• Something wrong with this record ?

Evaluation of the V404I and V509M amino acid substitutions of ERG11 gene in Candida albicans isolates by pyrosequencing

Kim TH, Lee MK.

. 2010 ; 55 (3) : 301-304.

Language English Country Czech Republic

Persistent link   https://www.medvik.cz/link/bmc10017844

The molecular mechanisms underlying fluconazole resistance in C. albicans involve mutations and the overexpression of the ERG11 gene and membrane transport proteins. We examined the relationship between the reduced fluconazole susceptibility of C. albicans and mutations of V404I and V509M in the ERG11 gene in 182 C. albicans clinical isolates using the Pyrosequencing method. DNAs from these clinical isolates with different levels of in-vitro fluconazole susceptibility--one resistant, five susceptible dose-dependent (SDD), four trailer, and 172 susceptible--were analyzed. None of the fluconazole-susceptible, SDD, trailer or resistant isolates had mutations of V404I or V509M. Our results showed that no correlation can be found between the V404I or V509M mutation and fluconazole susceptibility in C. albicans.

Bibliography, etc.

Lit.: 21

000      
00000naa 2200000 a 4500
001      
bmc10017844
003      
CZ-PrNML
005      
20111210182622.0
008      
101125s2010 xr e eng||
009      
AR
024    7_
$2 doi $a 10.1007/s12223-010-0045-2
040    __
$a ABA008 $b cze $c ABA008 $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xr
100    1_
$a Kim, T. H.
245    10
$a Evaluation of the V404I and V509M amino acid substitutions of ERG11 gene in Candida albicans isolates by pyrosequencing / $c Kim TH, Lee MK.
314    __
$a Department of Urology, Chung-Ang University College of Medicine, Seoul
504    __
$a Lit.: 21
520    9_
$a The molecular mechanisms underlying fluconazole resistance in C. albicans involve mutations and the overexpression of the ERG11 gene and membrane transport proteins. We examined the relationship between the reduced fluconazole susceptibility of C. albicans and mutations of V404I and V509M in the ERG11 gene in 182 C. albicans clinical isolates using the Pyrosequencing method. DNAs from these clinical isolates with different levels of in-vitro fluconazole susceptibility--one resistant, five susceptible dose-dependent (SDD), four trailer, and 172 susceptible--were analyzed. None of the fluconazole-susceptible, SDD, trailer or resistant isolates had mutations of V404I or V509M. Our results showed that no correlation can be found between the V404I or V509M mutation and fluconazole susceptibility in C. albicans.
650    _2
$a financování organizované $7 D005381
650    _2
$a substituce aminokyselin $x genetika $7 D019943
650    _2
$a antifungální látky $x farmakologie $7 D000935
650    _2
$a sekvence nukleotidů $7 D001483
650    _2
$a Candida albicans $x enzymologie $x genetika $x účinky léků $7 D002176
650    _2
$a systém (enzymů) cytochromů P-450 $x genetika $x metabolismus $7 D003577
650    _2
$a DNA fungální $x genetika $x chemie $7 D004271
650    _2
$a fungální léková rezistence $7 D025141
650    _2
$a flukonazol $x farmakologie $7 D015725
650    _2
$a fungální proteiny $x genetika $x metabolismus $7 D005656
650    _2
$a lidé $7 D006801
650    _2
$a molekulární sekvence - údaje $7 D008969
650    _2
$a sekvenční analýza DNA $7 D017422
700    1_
$a Lee, M. K.
773    0_
$w MED00011005 $t Folia microbiologica $g Roč. 55, č. 3 (2010), s. 301-304 $x 0015-5632
910    __
$a ABA008 $b A 2403 $c 561 $y 7
990    __
$a 20100825081637 $b ABA008
991    __
$a 20101125112011 $b ABA008
999    __
$a ok $b bmc $g 766871 $s 630764
BAS    __
$a 3
BMC    __
$a 2010 $b 55 $c 3 $d 301-304 $m Folia microbiologica $x MED00011005
LZP    __
$a 2010-22/mkme

Find record