• Something wrong with this record ?

Casitone-dependent transcriptional regulation of the prtP and prtM genes in the natural isolate Lactobacillus paracasei subsp. paracasei

I. Paštar, J. Begović, J. Lozo, L. Topisirović, N. Golić

. 2007 ; 52 (6) : 577-584.

Language English Country Czech Republic

Persistent link   https://www.medvik.cz/link/bmc07507021

The prtP-prtM intergenic region of Lactobacillus paracasei subsp. paracasei BGHN 14 was cloned and sequenced. The nucleotide sequence of the prtP-prtM intergenic region in BGHN 14, containing divergently orientated P(prtP) and P(prtP) promoters, was shorter by 35 bp in comparison with that in lactococci. The nucleotide sequence involved in casitone-dependent transcriptional regulation of the lactococcal prt genes was not found in the BGHN14. The activity of P(prtM) in L. lactis NZ9000 was very low and insignificantly changed in the presence of casitone, whereas P(prtP) was completely inactive. When L. casei ATCC393(T) was used as host, both P(prtP) and P(prtM) were active and strongly regulated by casitone. The results strongly indicate that the mechanisms of the casitone-dependent regulation of the prt genes in BGHN14 and lactococci are different.

Bibliography, etc.

Lit.: 29

000      
02809naa 2200433 a 4500
001      
bmc07507021
003      
CZ-PrNML
005      
20111210122801.0
008      
080725s2007 xr e eng||
009      
AR
040    __
$a ABA008 $b cze $c ABA008 $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xr
100    1_
$a Paštar, I.
245    10
$a Casitone-dependent transcriptional regulation of the prtP and prtM genes in the natural isolate Lactobacillus paracasei subsp. paracasei / $c I. Paštar, J. Begović, J. Lozo, L. Topisirović, N. Golić
314    __
$a Institute of Molecular Genetics and Genetic Engineering, Belgrade
504    __
$a Lit.: 29
520    9_
$a The prtP-prtM intergenic region of Lactobacillus paracasei subsp. paracasei BGHN 14 was cloned and sequenced. The nucleotide sequence of the prtP-prtM intergenic region in BGHN 14, containing divergently orientated P(prtP) and P(prtP) promoters, was shorter by 35 bp in comparison with that in lactococci. The nucleotide sequence involved in casitone-dependent transcriptional regulation of the lactococcal prt genes was not found in the BGHN14. The activity of P(prtM) in L. lactis NZ9000 was very low and insignificantly changed in the presence of casitone, whereas P(prtP) was completely inactive. When L. casei ATCC393(T) was used as host, both P(prtP) and P(prtM) were active and strongly regulated by casitone. The results strongly indicate that the mechanisms of the casitone-dependent regulation of the prt genes in BGHN14 and lactococci are different.
650    _2
$a Lactobacillus $x genetika $x růst a vývoj $7 D007778
650    _2
$a regulace genové exprese $x imunologie $x účinky záření $7 D005786
650    _2
$a sekvenční analýza $x metody $x využití $7 D017421
650    _2
$a kaseiny $x genetika $7 D002364
650    _2
$a plazmidy $x genetika $7 D010957
650    _2
$a elektroforéza v polyakrylamidovém gelu $x využití $7 D004591
650    _2
$a polymerázová řetězová reakce $x využití $7 D016133
650    _2
$a promotorové oblasti (genetika) $x imunologie $x účinky záření $7 D011401
650    _2
$a financování organizované $x využití $7 D005381
700    1_
$a Begović, J.
700    1_
$a Lozo, J.
700    1_
$a Topisirović, L.
700    1_
$a Golić, N.
773    0_
$w MED00011005 $t Folia microbiologica $g Roč. 52, č. 6 (2007), s. 577-584 $x 0015-5632
910    __
$a ABA008 $b A 2403 $c 561 $y 1
990    __
$a 20080725124903 $b ABA008
991    __
$a 20080728150100 $b ABA008
999    __
$a ok $b bmc $g 622629 $s 475062
BAS    __
$a 3
BMC    __
$a 2007 $b 52 $c 6 $d 577-584 $i 0015-5632 $m Folia microbiologica $x MED00011005
LZP    __
$a 2008-11/mkal

Find record

Citation metrics

Archiving options