AIMS: Hofbauer cells (HBCs) are placental macrophages playing various roles during normal and complicated pregnancies, and of the latter, chorioamnionitis is the most frequent. METHODS: In placenta with chorioamnionitis, we examined immunohistochemical expression profiles of IL-1β, IL-10, and their potential regulators, CYP2C8 and soluble epoxide hydrolase (sEH), in Hofbauer cells and compared the results with our previously published data for normal placenta. RESULTS: We found that the expression profiles of the studied proteins in Hofbauer cells in chorioamnionitis differs from normal placenta. In chorioamnionitis, HBCs showed a moderate expression of IL-1β together with a weak expression of IL-10 and CYP2C8. Contrary to normal placenta, HBCs in chorioamnionitis express sEH. We demonstrated a moderate positive correlation between the expression of CYP2C8 and sEH in chorioamnionitis (Spearman r = 0.5654), suggesting enhanced degradation of anti-inflammatory epoxyeicosatrienoic acids. Moreover, the relations of IL-1β and IL-10 to CYP2C8, previously described in normal placenta, disappeared. Furthermore, a weak expression of anti-inflammatory IL-10 in chorioamnionitis was accompanied by change in circularity of HBCs (Spearman r = 0.8193). CONCLUSION: Taken together, these findings suggest a possible alteration of the anti-inflammatory role of HBCs and its regulation in chorioamnionitis.

Department of Histology and Embryology Faculty of Medicine and Dentistry Palacky University Olomouc Czech Republic

000      

00000naa a2200000 a 4500

001      

bmc22022919

003      

CZ-PrNML

005      

20230118155257.0

007      

ta

008      

221007s2022 xr ad f 000 0|eng||

009      

AR

024    7_

$a 10.5507/bp.2021.026 $2 doi

035    __

$a (PubMed)33976432

040    __

$a ABA008 $b cze $d ABA008 $e AACR2

041    0_

$a eng

044    __

$a xr

100    1_

$a Tauber, Zdeněk $u Department of Histology and Embryology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Czech Republic $7 xx0228394

245    14

$a The anti-inflammatory role of placental Hofbauer cells is altered in patients with chorioamnionitis: Are CYP2C8 and soluble epoxide hydrolase involved in immunomodulation / $c Z. Tauber, K. Cizkova

520    9_

$a AIMS: Hofbauer cells (HBCs) are placental macrophages playing various roles during normal and complicated pregnancies, and of the latter, chorioamnionitis is the most frequent. METHODS: In placenta with chorioamnionitis, we examined immunohistochemical expression profiles of IL-1β, IL-10, and their potential regulators, CYP2C8 and soluble epoxide hydrolase (sEH), in Hofbauer cells and compared the results with our previously published data for normal placenta. RESULTS: We found that the expression profiles of the studied proteins in Hofbauer cells in chorioamnionitis differs from normal placenta. In chorioamnionitis, HBCs showed a moderate expression of IL-1β together with a weak expression of IL-10 and CYP2C8. Contrary to normal placenta, HBCs in chorioamnionitis express sEH. We demonstrated a moderate positive correlation between the expression of CYP2C8 and sEH in chorioamnionitis (Spearman r = 0.5654), suggesting enhanced degradation of anti-inflammatory epoxyeicosatrienoic acids. Moreover, the relations of IL-1β and IL-10 to CYP2C8, previously described in normal placenta, disappeared. Furthermore, a weak expression of anti-inflammatory IL-10 in chorioamnionitis was accompanied by change in circularity of HBCs (Spearman r = 0.8193). CONCLUSION: Taken together, these findings suggest a possible alteration of the anti-inflammatory role of HBCs and its regulation in chorioamnionitis.

650    _2

$a antiflogistika $7 D000893

650    12

$a chorioamnionitida $7 D002821

650    12

$a cytochrom P450 CYP2C8 $x metabolismus $7 D065727

650    12

$a epoxid hydrolasy $x metabolismus $7 D004851

650    _2

$a ženské pohlaví $7 D005260

650    _2

$a lidé $7 D006801

650    _2

$a imunomodulace $7 D056747

650    _2

$a interleukin-10 $7 D016753

650    _2

$a placenta $x metabolismus $7 D010920

650    _2

$a těhotenství $7 D011247

655    _2

$a časopisecké články $7 D016428

700    1_

$a Čížková, Kateřina $u Department of Histology and Embryology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Czech Republic $7 xx0249672

773    0_

$w MED00012606 $t Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia $x 1804-7521 $g Roč. 166, č. 3 (2022), s. 267-273

856    41

$u https://pubmed.ncbi.nlm.nih.gov/33976432 $y Pubmed

910    __

$a ABA008 $b A 1502 $c 958 $y p $z 0

990    __

$a 20221007 $b ABA008

991    __

$a 20230118155252 $b ABA008

999    __

$a ok $b bmc $g 1885625 $s 1174205

BAS    __

$a 3

BAS    __

$a PreBMC

BMC    __

$a 2022 $b 166 $c 3 $d 267-273 $e 20210511 $i 1804-7521 $m Biomedical papers of the Medical Faculty of the University Palacký, Olomouc Czech Republic $n Biomed. Pap. Fac. Med. Palacký Univ. Olomouc Czech Repub. (Print) $x MED00012606

LZP    __

$b NLK138 $a Pubmed-20221007