The antimicrobial effect of OKCEL® H-D, a topical, absorbable hemostatic textile prepared from oxidized cellulose, was tested. Testing by dilution and diffusion methods was conducted on a spectrum of 27 select microorganisms, including also antibiotic-resistant strains. OKCEL® H-D showed inhibitory effects on nearly all tested bacteria. In testing using the dilution suspension method, the majority of bacteria showed decrease in cell density by 7-8 orders of magnitude after just 6 h of exposure. For clinical isolates of antibiotic-resistant strains, a reduction occurred after 24 h of exposure. In testing the antimicrobial effects of OKCEL® H-D by the dilution method was least effective on spore-forming Bacillus subtilis, for which no antimicrobial effect was detected after 48 h, and on Mycobacterium smegmatis, for which the number of cells decreased by four orders of magnitude only after 24 h. By the diffusion method, inhibition zones were recorded for nearly all test microorganisms except for Staphylococcus aureus, M. smegmatis, and Listeria monocytogenes. No growth beneath the tested OKCEL® H-D material was recorded, however, even for the latter-named bacteria strains, which attests to its good inhibitory effect.
- MeSH
- antibakteriální látky chemie farmakologie MeSH
- Bacillus subtilis účinky léků růst a vývoj MeSH
- bakteriální léková rezistence MeSH
- celulosa oxidovaná chemie farmakologie MeSH
- Listeria monocytogenes účinky léků růst a vývoj MeSH
- mikrobiální testy citlivosti MeSH
- Staphylococcus aureus účinky léků růst a vývoj MeSH
- Publikační typ
- časopisecké články MeSH
- Klíčová slova
- Traumacel,
- MeSH
- celulosa oxidovaná * farmakologie terapeutické užití MeSH
- hemostatika chemie terapeutické užití MeSH
- krvácení při operaci prevence a kontrola MeSH
- lidé MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- novinové články MeSH
Novel hydrolytically stable gelatin nanofibers modified with sodium or calcium salt of oxycellulose were prepared by electrospinning method. The unique inhibitory effect of these nanofibers against Escherichia coli bacteria was examined by luminometric method. Biocompatibility of these gelatin/oxycellulose nanofibers with eukaryotic cells was tested using human lung adenocarcinoma cell line NCI-H441. Cells firmly adhered to nanofiber surface, as determined by scanning electron microscopy, and no signs of cell dying were detected by fluorescent live/dead assay. We propose that the newly developed gelatin/oxycellulose nanofibers could be used as promising scaffold for lung disease modeling and anti-cancer drug testing.
- MeSH
- adenokarcinom farmakoterapie metabolismus patologie MeSH
- celulosa oxidovaná * chemie farmakologie MeSH
- Escherichia coli růst a vývoj MeSH
- lidé MeSH
- nádorové buněčné linie MeSH
- nádory plic farmakoterapie metabolismus patologie MeSH
- nanovlákna chemie MeSH
- želatina * chemie farmakologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Celulózové materiály nebo jejich deriváty nabízejí pro hojení ran řadu cenných vlastností. Karboxylované celulózy zastupuje pře-devším karboxymethylovaná celulóza a oxidovaná celulóza. Hcel HT (karboxymethylovaná celulóza) je nový typ celulózového krytí s kyselým povrchovým pH.
Levels of matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9) and tumor necrosis factor-α (TNF-α) may influence wound healing and wound closure in non-healing wounds. The aim of the study was to test the hypothesis that hydrogencalcium salts of oxidized cellulose change the production of matrix metalloproteinases (MMPs) and TNF-α, wound size and number of bacterial strains in non-healing wounds. We analyzed MMP-2, MMP-9 and TNF-α in the wound fluid from 20 patients by ELISA every fourteen days over six weeks. Wound size, pain, wound closure and bacterial strains in the wound were also investigated. The wound size was reduced in 14 patients and pain in 16 patients. Bacterial contamination of the wound decreased significantly after treatment. The level of MMP-2 correlated with TNF-α production. The level of MMP-9 was unchanged during the healing period. We conclude that hydrogencalcium salts of oxidized cellulose have a favorable effect on the reduction of bacterial contamination, wound size and pain.
- MeSH
- bércové vředy metabolismus mikrobiologie terapie MeSH
- celulosa oxidovaná farmakologie MeSH
- diabetická noha metabolismus mikrobiologie terapie MeSH
- hojení ran fyziologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- matrixová metaloproteinasa 2 metabolismus MeSH
- matrixová metaloproteinasa 9 metabolismus MeSH
- senioři MeSH
- TNF-alfa metabolismus MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The aim of this study was to examine the effect of topically-used gentamicin attached to a biodegradable carrier, formed by micro-dispersed oxidised cellulose (MDOC) in nanofibre form, in acute wound infection treatment and to compare it with Garamycin Schwamm®. Twelve domestic swines were used in a model of a full-thickness infected dermal wound. The effectiveness of both materials in wound infections caused by Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli was tested. The effectiveness of both gentamicin with MDOC and Garamycin Schwamm® was comparable in Pseudomonas aeruginosa and Escherichia coli infections according to microbiological cultures findings. With Staphylococcus aureus infections there was a higher percent of negative cultures when MDOC with gentamicin was administered, but without statistical significance (p=0.069). When macroscopically assesed, 100 % of infected wounds treated by gentamicin attached to MDOC were without signs of local infection compared to only 16.7 % when Garamycin Schwamm® was used and this was statistically significant (p<0.01). For statistical analysis we used a Fisher´s exact test. When combined with a nanofibre MDOC carrier, topically-used gentamicin seems to be rendered more for treatment of full-thickness skin infections. The resulting good haemostatic effect of MDOC was observed.
- MeSH
- aplikace lokální MeSH
- celulosa oxidovaná farmakologie terapeutické užití MeSH
- Escherichia coli účinky léků MeSH
- financování organizované MeSH
- gentamiciny farmakologie terapeutické užití MeSH
- hodnocení léčiv statistika a číselné údaje MeSH
- hojení ran účinky léků MeSH
- infekce chirurgické rány farmakoterapie mikrobiologie MeSH
- infekční nemoci kůže farmakoterapie mikrobiologie MeSH
- kultivační techniky MeSH
- modely nemocí na zvířatech MeSH
- prasata MeSH
- Pseudomonas aeruginosa účinky léků MeSH
- Staphylococcus aureus účinky léků MeSH
- statistika jako téma MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- srovnávací studie MeSH
The aim of this experimental in vivo study was to examine the effect of topically-used gentamicin bound to microdispersed oxidized cellulose (MDOC) in nanofibre form in the treatment of deep surgical site infections and to compare it with Garamycin Schwamm®. Twelve domestic swine were used in a model of a full-thickness infected dermal wound. The effectiveness of both forms of gentamicin were tested in wound infections caused by Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli. The effectiveness of gentamicin-MDOC and Garamycin Schwamm® were comparable according to microbiological culture findings, with no statistically significant differences. When macroscopically assessed, 100% of the infected wounds treated by gentamicin-MDOC were without signs of infection, compared with only 16.7% when Garamycin Schwamm® was used and this was of statistical significance. Therefore when combined with a nanofibre MDOC carrier, topically-used gentamicin is rendered more effective for the treatment of full-thickness skin infections.
- MeSH
- antibakteriální látky aplikace a dávkování terapeutické užití MeSH
- aplikace lokální MeSH
- Bacteria izolace a purifikace MeSH
- celulosa oxidovaná farmakologie terapeutické užití MeSH
- financování organizované MeSH
- gentamiciny terapeutické užití MeSH
- hojení ran účinky léků MeSH
- infekce chirurgické rány farmakoterapie MeSH
- infekce v ráně farmakoterapie mikrobiologie MeSH
- infekce vyvolané Escherichia coli chemicky indukované MeSH
- kolagen terapeutické užití MeSH
- nosiče léků MeSH
- prasata MeSH
- příprava léků metody MeSH
- Pseudomonas aeruginosa MeSH
- Staphylococcus aureus MeSH
- výsledek terapie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- srovnávací studie MeSH
- Klíčová slova
- Surgicel,
- MeSH
- celulosa oxidovaná aplikace a dávkování farmakologie terapeutické užití MeSH
- hemostatické techniky MeSH
- hemostatika terapeutické užití MeSH
- hemostáza chirurgická ošetřování MeSH
- hemostáza účinky léků MeSH
- krvácení při operaci prevence a kontrola MeSH
- lidé MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- novinové články MeSH
- MeSH
- biokompatibilní materiály farmakologie terapeutické užití MeSH
- celulosa oxidovaná farmakologie terapeutické užití MeSH
- hojení ran fyziologie účinky léků MeSH
- jizva hypertrofická MeSH
- lidé MeSH
- obvazy hydrokoloidní využití MeSH
- senioři MeSH
- Check Tag
- lidé MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
