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Casitone-dependent transcriptional regulation of the prtP and prtM genes in the natural isolate Lactobacillus paracasei subsp. paracasei
I. Paštar, J. Begović, J. Lozo, L. Topisirović, N. Golić
Jazyk angličtina Země Česko
- MeSH
- elektroforéza v polyakrylamidovém gelu využití MeSH
- financování organizované využití MeSH
- kaseiny genetika MeSH
- Lactobacillus genetika růst a vývoj MeSH
- plazmidy genetika MeSH
- polymerázová řetězová reakce využití MeSH
- promotorové oblasti (genetika) imunologie účinky záření MeSH
- regulace genové exprese imunologie účinky záření MeSH
- sekvenční analýza metody využití MeSH
The prtP-prtM intergenic region of Lactobacillus paracasei subsp. paracasei BGHN 14 was cloned and sequenced. The nucleotide sequence of the prtP-prtM intergenic region in BGHN 14, containing divergently orientated P(prtP) and P(prtP) promoters, was shorter by 35 bp in comparison with that in lactococci. The nucleotide sequence involved in casitone-dependent transcriptional regulation of the lactococcal prt genes was not found in the BGHN14. The activity of P(prtM) in L. lactis NZ9000 was very low and insignificantly changed in the presence of casitone, whereas P(prtP) was completely inactive. When L. casei ATCC393(T) was used as host, both P(prtP) and P(prtM) were active and strongly regulated by casitone. The results strongly indicate that the mechanisms of the casitone-dependent regulation of the prt genes in BGHN14 and lactococci are different.
Lit.: 29
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- 20111210122801.0
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- 040 __
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- $a eng
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- 100 1_
- $a Paštar, I.
- 245 10
- $a Casitone-dependent transcriptional regulation of the prtP and prtM genes in the natural isolate Lactobacillus paracasei subsp. paracasei / $c I. Paštar, J. Begović, J. Lozo, L. Topisirović, N. Golić
- 314 __
- $a Institute of Molecular Genetics and Genetic Engineering, Belgrade
- 504 __
- $a Lit.: 29
- 520 9_
- $a The prtP-prtM intergenic region of Lactobacillus paracasei subsp. paracasei BGHN 14 was cloned and sequenced. The nucleotide sequence of the prtP-prtM intergenic region in BGHN 14, containing divergently orientated P(prtP) and P(prtP) promoters, was shorter by 35 bp in comparison with that in lactococci. The nucleotide sequence involved in casitone-dependent transcriptional regulation of the lactococcal prt genes was not found in the BGHN14. The activity of P(prtM) in L. lactis NZ9000 was very low and insignificantly changed in the presence of casitone, whereas P(prtP) was completely inactive. When L. casei ATCC393(T) was used as host, both P(prtP) and P(prtM) were active and strongly regulated by casitone. The results strongly indicate that the mechanisms of the casitone-dependent regulation of the prt genes in BGHN14 and lactococci are different.
- 650 _2
- $a Lactobacillus $x genetika $x růst a vývoj $7 D007778
- 650 _2
- $a regulace genové exprese $x imunologie $x účinky záření $7 D005786
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- 650 _2
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- 650 _2
- $a polymerázová řetězová reakce $x využití $7 D016133
- 650 _2
- $a promotorové oblasti (genetika) $x imunologie $x účinky záření $7 D011401
- 650 _2
- $a financování organizované $x využití $7 D005381
- 700 1_
- $a Begović, J.
- 700 1_
- $a Lozo, J.
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- $a Topisirović, L.
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- $a Golić, N.
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- LZP __
- $a 2008-11/mkal
