Metabolic phenotypes of cancer cells are heterogeneous and flexible as a tumor mass is a hurriedly evolving system capable of constant adaptation to oxygen and nutrient availability. The exact type of cancer metabolism arises from the combined effects of factors intrinsic to the cancer cells and factors proposed by the tumor microenvironment. As a result, a condition termed oncogenic metabolic symbiosis in which components of the tumor microenvironment (TME) promote tumor growth often occurs. Understanding how oncogenic metabolic symbiosis emerges and evolves is crucial for perceiving tumorigenesis. The process by which tumor cells reprogram their TME involves many mechanisms, including changes in intercellular communication, alterations in metabolic phenotypes of TME cells, and rearrangement of the extracellular matrix. It is possible that one molecule with a pleiotropic effect such as Caveolin-1 may affect many of these pathways. Here, we discuss the significance of Caveolin-1 in establishing metabolic symbiosis in TME.

• Klíčová slova
• Caveolin-1, cancer, cancer-associated fibroblast, glycolysis, metabolic symbiosis, metabolism, tumor microenvironment,
• MeSH
• kaveolin 1 metabolismus   MeSH
• lidé MeSH
• mezibuněčná komunikace MeSH
• nádorové mikroprostředí MeSH
• nádory metabolismus   MeSH
• regulace genové exprese u nádorů MeSH
• symbióza MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH
• Názvy látek
• CAV1 protein, human MeSH Prohlížeč
• kaveolin 1 MeSH

The paper presents an extension of Pinch Analysis and namely, Total Site Process Integration. It benefits from up to date developments and introduction of Total EcoSite Integration for urban and industrial symbiosis. An important development is Pinch Analysis for Solid Waste Integration which is a crucial step for the symbiosis in a circular economy. As the potential EcoSites are usually extensive and cover various units, a methodology based on clusters has been used. The solution has been supported by graphical tools using the analogy with already implemented extensions of Pinch Analysis. The results of a demonstration case study revealed the potential of the novel approach. The identified integrated design increased the energy recovered from the solid waste by 11.39 MWh/d and diverted 2 t/d of the waste from the landfill, benefiting both the urban and industrial site. The proposed approach is also capable of minimising the requirement of energy-intensive thermal drying for waste whenever the process allowed, subsequently offer a solution with lower environmental footprint and cost. For future work, a even more comprehensive case study can be conducted by considering the other forms of the waste, recovery process and drying approaches.

• Klíčová slova
• Process Integration, Solid Waste Integration, Total EcoSite Integration, Urban and industrial symbiosis, Waste recovery,
• MeSH
• nakládání s odpady * MeSH
• odpadky - odstraňování * MeSH
• průmysl MeSH
• skládková zařízení MeSH
• symbióza MeSH
• tuhý odpad analýza   MeSH
• životní prostředí MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• tuhý odpad MeSH

Arbuscular mycorrhizal fungi (AMF) colonize the roots of numerous aquatic and wetland plants, but the establishment and functioning of mycorrhizal symbiosis in submerged habitats have received only little attention. Three pot experiments were conducted to study the interaction of isoetid plants with native AMF. In the first experiment, arbuscular mycorrhizal (AM) symbiosis did not establish in roots of Isoëtes echinospora and I. lacustris, while Littorella uniflora roots were highly colonized. Shoot and root biomass of L. uniflora were, however, not affected by AMF inoculation, and only one of nine AMF isolates significantly increased shoot P concentration. In the second experiment, we compared colonization by three Glomus tetrastratosum isolates of different cultivation history and origin (aquatic versus terrestrial) and their effects on L. uniflora growth and phosphorus nutrition under submerged versus terrestrial conditions. The submerged cultivation considerably slowed, but did not inhibit mycorrhizal root colonization, regardless of isolate identity. Inoculation with any AMF isolate improved plant growth and P uptake under terrestrial, but not submerged conditions. In the final experiment, we compared the communities of AMF established in two cultivation regimes of trap cultures with lake sediments, either submerged on L. uniflora or terrestrial on Zea mays. After 2-year cultivation, we did not detect a significant effect of cultivation regime on AMF community composition. In summary, although submerged conditions do not preclude the development of functional AM symbiosis, the contribution of these symbiotic fungi to the fitness of their hosts seems to be considerably less than under terrestrial conditions.

• Klíčová slova
• Aquatic plants, Arbuscular mycorrhizal symbiosis, Isoetids, Isoëtes, Littorella uniflora, Submerged roots,
• MeSH
• biomasa MeSH
• Glomeromycota * MeSH
• houby MeSH
• kořeny rostlin MeSH
• mykorhiza * MeSH
• symbióza MeSH
• Publikační typ
• časopisecké články MeSH

Insects that live exclusively on vertebrate blood utilize symbiotic bacteria as a source of essential compounds, e.g., B vitamins. In louse flies, the most frequent symbiont originated in genus Arsenophonus, known from a wide range of insects. Here, we analyze genomic traits, phylogenetic origins, and metabolic capacities of 11 Arsenophonus strains associated with louse flies. We show that in louse flies, Arsenophonus established symbiosis in at least four independent events, reaching different stages of symbiogenesis. This allowed for comparative genomic analysis, including convergence of metabolic capacities. The significance of the results is twofold. First, based on a comparison of independently originated Arsenophonus symbioses, it determines the importance of individual B vitamins for the insect host. This expands our theoretical insight into insect-bacteria symbiosis. The second outcome is of methodological significance. We show that the comparative approach reveals artifacts that would be difficult to identify based on a single-genome analysis.

