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Synthesis of LacdiNAc-terminated glycoconjugates by mutant galactosyltransferase - a way to new glycodrugs and materials
P. Bojarová, K. Křenek, K. Wetjen, K. Adamiak, H. Pelantová, K. Bezouška, L. Elling, V. Křen
Status retracted Language English Country Great Britain
Document type Research Support, Non-U.S. Gov't, Retracted Publication
NLK
Free Medical Journals
from 1996 to 1 year ago
Open Access Digital Library
from 1996-01-01
Medline Complete (EBSCOhost)
from 1996-01-01 to 1 year ago
PubMed
19179461
DOI
10.1093/glycob/cwp010
Knihovny.cz E-resources
- MeSH
- Bacterial Proteins metabolism MeSH
- Campylobacter jejuni enzymology MeSH
- Antigens, CD metabolism MeSH
- Antigens, Differentiation, T-Lymphocyte metabolism MeSH
- Carbohydrate Epimerases metabolism MeSH
- Galactosyltransferases genetics metabolism MeSH
- Glycoconjugates biosynthesis chemical synthesis metabolism MeSH
- Lactose analogs & derivatives biosynthesis chemical synthesis metabolism MeSH
- NK Cell Lectin-Like Receptor Subfamily B metabolism MeSH
- Lectins, C-Type MeSH
- Humans MeSH
- Mutation MeSH
- Placenta enzymology MeSH
- Substrate Specificity MeSH
- Pregnancy MeSH
- Check Tag
- Humans MeSH
- Pregnancy MeSH
- Female MeSH
- Publication type
- Retracted Publication MeSH
- Research Support, Non-U.S. Gov't MeSH
Human placental beta1,4-galactosyltransferase-I (EC 2.4.1.38) transfers the galactosyl moiety from UDP-Gal to various GlcNAc or Glc acceptors in vivo. Here, we describe the construction of its Y284L mutant as a His(6)propeptide-catbeta4GalT1 construct, in which the Gal-transferase activity was totally abolished in favor of its GalNAc-transferase activity. We used this mutant in the synthesis of three mono- and bivalent LacdiNAc glycomimetics with good yields. These compounds proved to be powerful ligands of two activation receptors of natural killer cells, NKR-P1 and CD69. A synthetic bivalent tethered di-LacdiNAc is the best currently known precipitation agent for both of these receptors and has promising potential for the development of immunoactive glycodrugs.
References provided by Crossref.org
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