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Yeast vitality determination based on intracellular NAD(P)H fluorescence measurement during aerobic-anaerobic transition
Kuřec M, Gabriela Kuncová, Brányik T
Folia microbiologica.
2009 ;
54 (1) :
25-29.
Language English Country Czech Republic
Persistent link
https://www.medvik.cz/link/bmc07527879
- MeSH
- Aerobiosis MeSH
- Anaerobiosis MeSH
- Financing, Organized MeSH
- Spectrometry, Fluorescence methods MeSH
- Microbial Viability MeSH
- NAD metabolism MeSH
- NADP metabolism MeSH
- Saccharomyces cerevisiae metabolism growth & development MeSH
This work presents a yeast-cell vitality-assessment method based on on-line intracellular fluorescence measurement. The intracellular NAD(P)H fluorescence of a cell suspension is recorded during transition from aerobic to anaerobic conditions and the output signal is evaluated as a measure of yeast vitality (quality). This fluorescence method showed a highly satisfactory correlation with even low dead cell numbers where the acidification power test could not be applied.
Bibliography, etc.
Lit.: 20
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- $a Department of Fermentation Chemistry and Bioengineering, Institute of Chemical Technology, Prague
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- $a Lit.: 20
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- $a This work presents a yeast-cell vitality-assessment method based on on-line intracellular fluorescence measurement. The intracellular NAD(P)H fluorescence of a cell suspension is recorded during transition from aerobic to anaerobic conditions and the output signal is evaluated as a measure of yeast vitality (quality). This fluorescence method showed a highly satisfactory correlation with even low dead cell numbers where the acidification power test could not be applied.
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