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Comparing electron microscopy and a competitive blocking ELISA in the detection of rotaviruses in porcine faeces
L. Rodák, R. Smítalová, B. Šmíd, Z. Nevoránková, L. Valíček, I. Pšikal
Language English Country England, Great Britain
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Microscopy, Electron methods veterinary MeSH
- Enzyme-Linked Immunosorbent Assay methods veterinary MeSH
- Feces virology MeSH
- Swine Diseases diagnosis virology MeSH
- Swine MeSH
- Diarrhea veterinary virology MeSH
- Rotavirus Infections diagnosis veterinary virology MeSH
- Rotavirus isolation & purification MeSH
- Sensitivity and Specificity MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
Analysis of 476 faecal samples from diarrhoeic piglets was performed using electron microscopy (EM) and a competitive blocking (CB)-ELISA based on monoclonal antibodies to the VP6 protein of group A rotavirus. Rotavirus was detected by EM and/or CB-ELISA in 111 (23.3%) samples. Of these, all groups of rotavirus were identified in 83 (74.8%) samples by EM (EM+), while group A rotavirus was identified in 90 (81.1%) samples by CB-ELISA (ELISA+). However, only 62 (55.9%) of samples were positive using both detection methods. The finding of 28 (25.2%) EM-/CB-ELISA+ samples illustrated the high sensitivity of the CB-ELISA method. On PCR analysis, groups B and C rotavirus was found in 3 and 16 of 19 EM+/CB-ELISA- samples, respectively. Although the study illustrates the high sensitivity of a CB-ELISA in rotavirus detection, the findings highlight the need to use a range of diagnostic methods in detecting these viruses in clinical samples.
References provided by Crossref.org
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