• Klíčová slova
• bacterial symbiosis, coevolution, genome evolution, hematophagy,
• MeSH
• Anoplura * MeSH
• Bacteria MeSH
• Diptera * mikrobiologie   MeSH
• Enterobacteriaceae MeSH
• fylogeneze MeSH
• Gammaproteobacteria * genetika   MeSH
• hmyz MeSH
• symbióza MeSH
• vitamin B komplex * MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• vitamin B komplex * MeSH

• Klíčová slova
• AZOTOBACTER *, SYMBIOSIS *,
• MeSH
• Azotobacter * MeSH
• symbióza * MeSH
• Publikační typ
• časopisecké články MeSH

Anthropogenic nitrogen (N) enrichment can have complex effects on plant communities. In low-nutrient, primary successional systems such as sand dunes, N enrichment may alter the trajectory of plant community assembly or the dominance of foundational, ecosystem-engineering plants. Predicting the consequences of N enrichment may be complicated by plant interactions with microbial symbionts because increases in a limiting resource, such as N, could alter the costs and benefits of symbiosis. To evaluate the direct and interactive effects of microbial symbiosis and N addition on plant succession, we established a long-term field experiment in Michigan, USA, manipulating the presence of the symbiotic fungal endophyte Epichloë amarillans in Ammophila breviligulata, a dominant ecosystem-engineering dune grass species. From 2016 to 2020, we implemented N fertilization treatments (control, low, high) in a subset of the long-term experiment. N addition suppressed the accumulation of plant diversity over time mainly by reducing species richness of colonizing plants. However, this suppression occurred only when the endophyte was present in Ammophila. Although Epichloë enhanced Ammophila tiller density over time, N addition did not strongly interact with Epichloë symbiosis to influence vegetative growth of Ammophila. Instead, N addition directly altered plant community composition by increasing the abundance of efficient colonizers, especially C4 grasses. In conclusion, hidden microbial symbionts can alter the consequences of N enrichment on plant primary succession.

• Klíčová slova
• Ammophila, Epichloë, Fungi, Mutualism, Nitrogen deposition,
• MeSH
• ekosystém * MeSH
• endofyty MeSH
• lipnicovité MeSH
• rostliny MeSH
• symbióza * MeSH
• životní prostředí MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

Cycads are ancient seed plants (gymnosperms) that emerged by the early Permian. Although they were common understory flora and food for dinosaurs in the Mesozoic, their abundance declined markedly in the Cenozoic. Extant cycads persist in restricted populations in tropical and subtropical habitats and, with their conserved morphology, are often called 'living fossils.' All surviving taxa receive nitrogen from symbiotic N2-fixing cyanobacteria living in modified roots, suggesting an ancestral origin of this symbiosis. However, such an ancient acquisition is discordant with the abundance of cycads in Mesozoic fossil assemblages, as modern N2-fixing symbioses typically occur only in nutrient-poor habitats where advantageous for survival. Here, we use foliar nitrogen isotope ratios-a proxy for N2 fixation in modern plants-to probe the antiquity of the cycad-cyanobacterial symbiosis. We find that fossilized cycad leaves from two Cenozoic representatives of extant genera have nitrogen isotopic compositions consistent with microbial N2 fixation. In contrast, all extinct cycad genera have nitrogen isotope ratios that are indistinguishable from co-existing non-cycad plants and generally inconsistent with microbial N2 fixation, pointing to nitrogen assimilation from soils and not through symbiosis. This pattern indicates that, rather than being ancestral within cycads, N2-fixing symbiosis arose independently in the lineages leading to living cycads during or after the Jurassic. The preferential survival of these lineages may therefore reflect the effects of competition with angiosperms and Cenozoic climatic change.

• MeSH
• cykasy MeSH
• dusík MeSH
• izotopy dusíku MeSH
• sinice * MeSH
• symbióza * MeSH
• zkameněliny MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• dusík MeSH
• izotopy dusíku MeSH

Many insects host vertically transmitted microbes, which can confer benefits to their hosts but are costly to maintain and regulate. A key feature of these symbioses is variation: for example, symbiont density can vary among host and symbiont genotypes. However, the evolutionary forces maintaining this variation remain unclear. We studied variation in symbiont density using the pea aphid (Acyrthosiphon pisum) and the bacterium Regiella insecticola, a symbiont that can protect its host against fungal pathogens. We found that relative symbiont density varies both between two Regiella phylogenetic clades and among aphid "biotypes." Higher density symbiont infections are correlated with stronger survival costs, but variation in density has little effect on the protection Regiella provides against fungi. Instead, we found that in some aphid genotypes, a dramatic decline in symbiont density precedes the loss of a symbiont infection. Together, our data suggest that the optimal density of a symbiont infection is likely different from the perspective of aphid and microbial fitness. Regiella might prevent loss by maintaining high within-host densities, but hosts do not appear to benefit from higher symbiont numbers and may be advantaged by losing costly symbionts in certain environments. The standing variation in symbiont density observed in natural populations could therefore be maintained by antagonistic coevolutionary interactions between hosts and their symbiotic microbes.

• Klíčová slova
• density, facultative symbiont, intraspecific variation, pea aphid, symbiosis,
• MeSH
• Enterobacteriaceae genetika   MeSH
• fylogeneze MeSH
• houby MeSH
• mšice * genetika   MeSH
• symbióza * MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Research Support, U.S. Gov't, Non-P.H.S. MeSH

Arbuscular mycorrhizal fungi (AMF) (subphylum Glomeromycotina)1 are among the most prominent symbionts and form the Arbuscular Mycorrhizal symbiosis (AMS) with over 70% of known land plants.2,3 AMS allows plants to efficiently acquire poorly soluble soil nutrients4 and AMF to receive photosynthetically fixed carbohydrates. This plant-fungus symbiosis dates back more than 400 million years5 and is thought to be one of the key innovations that allowed the colonization of lands by plants.6 Genomic and genetic analyses of diverse plant species started to reveal the molecular mechanisms that allowed the evolution of this symbiosis on the host side, but how and when AMS abilities emerged in AMF remain elusive. Comparative phylogenomics could be used to understand the evolution of AMS.7,8 However, the availability of genome data covering basal AMF phylogenetic nodes (Archaeosporales, Paraglomerales) is presently based on fragmentary protein coding datasets.9Geosiphon pyriformis (Archaeosporales) is the only fungus known to produce endosymbiosis with nitrogen-fixing cyanobacteria (Nostoc punctiforme) presumably representing the ancestral AMF state.10-12 Unlike other AMF, it forms long fungal cells ("bladders") that enclose cyanobacteria. Once in the bladder, the cyanobacteria are photosynthetically active and fix nitrogen, receiving inorganic nutrients and water from the fungus. Arguably, G. pyriformis represents an ideal candidate to investigate the origin of AMS and the emergence of a unique endosymbiosis. Here, we aimed to advance knowledge in these questions by sequencing the genome of G. pyriformis, using a re-discovered isolate.

• Klíčová slova
• Geosiphon pyriformis, Nostoc Punctiforme, binning, bladders, cyanobacteria, endocyanosis, homokaryon, metagenome, symbiosis, transposon,
• MeSH
• fixace dusíku MeSH
• fylogeneze MeSH
• genom fungální * MeSH
• houby genetika   MeSH
• mykorhiza * genetika   MeSH
• rostliny * mikrobiologie   MeSH
• sinice MeSH
• symbióza genetika   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Research Support, U.S. Gov't, Non-P.H.S. MeSH

Despite decades of intensive research (especially from 1970s to 1990s), the ericoid mycorrhizal (ErM) hair root is still largely terra incognita and this simplified guide is intended to revive and promote the study of its mycobiota. Basic theoretical knowledge on the ErM symbiosis is summarized, followed by practical advices on Ericaceae root sample collection and handling, microscopic observations and photo-documentation of root fungal colonization, mycobiont isolation, maintenance and identification and resynthesis experiments with ericoid plants. The necessity of a proper selection of the root material and its surface sterilization prior to mycobiont isolation is stressed, together with the need of including suitable control treatments in inoculation experiments. The culture-dependent approach employing plating of single short (~ 2 mm) hair root segments on nutrient media is substantiated as a useful tool for characterization of Ericaceae root-associated fungal communities; it targets living mycelium and provides metabolically active cultures that can be used in physiological experiments and taxonomic studies, thus providing essential reference material for culture-independent approaches. On the other hand, it is stressed that not every mycobiont isolated from an ericoid hair root necessarily represent an ErM fungus. Likewise, not every intracellular hyphal coil formed in the Ericaceae rhizodermis necessarily represents the ErM symbiosis. Taxonomy of the most important ericoid mycobionts is updated, mutualism in the ErM symbiosis is briefly discussed from the mycobiont perspective, and some interesting lines of possible future research are highlighted.

• Klíčová slova
• Culture-dependent approach, Ericaceae, Ericoid mycorrhizal fungal diversity, In vitro resynthesis, Isolate identification, Microscopy, Mycobiont isolation, Plating of surface-sterilized root segments,
• MeSH
• Ericaceae * MeSH
• kořeny rostlin MeSH
• mykorhiza * MeSH
• rostliny MeSH
• symbióza MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH