• Je něco špatně v tomto záznamu ?

Mycobacterium avium subsp. paratuberculosis in cow bulk tank milk in Cyprus detected by culture and quantitative IS900 and F57 real-time PCR

I. Slaná, M. Liapi, M. Morávková, A. Králová, I. Pavlík

. 2009 ; 89 (3-4) : 223-226.

Jazyk angličtina Země Nizozemsko

Typ dokumentu srovnávací studie, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc11017214

The possibility that Mycobacterium avium subsp. paratuberculosis (MAP) plays some role in the development of Crohn's disease in humans is attracting attention to milk and milk products originating from infected animals. In this study, we focused on the detection of MAP in 220 bulk tank milk (BTM) samples from all dairy cattle herds in Cyprus. In total, 63 (28.6%) BTM milk samples were found to be positive for MAP using quantitative real-time PCR assays for IS900 and F57. The presence of MAP in BTM was low, and was assessed to be several tens of MAP cells per one ml of BTM. Milk samples examined by cultivation were found to be negative for MAP in all 220 BTM. In two BTM samples cultivation and subsequent sequencing of 16S rRNA revealed two isolates of M. fortuitum.

000      
02662naa 2200493 a 4500
001      
bmc11017214
003      
CZ-PrNML
005      
20150818112718.0
008      
110629s2009 ne e eng||
009      
AR
040    __
$a ABA008 $b cze $c ABA008 $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a ne
100    1_
$a Slaná, Iva $7 uzp2009545460
245    10
$a Mycobacterium avium subsp. paratuberculosis in cow bulk tank milk in Cyprus detected by culture and quantitative IS900 and F57 real-time PCR / $c I. Slaná, M. Liapi, M. Morávková, A. Králová, I. Pavlík
314    __
$a Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic.
520    9_
$a The possibility that Mycobacterium avium subsp. paratuberculosis (MAP) plays some role in the development of Crohn's disease in humans is attracting attention to milk and milk products originating from infected animals. In this study, we focused on the detection of MAP in 220 bulk tank milk (BTM) samples from all dairy cattle herds in Cyprus. In total, 63 (28.6%) BTM milk samples were found to be positive for MAP using quantitative real-time PCR assays for IS900 and F57. The presence of MAP in BTM was low, and was assessed to be several tens of MAP cells per one ml of BTM. Milk samples examined by cultivation were found to be negative for MAP in all 220 BTM. In two BTM samples cultivation and subsequent sequencing of 16S rRNA revealed two isolates of M. fortuitum.
590    __
$a bohemika - dle Pubmed
650    _2
$a zvířata $7 D000818
650    _2
$a skot $7 D002417
650    _2
$a nemoci skotu $x diagnóza $x přenos $7 D002418
650    _2
$a počet mikrobiálních kolonií $x veterinární $7 D015169
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a kontaminace potravin $x analýza $7 D005506
650    _2
$a lidé $7 D006801
650    _2
$a plošný screening $x veterinární $7 D008403
650    _2
$a mléko $x mikrobiologie $7 D008892
650    _2
$a Mycobacterium avium subsp. paratuberculosis $x izolace a purifikace $7 D016927
650    _2
$a paratuberkulóza $x diagnóza $x přenos $7 D010283
650    _2
$a polymerázová řetězová reakce $x veterinární $7 D016133
650    _2
$a prediktivní hodnota testů $7 D011237
650    _2
$a bakteriální RNA $x analýza $7 D012329
650    _2
$a RNA ribozomální 16S $x analýza $7 D012336
650    _2
$a senzitivita a specificita $7 D012680
650    _2
$a zoonózy $7 D015047
651    _2
$a Česká republika $7 D018153
655    _2
$a srovnávací studie $7 D003160
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Liapi, M.
700    1_
$a Morávková, Monika $7 xx0098544
700    1_
$a Králová, Alena $7 xx0122374
700    1_
$a Pavlík, Ivo, $d 1961- $7 xx0037127
773    0_
$t Preventive Veterinary Medicine $w MED00006663 $g Roč. 89, č. 3-4 (2009), s. 223-226 $x 0167-5877
910    __
$a ABA008 $b x $y 2 $z 0
990    __
$a 20110720103219 $b ABA008
991    __
$a 20150818112826 $b ABA008
999    __
$a ok $b bmc $g 864199 $s 727012
BAS    __
$a 3
BMC    __
$a 2009 $x MED00006663 $b 89 $c 3-4 $d 223-226 $m Preventive veterinary medicine $n Prev Vet Med $i 0167-5877
LZP    __
$a 2011-3B09/BBjvme

Najít záznam

Citační ukazatele

Možnosti archivace