• Klíčová slova
• BLOOD PROTEINS *, BRONCHI/radiography *,
• MeSH
• bronchografie * MeSH
• krevní plazma * MeSH
• krevní proteiny * MeSH
• lidé MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• krevní proteiny * MeSH

Non-thermal plasma (NTP) was tested as an in vitro deactivation method on four human pathogenic dermatophytes belonging to all ecological groups including anthropophilic Trichophyton rubrum and Trichophyton interdigitale, zoophilic Arthroderma benhamiae, and geophilic Microsporum gypseum. The identification of all strains was confirmed by sequencing of ITS rDNA region (internal transcribed spacer region of ribosomal DNA). Dermatophyte spores were suspended in water or inoculated on agar plates and exposed to NTP generated by a positive or negative corona discharge, or cometary discharge. After 15 min of exposure to NTP a significant decrease in the number of surviving spores in water suspensions was observed in all species. Complete spore inactivation and thus decontamination was observed in anthropophilic species after 25 min of exposure. Similarly, a significant decrease in the number of surviving spores was observed after 10-15 min of exposure to NTP on the surface of agar plates with full inhibition after 25 min in all tested species except of M. gypseum. Although the sensitivity of dermatophytes to the action of NTP appears to be lower than that of bacteria and yeast, our results suggest that NTP has the potential to be used as an alternative treatment strategy for dermatophytosis and could be useful for surface decontamination in clinical practice.

• Klíčová slova
• Cometary discharge;, Corona discharge;, Decontamination of surfaces;, Low-temperature plasma;, Microsporum;, Trichophyton,
• MeSH
• Arthrodermataceae účinky léků   genetika   růst a vývoj   účinky záření   MeSH
• lidé MeSH
• plazmové plyny toxicita   MeSH
• tinea mikrobiologie   MeSH
• ultrafialové záření MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• plazmové plyny MeSH

Coenzyme Q10 (CoQ10) levels in human plasma were determined by high-performance liquid chromatography (HPLC) with UV detection. CoQ10 was dissociated from lipoproteins by methanol and subsequently cleaned-up on silica gel and octadecyl silica solid-phase extraction cartridges. HPLC separation was performed on a C18 reversed-phase column. The methanol-hexane mobile phase provided a greater possibility of separation procedure adjustment allowing the shortest possible elution time without loss of resolution than a two-alcohol mobile phase. Quantitation was based on the peak heights using a standard addition method. The lower limit of detection was 8 ng on-column, corresponding to 90 micrograms ubiquinone per litre of plasma in an actual sample. Thirty-one randomly selected plasma samples from apparently healthy, 18 to 56-year-old individuals (males and females) were analyzed for total CoQ10. The average level in these subjects was 0.47 +/- 0.18 mg/l with the range of 0.26-1.03 mg/l. The method was also applied to the determination of ubiquinone plasma level changes in one healthy volunteer over a period of one month and after oral intake of CoQ10.

• MeSH
• dospělí MeSH
• indikátory a reagencie MeSH
• kalibrace MeSH
• krevní plazma chemie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• referenční hodnoty MeSH
• spektrofotometrie ultrafialová MeSH
• ubichinon krev   MeSH
• vysokoúčinná kapalinová chromatografie MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• klinické zkoušky MeSH
• práce podpořená grantem MeSH
• randomizované kontrolované studie MeSH
• Názvy látek
• indikátory a reagencie MeSH
• ubichinon MeSH

A new hydrophilic interaction liquid chromatography - mass spectrometry method is developed for low-abundant phospholipids and sphingolipids in human plasma and serum. The optimized method involves the Cogent Silica type C hydride column, the simple sample preparation by protein precipitation, and the removal of highly abundant lipid classes using the postcolumn valve directed to waste during two elution windows. The method allows a highly confident and sensitive identification of low-abundant lipid classes in human plasma (246 lipid species from 24 lipid subclasses) based on mass accuracy and retention dependencies in both polarity modes. The method is validated for quantitation using two internal standards (if available) for each lipid class and applied to human plasma and serum samples obtained from patients with pancreatic ductal adenocarcinoma (PDAC), healthy controls, and NIST SRM 1950. Multivariate data analysis followed by various statistical projection methods is used to determine the most dysregulated lipids. Significant downregulation is observed for lysophospholipids with fatty acyl composition 16:0, 18:0, 18:1, and 18:2. Distinct trends are observed for phosphatidylethanolamines (PE) in relation to the bonding type of fatty acyls, where most PE with acyl bonds are upregulated, while ether/plasmenyl PE are downregulated. For the sphingolipid category, sphingolipids with very long N-acyl chains are downregulated, while sphingolipids with shorter N-acyl chains were upregulated in PDAC. These changes are consistently observed for various classes of sphingolipids, ranging from ceramides to glycosphingolipids, indicating a possible metabolic disorder in ceramide biosynthesis caused by PDAC.

• Klíčová slova
• Human plasma, Human serum, Hydrophilic interaction liquid chromatography, Lipidomics, Liquid chromatography, Mass spectrometry, Pancreatic cancer,
• MeSH
• ceramidy MeSH
• krevní plazma chemie   MeSH
• lidé MeSH
• nádory slinivky břišní * MeSH
• sérum MeSH
• sfingolipidy * analýza   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• ceramidy MeSH
• sfingolipidy * MeSH

OBJECTIVE: A new interleukin-6 (IL-6)-dependent plasma cell leukemia cell line UHKT-944 was established from bone marrow cells derived from a 55-yr-old man with plasma cell leukemia. RESULTS: The cell line possesses phenotypic characteristics of plasma cells including the production of a monoclonal immunoglobulin IgA1-kappa. VH3-9 region of IgVH genes was rearranged and somatically hypermutated. The UHKT-944 cells were found to be negative for most of tested B-cell, T-cell, and myeloid markers. According to cytogenetic analysis, the cells were classified as near tetraploid with several numerical and structural abnormalities including the t(14;20) involving IgH locus. CONCLUSION: The established permanent plasma cell leukemia cell line is a suitable model for the study of cellular and molecular mechanisms of pathogenesis of this rare malignant disease.

• Klíčová slova
• human plasma cell line, interleukin-6 (IL-6), multiple myeloma, plasma cell, plasma cell leukemia,
• MeSH
• biologické markery MeSH
• cytogenetické vyšetření MeSH
• imunofenotypizace MeSH
• imunoglobuliny genetika   metabolismus   MeSH
• lidé středního věku MeSH
• lidé MeSH
• nádorové buněčné linie MeSH
• plazmocelulární leukemie diagnóza   metabolismus   patologie   MeSH
• proliferace buněk MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH
• práce podpořená grantem MeSH
• Názvy látek
• biologické markery MeSH
• imunoglobuliny MeSH

• Klíčová slova
• BLOOD BANKS *, BLOOD TRANSFUSION *,
• MeSH
• krevní banky * MeSH
• krevní plazma * MeSH
• krevní transfuze * MeSH
• lidé MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

The growing application of materials containing TiO2 particles has led to an increased risk of human exposure, while a gap in knowledge about the possible adverse effects of TiO2 still exists. In this work, TiO2 particles of rutile, anatase, and their commercial mixture were exposed to various environments, including simulated gastric fluids and human blood plasma (both representing in vivo conditions), and media used in in vitro experiments. Simulated body fluids of different compositions, ionic strengths, and pH were used, and the impact of the absence or presence of chosen enzymes was investigated. The physicochemical properties and agglomeration of TiO2 in these media were determined. The time dependent agglomeration of TiO2 related to the type of TiO2, and mainly to the type and composition of the environment that was observed. The presence of enzymes either prevented or promoted TiO2 agglomeration. TiO2 was also observed to exhibit concentration-dependent cytotoxicity. This knowledge about TiO2 behavior in all the abovementioned environments is critical when TiO2 safety is considered, especially with respect to the significant impact of the presence of proteins and size-related cytotoxicity.

• Klíčová slova
• TiO2 particles, agglomeration, plasma, proteins, simulated gastric fluids,
• MeSH
• buněčné linie MeSH
• dárci krve MeSH
• fibroblasty účinky léků   metabolismus   MeSH
• koncentrace vodíkových iontů MeSH
• kovové nanočástice škodlivé účinky   chemie   MeSH
• krevní plazma metabolismus   MeSH
• krystalizace MeSH
• kultivační média metabolismus   MeSH
• lidé MeSH
• myši MeSH
• osmolární koncentrace MeSH
• povrchové vlastnosti MeSH
• sliny metabolismus   MeSH
• titan škodlivé účinky   chemie   metabolismus   MeSH
• velikost částic MeSH
• viabilita buněk účinky léků   MeSH
• voda metabolismus   MeSH
• zdraví dobrovolníci pro lékařské studie MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• kultivační média MeSH
• titan MeSH
• titanium dioxide MeSH Prohlížeč
• voda MeSH

Currently, one of the most promising treatments of lipopolysaccharides (LPS)-induced sepsis is based on hemofiltration. Nevertheless, proteins rapidly adsorbed on the artificial surface of membranes which leads to activation of coagulation impairing effective scavenging of the endotoxins. To overcome this challenge, we designed polymer-brush-coated microparticles displaying antifouling properties and functionalized them with polymyxin B (PMB) to specifically scavenge LPS the most common endotoxin. Poly[( N-(2-hydroxypropyl) methacrylamide)- co-(carboxybetaine methacrylamide)] brushes were grafted from poly(glycidyl methacrylate) microparticles using photoinduced single-electron transfer living radical polymerization (SET-LRP). Notably, only parts-per-million of copper catalyst were necessary to achieve brushes able to repel adsorption of proteins from blood plasma. The open porosity of the particles, accessible to polymerization, enabled us to immobilize sufficient PMB to selectively scavenge LPS from blood plasma.

• MeSH
• adsorpce MeSH
• akrylamidy metabolismus   MeSH
• biokompatibilní potahované materiály farmakologie   MeSH
• bioznečištění prevence a kontrola   MeSH
• epoxidové sloučeniny metabolismus   MeSH
• krevní plazma metabolismus   MeSH
• lidé MeSH
• lipopolysacharidy metabolismus   MeSH
• methakryláty metabolismus   MeSH
• polymerizace účinky léků   MeSH
• polymery chemie   MeSH
• polymyxin B farmakologie   MeSH
• povrchové vlastnosti účinky léků   MeSH
• proteiny metabolismus   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• akrylamidy MeSH
• biokompatibilní potahované materiály MeSH
• epoxidové sloučeniny MeSH
• glycidyl methacrylate MeSH Prohlížeč
• lipopolysacharidy MeSH
• methacrylamide MeSH Prohlížeč
• methakryláty MeSH
• polymery MeSH
• polymyxin B MeSH
• proteiny MeSH

• Klíčová slova
• BLOOD PROTEINS/chemistry *, PENICILLIN/blood *,
• MeSH
• jaderné receptory - podrodina 4, skupina A, člen 2 * MeSH
• krevní plazma * MeSH
• krevní proteiny chemie   MeSH
• lidé MeSH
• peniciliny krev   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• jaderné receptory - podrodina 4, skupina A, člen 2 * MeSH
• krevní proteiny MeSH
• NR4A2 protein, human MeSH Prohlížeč
• peniciliny MeSH

Raman optical activity (ROA) is inherently sensitive to the secondary structure of biomolecules, which makes it a method of interest for finding new approaches to clinical applications based on blood plasma analysis, for instance the diagnostics of several protein-misfolding diseases. Unfortunately, real blood plasma exhibits strong background fluorescence when excited at 532 nm; hence, measuring the ROA spectra appears to be impossible. Therefore, we established a suitable method using a combination of kinetic quenchers, filtering, photobleaching, and a mathematical correction of residual fluorescence. Our method reduced the background fluorescence approximately by 90%, which allowed speedup for each measurement by an average of 50%. In addition, the signal-to-noise ratio was significantly increased, while the baseline distortion remained low. We assume that our method is suitable for the investigation of human blood plasma by ROA and may lead to the development of a new tool for clinical diagnostics.

• MeSH
• fluorescence MeSH
• krevní plazma chemie   MeSH
• lidé MeSH
• optická otáčivost MeSH
• Ramanova spektroskopie přístrojové vybavení   metody   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH