OBJECTIVES: Our objective was to compare the measurement of residual white blood cell (rWBC) and residual red blood cell (rRBC) counts in blood products using the XN Blood Bank mode and the laboratory standard operating procedures for manual counts. In addition, to compare the whole blood complete blood count (CBC) values of blood donors and the quality of blood products using the Sysmex XN analyser versus the XS-1000i analyser. MATERIALS AND METHODS: For blood donors, 190 samples from blood or apheresis donors were analysed on both the Sysmex XS-1000i and XN-1000 analysers and the mean values of six CBC parameters were compared: the white blood cell count (WBC), the red blood cell count (RBC), haemoglobin (HGB), haematocrit (HCT), the mean corpuscular volume (MCV), the platelet count (PLT). For blood products, 164 samples were collected: 13 Plasma products - whole blood, 9 Plasma products - apheresis, 36 RBC concentrates - whole blood, 30 PLT concentrates - buffy coats, 36 PLT concentrates - buffy coats - pooled and 55 PLT concentrates - apheresis. RESULTS: All CBC parameters of the blood donors tested showed similar performance, with excellent correlation coefficients (r) ranging from 0.821 to 0.995. The majority of the blood products did not have a quantifiable number of residual cells, meaning the number of rWBC and rRBC, if present, was below the limit of quantitation (LoQ) of the different methods. rWBC were detected by Blood Bank mode in Plasma products - whole blood with a mean rWBC of 0.012 × 109 /L and in PLT concentrates - buffy coats with a mean rWBC of 0.19 × 109 /L. The correlation coefficient in both analysers for all three parameters (HGB, HCT, RBC) in RBC concentrates - whole blood was excellent, ranging from 0.95 to 0.99. For platelet count, r ranged from 0.98 to 0.99. CONCLUSION: The XN-Series analyser, equipped with a Blood Bank mode, demonstrated reliable performance when used for blood donor evaluation, rWBC enumeration and measurement of end blood products.
- Klíčová slova
- XN analyser, blood bank mode, blood donor, quality control, residual red blood cells, residual white blood cells, transfusion product,
- MeSH
- dárci krve * MeSH
- erytrocyty MeSH
- krevní banky * MeSH
- krevní obraz metody MeSH
- lidé MeSH
- počet trombocytů MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
According to the Standards of the World Marrow Donor Association (WMDA), unrelated stem cell donor registries and donor centers are responsible for compliance of their collection and apheresis centers with these Standards. To ensure high stem cell product quality and high standards for safety and satisfaction of voluntary unrelated stem cell donors, we here present guidelines for audits of collection and apheresis centers that can be used by new and established donor registries, as well as by collection centers in preparation of audits. We define the general requirements and recommendations for collaboration with the collection and apheresis centers and define critical procedures for the collection of the stem cell product, such as information session, medical assessment, product collection, quality controls, product handover for transportation, and donor follow-up. The specific guidelines are accompanied by detailed checklists and forms that can be found in Supplementary Information and may be used during an initial or follow-up on-site or paper-based audit.
- MeSH
- audit řízení MeSH
- dárci tkání MeSH
- krevní banky normy MeSH
- lidé MeSH
- registrace normy MeSH
- řízení kvality * MeSH
- separace krevních složek normy MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- směrnice MeSH
- MeSH
- banky biologického materiálu zákonodárství a právo organizace a řízení MeSH
- databáze faktografické MeSH
- krevní banky MeSH
- lidé MeSH
- odběr biologického vzorku metody MeSH
- rozvoj plánování MeSH
- sběr dat MeSH
- univerzity MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
Current RFID technology deployment is limited by safety, procedural and physical limitations in healthcare field. It is important to define and ensure safe operation of technologies without actual deployment in real operation. Potential problems could arise due to the consequences of technical and physical characteristics of RFID technology and its improper location. This article deals with manipulation of blood products and the definition of suitable places for radio identification. Each suitable place must undergo laboratory experiments and tests. The results can provide a convenient base for defining efficient and safe deployment of RFID technology in Blood Centers with substantial financial savings for Czech healthcare.
The authors investigated the effectiveness of ordering of blood for elective surgery. Analysis of a group of 333 operated patients revealed that blood was administered only in 9% of the operations, i.e when compared with the number ordered the ratio ordered: issued was 13:1. Based on their own experience the authors present the possibility how to improve the blood ordering system from the surgical as well as blood bank activity aspect.
- MeSH
- elektivní chirurgické výkony * MeSH
- krevní banky * MeSH
- krevní transfuze MeSH
- lidé MeSH
- nemocniční komunikační systémy * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
Serum samples were tested for antibodies against polio virus and measles virus from two serum banks after having been stored under different conditions for several years. The results are compared with those ones obtained immediately after sampling. Despite of small increases of antibody-negative samples and of small decreases of the titres the suitability of the samples stored could be shown. Special attention should be given to the establishment, e.g. in the framework of Expanded Programme on Immunization of the World Health Organization and in order to study new pathogens unknown to date, and the technical preconditions for that should be planed.
- MeSH
- časové faktory MeSH
- krevní banky * MeSH
- kryoprezervace * MeSH
- lidé MeSH
- odběr vzorku krve MeSH
- poliomyelitida krev epidemiologie imunologie MeSH
- protilátky virové krev MeSH
- séroepidemiologické studie MeSH
- spalničky krev epidemiologie imunologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
- Geografické názvy
- Československo epidemiologie MeSH
- Keňa epidemiologie MeSH
- Německo epidemiologie MeSH
- Nigérie epidemiologie MeSH
- Togo epidemiologie MeSH
- Názvy látek
- protilátky virové MeSH
The following trends aim to a more efficient exploitation of packed red blood cells (PRBC): 1. Improvement of the operative distribution of PRBCs for transfusions before expiration. 2. Prolongation of the expiration time by monitoring the biochemical and physical processes during banking. Maintenance of native hemoglobin and restoration or substitution of substances involved in transport of energy and of oxygen are of utmost importance. Enzymic conversion of RBCs of blood group A, B to 0 is not supposed to leave laboratory scale soon. While cryo-conservation of RBCs with glycerine is known, freeze-drying of PRBCs remains a speculation. 3. Use of PRBCs after expiration as a raw material for products applicable in medicine and biochemistry. Stroma-free hemoglobin variants (SFH) are known as effective infusable oxygen carriers in experimental animal models. However, there is little convincing evidence on the metabolism and innocuity of SFH variants in human organism. Therefore, systemic infusion of SFH solutions is not yet acceptable to clinicians even in emergency situations. On the other hand, a broader use of SFH and its variants is anticipated and regarded as prospective in organ perfusion, cardioplegy and transplantation as well as in analytical biochemistry.
- MeSH
- časové faktory MeSH
- erytrocyty * MeSH
- hemoglobiny MeSH
- konzervace krve MeSH
- krevní banky * normy MeSH
- krevní transfuze MeSH
- lidé MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
- Názvy látek
- hemoglobin, stroma free MeSH Prohlížeč
- hemoglobiny MeSH
- MeSH
- konzervace krve MeSH
- krevní banky MeSH
- krevní transfuze * normy MeSH
- lidé MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Německo východní MeSH
- MeSH
- erytrocyty * MeSH
- hematokrit MeSH
- krevní banky MeSH
- krevní transfuze * MeSH
- lidé MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- Geografické názvy
- Československo MeSH
- MeSH
- antigeny krevních skupin MeSH
- dárci krve MeSH
- konzervace krve MeSH
- krevní banky * MeSH
- krevní transfuze * MeSH
- počítače MeSH
- státní lékařství MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Československo MeSH
- Názvy látek
- antigeny krevních skupin MeSH
