because there is a belief that laparoscopic appendectomy is less likely to cause intraperitoneal adhesions than open surgery, they also recommended longer follow-up studies to assess the relative incidences of obstruction due to adhesions resulting from the two techniques. Therefore, the conclusion can be drawn that in all equivocal cases laparoscopy is better than laparotomy as the initial step in year 2003. However, there is still a lack of data comparing non-invasive modern techniques such as ultrasonography and CT with laparoscopy, and the value of laparoscopy after the best available ultrasonography or CT.
- MeSH
- akutní nemoc MeSH
- apendicitida diagnóza chirurgie MeSH
- laparoskopie * MeSH
- lidé MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
Aging is an inevitable and complex biological process that is associated with a gradual decline in physiological functions and a higher disease susceptibility. Omega-3 fatty acids, particularly docosahexaenoic acid, play a crucial role in maintaining brain health and their deficiency is linked to age-related cognitive decline. Combining omega-3-rich diets with exercise may enhance cognitive function more effectively, as both share overlapping neurobiological and physiological effects. This study aimed to evaluate the effect of exercise and omega-3 fatty acid (FA) supplementation in two different doses (160 mg/kg and 320 mg/kg) on anxiety-like behavior and cognitive abilities in both adult and aged rats. Male Wistar rats (4-5- and 23-24-month-old) were randomly divided into seven groups: 3-week control supplemented with placebo without exercise, low-dose omega-3 FAs, high-dose omega-3 FAs, 7-week control supplemented with placebo without exercise, exercise-only, low-dose omega-3 FAs with exercise, and high-dose omega-3 FAs with exercise. The administered oil contained omega-3 FAs with DHA:EPA in a ratio of 1.5:1. Our results indicate that aging negatively impacts the locomotor and exploratory activity of rats. In adult rats, a low dose of omega-3 FAs reduces locomotor activity when combined with exercise while high dose of omega-3 FAs reduces anxiety-like behavior and improves recognition memory when combined with exercise. The combination of omega-3 FAs and exercise had varying impacts on behavior, suggesting a need for further research in this area to fully understand their therapeutic efficacy in the context of cognitive changes associated with aging.
- MeSH
- chování zvířat účinky léků MeSH
- kognice účinky léků MeSH
- kondiční příprava zvířat * fyziologie MeSH
- krysa rodu Rattus MeSH
- kyseliny mastné omega-3 * aplikace a dávkování farmakologie MeSH
- lokomoce účinky léků fyziologie MeSH
- pátrací chování * účinky léků MeSH
- potkani Wistar * MeSH
- potravní doplňky * MeSH
- stárnutí * psychologie účinky léků MeSH
- úzkost * prevence a kontrola MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- kyseliny mastné omega-3 * MeSH
Background and aims Association between substance use and excessive play of online games exists both in theory and research. However, no study to date examined playing online games under the influence of licit and illicit drugs. Methods We questioned a convenient online sample of 3,952 Czech online gamers on their experiences and motives of using caffeine, alcohol, tobacco, psychoactive pharmaceuticals, and illicit drugs while playing massive multiplayer online games (MMOGs). Results The results showed low prevalence of illicit drug use while playing online games. Substance use was positively associated with intensity of gaming and both addiction and engagement; psychoactive substances with stimulating effect were linked to higher engagement and gaming intensity, whereas use of sedatives was associated with higher addiction score. Substance use varied slightly with the preference of game genre. Discussion Drug use while playing appears as behavior, which is mostly not related to gaming - it concerns mostly caffeine, tobacco, alcohol, or cannabis. For some users, however, drug use was fueled by motivations toward improving their cognitive enhancement and gaming performance.
- Klíčová slova
- addiction, engagement, online gaming, substance use,
- MeSH
- dítě MeSH
- dospělí MeSH
- internet * MeSH
- lidé středního věku MeSH
- lidé MeSH
- longitudinální studie MeSH
- mladiství MeSH
- mladý dospělý MeSH
- návykové chování komplikace epidemiologie psychologie MeSH
- poruchy spojené s užíváním psychoaktivních látek komplikace epidemiologie psychologie MeSH
- prevalence MeSH
- průzkumy a dotazníky MeSH
- videohry * psychologie MeSH
- Check Tag
- dítě MeSH
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
