The aim of this experimental in vivo study was to examine the effect of topically-used gentamicin bound to microdispersed oxidized cellulose (MDOC) in nanofibre form in the treatment of deep surgical site infections and to compare it with Garamycin Schwamm®. Twelve domestic swine were used in a model of a full-thickness infected dermal wound. The effectiveness of both forms of gentamicin were tested in wound infections caused by Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli. The effectiveness of gentamicin-MDOC and Garamycin Schwamm® were comparable according to microbiological culture findings, with no statistically significant differences. When macroscopically assessed, 100% of the infected wounds treated by gentamicin-MDOC were without signs of infection, compared with only 16.7% when Garamycin Schwamm® was used and this was of statistical significance. Therefore when combined with a nanofibre MDOC carrier, topically-used gentamicin is rendered more effective for the treatment of full-thickness skin infections.

• MeSH
• antibakteriální látky aplikace a dávkování   terapeutické užití   MeSH
• aplikace lokální MeSH
• Bacteria izolace a purifikace   MeSH
• celulosa oxidovaná farmakologie   terapeutické užití   MeSH
• financování organizované MeSH
• gentamiciny terapeutické užití   MeSH
• hojení ran účinky léků   MeSH
• infekce chirurgické rány farmakoterapie   MeSH
• infekce v ráně farmakoterapie   mikrobiologie   MeSH
• infekce vyvolané Escherichia coli chemicky indukované   MeSH
• kolagen terapeutické užití   MeSH
• nosiče léků MeSH
• prasata MeSH
• příprava léků metody   MeSH
• Pseudomonas aeruginosa MeSH
• Staphylococcus aureus MeSH
• výsledek terapie MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• srovnávací studie MeSH

The purpose of our study was to evaluate changes of the cerebral microcirculation during the early stages of endotoxemia in mechanically-ventilated rabbits using Sidestream dark-field (SDF) imaging. Images were obtained using SDF imaging from the surface of the brain via craniotomy before and after rapid administration of a high dose of endotoxin or saline (control group). Although endotoxin shock was successfully induced, we have not found any significant alteration of the cerebral microcirculation during the shock. We speculate that either the model of sepsis with a rapid high dose of endotoxin does not reflect the usual progression of septic encephalopathy or some components other than cerebral microcirculatory alteration play a role at the early stage of septic encephalopathy and the cerebral microcirculation is still preserved. Further studies are needed to clarify our findings.

• MeSH
• endotoxemie chemicky indukované   patologie   patofyziologie   terapie   MeSH
• endotoxiny MeSH
• infekce vyvolané Escherichia coli chemicky indukované   patologie   patofyziologie   terapie   MeSH
• králíci MeSH
• mikrocirkulace MeSH
• mozek krevní zásobení   patologie   patofyziologie   MeSH
• septický šok MeSH
• umělé dýchání MeSH
• zvířata MeSH
• Check Tag
• králíci MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH