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Gold nanoparticle-based immuno-PCR for detection of tau protein in cerebrospinal fluid
L. Stegurová, E. Dráberová, A. Bartos, P. Dráber, D. Rípová, P. Dráber,
Jazyk angličtina Země Nizozemsko
Typ dokumentu časopisecké články, práce podpořená grantem
Grantová podpora
NT13183
MZ0
CEP - Centrální evidence projektů
- MeSH
- Alzheimerova nemoc diagnóza MeSH
- biologické markery MeSH
- ELISA * MeSH
- kovové nanočástice * MeSH
- lidé MeSH
- monoklonální protilátky imunologie MeSH
- polymerázová řetězová reakce metody MeSH
- proteiny tau MeSH
- senzitivita a specificita MeSH
- tyramin metabolismus MeSH
- zlato * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Tau protein in cerebrospinal fluid (CSF) is an important biomarker of Alzheimer's disease and some other brain diseases. Enzyme-linked immunosorbent assays (ELISAs) have been mostly used for quantification of tau and other biomarkers in CSF. However, these assays do not have sufficient sensitivity and dynamic range. In this study we tested the suitability of gold nanoparticles functionalized with tau-specific monoclonal antibody and oligonucleotide template for immuno-polymerase chain reaction (Nano-iPCR) quantification of tau protein in human CSF samples and compared it with ELISA, either commercial or newly developed with tyramide signal amplification. Our data indicate that Nano-iPCR is superior in sensitivity and detection range to ELISA in tau protein detection.
Citace poskytuje Crossref.org
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