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Heterogeneity of Human Pancreatic Islet Isolation Around Europe: Results of a Survey Study

R. Nano, JA. Kerr-Conte, H. Scholz, M. Engelse, M. Karlsson, F. Saudek, D. Bosco, B. Antonioli, F. Bertuzzi, PRV. Johnson, B. Ludwing, Z. Ling, DL. De Paep, B. Keymeulen, F. Pattou, T. Berney, O. Korsgren, E. de Koning, L. Piemonti,

. 2020 ; 104 (1) : 190-196. [pub] -

Language English Country United States

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc20028690

BACKGROUND: Europe is currently the most active region in the field of pancreatic islet transplantation, and many of the leading groups are actually achieving similar good outcomes. Further collaborative advances in the field require the standardization of islet cell product isolation processes, and this work aimed to identify differences in the human pancreatic islet isolation processes within European countries. METHODS: A web-based questionnaire about critical steps, including donor selection, pancreas processing, pancreas perfusion and digestion, islet counting and culture, islet quality evaluation, microbiological evaluation, and release criteria of the product, was completed by isolation facilities participating at the Ninth International European Pancreas and Islet Transplant Association (EPITA) Workshop on Islet-Beta Cell Replacement in Milan. RESULTS: Eleven islet isolation facilities completed the questionnaire. The facilities reported 445 and 53 islet isolations per year over the last 3 years from deceased organ donors and pancreatectomized patients, respectively. This activity resulted in 120 and 40 infusions per year in allograft and autograft recipients, respectively. Differences among facilities emerged in donor selection (age, cold ischemia time, intensive care unit length, amylase concentration), pancreas procurement, isolation procedures (brand and concentration of collagenase, additive, maximum acceptable digestion time), quality evaluation, and release criteria for transplantation (glucose-stimulated insulin secretion tests, islet numbers, and purity). Moreover, even when a high concordance about the relevance of one parameter was evident, thresholds for the acceptance were different among facilities. CONCLUSIONS: The result highlighted the presence of a heterogeneity in the islet cell product process and product release criteria.

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$a BACKGROUND: Europe is currently the most active region in the field of pancreatic islet transplantation, and many of the leading groups are actually achieving similar good outcomes. Further collaborative advances in the field require the standardization of islet cell product isolation processes, and this work aimed to identify differences in the human pancreatic islet isolation processes within European countries. METHODS: A web-based questionnaire about critical steps, including donor selection, pancreas processing, pancreas perfusion and digestion, islet counting and culture, islet quality evaluation, microbiological evaluation, and release criteria of the product, was completed by isolation facilities participating at the Ninth International European Pancreas and Islet Transplant Association (EPITA) Workshop on Islet-Beta Cell Replacement in Milan. RESULTS: Eleven islet isolation facilities completed the questionnaire. The facilities reported 445 and 53 islet isolations per year over the last 3 years from deceased organ donors and pancreatectomized patients, respectively. This activity resulted in 120 and 40 infusions per year in allograft and autograft recipients, respectively. Differences among facilities emerged in donor selection (age, cold ischemia time, intensive care unit length, amylase concentration), pancreas procurement, isolation procedures (brand and concentration of collagenase, additive, maximum acceptable digestion time), quality evaluation, and release criteria for transplantation (glucose-stimulated insulin secretion tests, islet numbers, and purity). Moreover, even when a high concordance about the relevance of one parameter was evident, thresholds for the acceptance were different among facilities. CONCLUSIONS: The result highlighted the presence of a heterogeneity in the islet cell product process and product release criteria.
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$a Kerr-Conte, Julie A $u European Genomic Institute for Diabetes, University Hospital Lille, Lille, France.
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$a Scholz, Hanne $u Department of Transplant Medicine, Oslo University Hospital, Oslo, Norway.
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$a Engelse, Marten $u Department of Nephrology, Leiden University Medical Center, Leiden, Netherlands.
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$a Karlsson, Marie $u Uppsala University Hospital, Uppsala, Sweden.
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$a Saudek, Frantisek $u Diabetes Center, Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
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$a Bosco, Domenico $u Hôpitaux Universitaires de Genève, Genève, Switzerland.
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$a Antonioli, Barbara $u Diabetology Unit, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy.
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$a Bertuzzi, Federico $u Diabetology Unit, ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy.
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$a Johnson, Paul R V $u Islet Transplant Research Group, Nuffield Department of Surgical Sciences and Oxford Centre for Diabetes, University of Oxford, Oxford, United Kingdom.
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$a Ludwing, Barbara $u Department of Medicine III, University Hospital Carl Gustav Carus Dresden, Dresden, Germany.
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$a Ling, Zhidong $u Academic Hospital and Diabetes Research Center, Vrije Universiteit Brussel, Brussels, Belgium.
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$a De Paep, Diedert L $u Academic Hospital and Diabetes Research Center, Vrije Universiteit Brussel, Brussels, Belgium.
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$a Keymeulen, Bart $u Academic Hospital and Diabetes Research Center, Vrije Universiteit Brussel, Brussels, Belgium.
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$a Pattou, François $u European Genomic Institute for Diabetes, University Hospital Lille, Lille, France.
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$a Berney, Thierry $u Hôpitaux Universitaires de Genève, Genève, Switzerland.
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$a Korsgren, Olle $u Uppsala University Hospital, Uppsala, Sweden.
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$a de Koning, Eelco $u Department of Nephrology, Leiden University Medical Center, Leiden, Netherlands.
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$a Piemonti, Lorenzo $u Diabetes Research Institute, IRCCS San Raffaele Scientific Institute, Milan, Italy. Vita-Salute San Raffaele University, Milan, Italy.
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