-
Je něco špatně v tomto záznamu ?
Microdispersed Oxidized Cellulose as a novel potential substance with hypolipidemic properties
G. Jamborová, N. Pospíšilová, V. Semecký, R Hyšpler, A. Tichá, K. Pospěchová, D. Solichová, M. Maxová, J. Briestenský, K.J. Real, P. Nachtigal
Jazyk angličtina Země Spojené státy americké
Typ dokumentu práce podpořená grantem
NLK
ProQuest Central
od 2003-01-01 do Před 2 měsíci
Nursing & Allied Health Database (ProQuest)
od 2003-01-01 do Před 2 měsíci
Health & Medicine (ProQuest)
od 2003-01-01 do Před 2 měsíci
Health Management Database (ProQuest)
od 2003-01-01 do Před 2 měsíci
Public Health Database (ProQuest)
od 2003-01-01 do Před 2 měsíci
ScienceDirect (archiv)
od 1996-01-01 do 2009-12-31
- MeSH
- apolipoproteiny E genetika nedostatek MeSH
- celulosa oxidovaná farmakologie MeSH
- cholesterol krev MeSH
- fermentace MeSH
- HDL-cholesterol krev MeSH
- hypolipidemika farmakologie MeSH
- intestinální absorpce fyziologie účinky léků MeSH
- kyseliny mastné těkavé analýza MeSH
- modely nemocí na zvířatech MeSH
- myši inbrední C57BL MeSH
- myši knockoutované MeSH
- myši MeSH
- náhodné rozdělení MeSH
- potravní vláknina aplikace a dávkování farmakologie MeSH
- receptory LDL genetika nedostatek MeSH
- tlusté střevo metabolismus MeSH
- VLDL-cholesterol krev MeSH
- výsledek terapie MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- práce podpořená grantem MeSH
OBJECTIVE: The aim of this study was to determine whether Microdispersed Oxidized Cellulose (MDOC) possesses a hypolipidemic effect in apolipoprotein-E/low-density lipoprotein receptor double-knockout (ApoE/LDLR-deficient) mice and the possible mechanism of this effect in mice. METHODS: Female ApoE/LDLR-deficient mice subdivided into two groups were fed with a Western-type diet for 8 wk, and the experimental group was supplemented with 5% MDOC for 8 wk. Female C57BL/6J mice were fed an atherogenic diet containing 5% MDOC or pectin for the determination of a possible hypolipidemic mechanism of MDOC action. RESULTS: Biochemical analysis showed that 5% MDOC treatment significantly decreased total cholesterol by 20% (P = 0.0338) and very-LDL cholesterol by 21% (P = 0.0110) and significantly increased the level of high-density lipoprotein cholesterol by 62% (P = 0.0172) when compared with non-treated ApoE/LDLR-deficient mice. The results Association of Official Analytical Chemists method 991.43 revealed that MDOC contains 59.78 +/- 5.0% of fiber. Furthermore, it was demonstrated that administration of MDOC did not affect cholesterol absorption in the small intestine. Using C57BL/6J mice, MDOC and pectin treatments decreased cholesterol content in liver and increased fermentation in the gut in vivo. In vitro experiments confirmed that MDOC is fermentable under conditions mimicking those in the large intestine. CONCLUSION: We demonstrated hypolipidemic effects of MDOC in ApoE/LDLR-deficient mice. Moreover, we propose that MDOC is a hypolipidemic soluble fiber acting probably by increased fermentation and production of short-chain fatty acids in the large intestine in mice. We propose that MDOC might be a possible source of soluble fiber for use in dietary supplements.
- 000
- 02580naa 2200613 a 4500
- 001
- bmc11006335
- 003
- CZ-PrNML
- 005
- 20121113124042.0
- 008
- 110401s2008 xxu e eng||
- 009
- AR
- 040 __
- $a ABA008 $b cze $c ABA008 $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Jamborová, Gabriela. $7 _BN003298
- 245 10
- $a Microdispersed Oxidized Cellulose as a novel potential substance with hypolipidemic properties / $c G. Jamborová, N. Pospíšilová, V. Semecký, R Hyšpler, A. Tichá, K. Pospěchová, D. Solichová, M. Maxová, J. Briestenský, K.J. Real, P. Nachtigal
- 314 __
- $a Department of Biological and Medical Sciences, Faculty of Pharmacy, Charles University, Hradec Kralove, Czech Republic.
