-
Something wrong with this record ?
Total globozoospermia associated with increased frequency of immature spermatozoa with chromatin defects and aneuploidy: a case report
M. Vozdova, R. Rybar, S. Kloudova, P. Prinosilova, P. Texl, J. Rubes,
Language English Country Germany
Document type Case Reports, Journal Article, Research Support, Non-U.S. Gov't
PubMed
24007278
DOI
10.1111/and.12156
Knihovny.cz E-resources
- MeSH
- Semen Analysis methods MeSH
- Aneuploidy * MeSH
- Adult MeSH
- In Situ Hybridization, Fluorescence MeSH
- Middle Aged MeSH
- Humans MeSH
- Infertility, Male genetics MeSH
- DNA Breaks MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Case Reports MeSH
- Research Support, Non-U.S. Gov't MeSH
Globozoospermia, characterised by the presence of round spermatozoa lacking acrosomes in an ejaculate, is a known cause of male infertility. Semen analysis, including sperm chromatin structure assay, toluidine blue, chromomycin A3 and aniline blue staining and fluorescence in situ hybridisation, was performed in an infertile globozoospermic patient to establish to which extent these genetic factors contributed to his infertility. No spermatozoa capable of hyaluronan (HA) binding were detected in the HA binding assay. Increased rates of immature spermatozoa with defective replacement of histones by protamines, DNA breaks and disturbed chromatin integrity and sperm aneuploid for the sex chromosomes were observed. Intracytoplasmic sperm injection (ICSI) was used in three in vitro fertilisation (IVF) cycles, and enough morphologically well-developing embryos were obtained in each cycle. However, no pregnancy was achieved. The infertility of our couple, resistant to IVF/ICSI treatment, was most probably caused by a combination of male and female factors.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc15023633
- 003
- CZ-PrNML
- 005
- 20150722130953.0
- 007
- ta
- 008
- 150709s2014 gw f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1111/and.12156 $2 doi
- 035 __
- $a (PubMed)24007278
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a gw
- 100 1_
- $a Vozdova, M $u Department of Genetics and Reproduction, Veterinary Research Institute, Brno, Czech Republic.
- 245 10
- $a Total globozoospermia associated with increased frequency of immature spermatozoa with chromatin defects and aneuploidy: a case report / $c M. Vozdova, R. Rybar, S. Kloudova, P. Prinosilova, P. Texl, J. Rubes,
- 520 9_
- $a Globozoospermia, characterised by the presence of round spermatozoa lacking acrosomes in an ejaculate, is a known cause of male infertility. Semen analysis, including sperm chromatin structure assay, toluidine blue, chromomycin A3 and aniline blue staining and fluorescence in situ hybridisation, was performed in an infertile globozoospermic patient to establish to which extent these genetic factors contributed to his infertility. No spermatozoa capable of hyaluronan (HA) binding were detected in the HA binding assay. Increased rates of immature spermatozoa with defective replacement of histones by protamines, DNA breaks and disturbed chromatin integrity and sperm aneuploid for the sex chromosomes were observed. Intracytoplasmic sperm injection (ICSI) was used in three in vitro fertilisation (IVF) cycles, and enough morphologically well-developing embryos were obtained in each cycle. However, no pregnancy was achieved. The infertility of our couple, resistant to IVF/ICSI treatment, was most probably caused by a combination of male and female factors.
- 650 _2
- $a dospělí $7 D000328
- 650 12
- $a aneuploidie $7 D000782
- 650 _2
- $a zlomy DNA $7 D053960
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a hybridizace in situ fluorescenční $7 D017404
- 650 _2
- $a mužská infertilita $x genetika $7 D007248
- 650 _2
- $a mužské pohlaví $7 D008297
- 650 _2
- $a lidé středního věku $7 D008875
- 650 _2
- $a analýza spermatu $x metody $7 D055101
- 655 _2
- $a kazuistiky $7 D002363
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Rybar, R
- 700 1_
- $a Kloudova, S
- 700 1_
- $a Prinosilova, P
- 700 1_
- $a Texl, P
- 700 1_
- $a Rubes, J
- 773 0_
- $w MED00000345 $t Andrologia $x 1439-0272 $g Roč. 46, č. 8 (2014), s. 831-6
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/24007278 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20150709 $b ABA008
- 991 __
- $a 20150722131030 $b ABA008
- 999 __
- $a ok $b bmc $g 1083970 $s 906626
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2014 $b 46 $c 8 $d 831-6 $i 1439-0272 $m Andrologia $n Andrologia $x MED00000345
- LZP __
- $a Pubmed-20150709