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Piezoelectric immunosensor for the direct and rapid detection of Francisella tularensis

Miroslav Pohanka, Petr Skládal

. 2007 ; 52 (4) : 325-330.

Language English Country Czech Republic

Persistent link   https://www.medvik.cz/link/bmc07503485

A novel immunosensing device based on a piezoelectric sensor for direct detection of the biological warfare agent Francisella tularensis was developed. This sensor includes mouse polyclonal antibody immobilized in a layer of protein A covalently linked to the gold electrode of the sensor. The immunosensor is able to detect F. tularensis with the limit of detection 10(5) CFU/mL with a typical measuring cycle > 5 min. The sensor was successfully evaluated for rapid detection of F. tularensis spikes in drinking water and milk; no deterioration of sensitivity in comparison with buffer solutions was observed. The proposed concept of a rapid measurement of microbial agents seems to be promising for evaluation of samples after short pre-cultivation enrichment.

References provided by Crossref.org

Grant č. LC06030 Ministerstvo školství, mládeže a tělovýchovy

Bibliography, etc.

Lit.: 21

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