- 520 9_
- $a OBJECTIVE: The aim of this study was to determine whether Microdispersed Oxidized Cellulose (MDOC) possesses a hypolipidemic effect in apolipoprotein-E/low-density lipoprotein receptor double-knockout (ApoE/LDLR-deficient) mice and the possible mechanism of this effect in mice. METHODS: Female ApoE/LDLR-deficient mice subdivided into two groups were fed with a Western-type diet for 8 wk, and the experimental group was supplemented with 5% MDOC for 8 wk. Female C57BL/6J mice were fed an atherogenic diet containing 5% MDOC or pectin for the determination of a possible hypolipidemic mechanism of MDOC action. RESULTS: Biochemical analysis showed that 5% MDOC treatment significantly decreased total cholesterol by 20% (P = 0.0338) and very-LDL cholesterol by 21% (P = 0.0110) and significantly increased the level of high-density lipoprotein cholesterol by 62% (P = 0.0172) when compared with non-treated ApoE/LDLR-deficient mice. The results Association of Official Analytical Chemists method 991.43 revealed that MDOC contains 59.78 +/- 5.0% of fiber. Furthermore, it was demonstrated that administration of MDOC did not affect cholesterol absorption in the small intestine. Using C57BL/6J mice, MDOC and pectin treatments decreased cholesterol content in liver and increased fermentation in the gut in vivo. In vitro experiments confirmed that MDOC is fermentable under conditions mimicking those in the large intestine. CONCLUSION: We demonstrated hypolipidemic effects of MDOC in ApoE/LDLR-deficient mice. Moreover, we propose that MDOC is a hypolipidemic soluble fiber acting probably by increased fermentation and production of short-chain fatty acids in the large intestine in mice. We propose that MDOC might be a possible source of soluble fiber for use in dietary supplements.
- 590 __
- $a bohemika - dle Pubmed
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a apolipoproteiny E $x genetika $x nedostatek $7 D001057
- 650 _2
- $a celulosa oxidovaná $x farmakologie $7 D002483
- 650 _2
- $a cholesterol $x krev $7 D002784
- 650 _2
- $a HDL-cholesterol $x krev $7 D008076
- 650 _2
- $a VLDL-cholesterol $x krev $7 D015243
- 650 _2
- $a potravní vláknina $x aplikace a dávkování $x farmakologie $7 D004043
- 650 _2
- $a modely nemocí na zvířatech $7 D004195
- 650 _2
- $a kyseliny mastné těkavé $x analýza $7 D005232
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a fermentace $7 D005285
- 650 _2
- $a hypolipidemika $x farmakologie $7 D000960
- 650 _2
- $a intestinální absorpce $x fyziologie $x účinky léků $7 D007408
- 650 _2
- $a tlusté střevo $x metabolismus $7 D007420
- 650 _2
- $a myši $7 D051379
- 650 _2
- $a myši inbrední C57BL $7 D008810
- 650 _2
- $a myši knockoutované $7 D018345
- 650 _2
- $a náhodné rozdělení $7 D011897
- 650 _2
- $a receptory LDL $x genetika $x nedostatek $7 D011973
- 650 _2
- $a výsledek terapie $7 D016896
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Pospíšilová, Naďa $7 xx0095413
- 700 1_
- $a Semecký, Vladimír, $d 1943- $7 nlk19990073843
- 700 1_
- $a Hyšpler, Radomír, $d 1972- $7 nlk20040155091
- 700 1_
- $a Tichá, Alena, $d 1976- $7 xx0076494
- 700 1_
- $a Pospěchová, Kateřina $7 xx0104147
- 700 1_
- $a Solichová, Dagmar $7 xx0060526
- 700 1#
- $a Maxová, Martina. $7 _BN003292
- 700 1_
- $a Briestenský, Jiří $7 xx0103976
- 700 1_
- $a Real, Keith J
- 700 1_
- $a Nachtigal, Petr $7 uk2009304471
- 773 0_
- $t Nutrition $g Roč. 24, č. 11-12 (2008), s. 1174-1181 $x 0899-9007 $w MED00003566
- 910 __
- $a ABA008 $b x $y 2
- 990 __
- $a 20110414103643 $b ABA008
- 991 __
- $a 20121113124057 $b ABA008
- 999 __
- $a ok $b bmc $g 833937 $s 698432
- BAS __
- $a 3
- BMC __
- $a 2008 $b 24 $c 11-12 $d 1174-1181 $i 0899-9007 $m Nutrition $n Nutrition $x MED00003566
- LZP __
- $a 2011-1B09/jjme