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Mycobacterium avium subsp. paratuberculosis in cow bulk tank milk in Cyprus detected by culture and quantitative IS900 and F57 real-time PCR
I. Slaná, M. Liapi, M. Morávková, A. Králová, I. Pavlík
Language English Country Netherlands
Document type Comparative Study, Research Support, Non-U.S. Gov't
- MeSH
- RNA, Bacterial analysis MeSH
- Food Contamination analysis MeSH
- Humans MeSH
- Milk microbiology MeSH
- Mycobacterium avium subsp. paratuberculosis isolation & purification MeSH
- Cattle Diseases diagnosis transmission MeSH
- Paratuberculosis diagnosis transmission MeSH
- Mass Screening veterinary MeSH
- Colony Count, Microbial veterinary MeSH
- Polymerase Chain Reaction veterinary MeSH
- Predictive Value of Tests MeSH
- RNA, Ribosomal, 16S analysis MeSH
- Sensitivity and Specificity MeSH
- Cattle MeSH
- Zoonoses MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Cattle MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Geographicals
- Czech Republic MeSH
The possibility that Mycobacterium avium subsp. paratuberculosis (MAP) plays some role in the development of Crohn's disease in humans is attracting attention to milk and milk products originating from infected animals. In this study, we focused on the detection of MAP in 220 bulk tank milk (BTM) samples from all dairy cattle herds in Cyprus. In total, 63 (28.6%) BTM milk samples were found to be positive for MAP using quantitative real-time PCR assays for IS900 and F57. The presence of MAP in BTM was low, and was assessed to be several tens of MAP cells per one ml of BTM. Milk samples examined by cultivation were found to be negative for MAP in all 220 BTM. In two BTM samples cultivation and subsequent sequencing of 16S rRNA revealed two isolates of M. fortuitum.
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