The aim of this study was to develop the potential tissue engineering applications of d-glucosamine (GlcN) immobilized onto the surface of a biodegradable matrix in order to induce a desired biological effect at biointerfaces. Thus, for sample preparation we used a novel multistep physicochemical approach. In the first step the poly(lactic acid) (PLA) films were exposed to a low pressure plasma in air atmosphere, followed by radical graft copolymerization with acrylic acid to yield a carboxyl-functionalized spacer layer on the PLA surface. The carboxyl groups were then coupled to GlcN molecules via the carbodiimide chemistry. The developed surfaces were characterized by X-ray Photoelectron Spectroscopy (XPS), Contact angle measurements and Atomic Force Microscopy (AFM). A preliminary study on the proliferation of fibroblasts on the developed surfaces was performed using the NIH/3T3 cell line.

Centre of Polymer Systems Tomas Bata University in Zlín Trida Tomase Bati 5678 760 01 Zlín Czech Republic

Department of Chemistry Faculty of Science Ain Shams University Abbassia Cairo 11566 Egypt

Department of Surface Engineering and Optoelectronics Jozef Stefan Institute Jamova Cesta 39 1000 Ljubljana Slovenia

Polymer Institute Slovak Academy of Sciences Dúbravská Cesta 9 845 41 Bratislava Slovakia

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$a Swilem, Ahmed E $u Centre of Polymer Systems, Tomas Bata University in Zlín, Trida Tomase Bati 5678, 760 01 Zlín, Czech Republic; Department of Chemistry, Faculty of Science, Ain Shams University, Abbassia, Cairo 11566, Egypt.

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$a Developing a biomaterial interface based on poly(lactic acid) via plasma-assisted covalent anchorage of d-glucosamine and its potential for tissue regeneration / $c AE. Swilem, M. Lehocký, P. Humpolíček, Z. Kucekova, I. Junkar, M. Mozetič, AA. Hamed, I. Novák,

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$a The aim of this study was to develop the potential tissue engineering applications of d-glucosamine (GlcN) immobilized onto the surface of a biodegradable matrix in order to induce a desired biological effect at biointerfaces. Thus, for sample preparation we used a novel multistep physicochemical approach. In the first step the poly(lactic acid) (PLA) films were exposed to a low pressure plasma in air atmosphere, followed by radical graft copolymerization with acrylic acid to yield a carboxyl-functionalized spacer layer on the PLA surface. The carboxyl groups were then coupled to GlcN molecules via the carbodiimide chemistry. The developed surfaces were characterized by X-ray Photoelectron Spectroscopy (XPS), Contact angle measurements and Atomic Force Microscopy (AFM). A preliminary study on the proliferation of fibroblasts on the developed surfaces was performed using the NIH/3T3 cell line.

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$a Lehocký, Marian $u Centre of Polymer Systems, Tomas Bata University in Zlín, Trida Tomase Bati 5678, 760 01 Zlín, Czech Republic. Electronic address: lehocky@post.cz.

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$a Humpolíček, Petr $u Centre of Polymer Systems, Tomas Bata University in Zlín, Trida Tomase Bati 5678, 760 01 Zlín, Czech Republic.

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$a Kucekova, Zdenka $u Centre of Polymer Systems, Tomas Bata University in Zlín, Trida Tomase Bati 5678, 760 01 Zlín, Czech Republic.

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$a Junkar, Ita $u Department of Surface Engineering and Optoelectronics, Jozef Stefan Institute, Jamova Cesta 39, 1000 Ljubljana, Slovenia.

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$a Mozetič, Miran $u Department of Surface Engineering and Optoelectronics, Jozef Stefan Institute, Jamova Cesta 39, 1000 Ljubljana, Slovenia.

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$a Hamed, Ashraf A $u Department of Chemistry, Faculty of Science, Ain Shams University, Abbassia, Cairo 11566, Egypt.

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$a Novák, Igor $u Polymer Institute, Slovak Academy of Sciences, Dúbravská Cesta 9, 845 41 Bratislava, Slovakia.

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